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Monoclonal Mouse Anti-Human
Epithelial Membrane Antigen (EMA)
Clone E29
Labels normal and neoplastic epithelium.
The antibody is very useful together with the monoclonal antibodies to leucocyte common antigen (Code M0701) and cytokeratin (Code M0821), which label cells of lymphoid and epithelial origin, respectively. In the classification of hematolymphoid neoplasms, the antibody labels Reed-Sternberg cells in nodular lymphocyte predominant Hodgkin's lymphoma and neoplastic cells in a subset of anaplastic large cell lymphomas.
Isotype IgG2a, kappa
- Frozen
- Formalin
- Enzyme/HIER
Breast ductal carcinoma (FFPE) stained with FLEX Anti-EMA, Code IR629/IS629.
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Breast ductal carcinoma. The majority of the neoplastic cells show a moderate to strong membranous and cytoplasmic staining reaction.
Clinical application for IR629/IS629 For identification of tumors of epithelial origin in a wide variety of tissues.
Mesothelioma. The malignant mesothelioma cells show a strong staining reaction in membrane and cytoplasm.
Clinical application for IR629/IS629 For identification of tumors of epithelial origin in a wide variety of tissues.
Breast. Epithelial cells show a strong staining reaction on the apical part of the cell membrane and, occasionally, on the entire circumference of the cell membrane. A granular cytoplasmic staining reaction can also be observed.
Recommended control for IR629/IS629. Breast/Tonsil.
Tonsil. The squamous epithelial cells show a moderate to strong distinct cytoplasmic staining reaction, and the plasma cells show a weak to moderate predominantly membranous staining reaction.
Recommended control for IR629/IS629. Breast/Tonsil.
Colon adenocarcinoma stained with Anti-EMA.
Clinical application for IR629/IS629 For identification of tumors of epithelial origin in a wide variety of tissues.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human Epithelial Membrane Antigen, Clone E29, is intended for use in immunohistochemistry. The antibody labels epithelial cells in a wide variety of tissues and is a useful tool for the identification of neoplastic epithelia (1). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human Epithelial Membrane Antigen, Clone E29, is intended for use in immunohistochemistry. The antibody labels epithelial cells in a wide variety of tissues and is a useful tool for the identification of neoplastic epithelia (1). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Human milk fat globule membrane preparation (1, 4).
Specificity
In Western blotting of the immunogen the antibody labels bands of 265-400 kDa (1)
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: E29 (1). Isotype: IgG2a, kappa.
Mouse IgG concentration: see label on vial.
Clone: E29 (1). Isotype: IgG2a, kappa.
Mouse IgG concentration: see label on vial.
References
- Cordell J, Richardson TC, Pulford KAF, Ghosh AK, Gatter KC, Heyderman E, et al. Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry. Br J Cancer 1985;52:347-54.
- Heyderman E, Strudley I, Powell G, Richardson TC, Cordell JL, Mason DY. A new monoclonal antibody to epithelial membrane antigen (EMA) - E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2. Br J Cancer 1985;52:355-61.
| Product | Size |  | M0613 Culture supernatant
| 0.2 mL Product No. M061329 |
|---|---|---|
| 1 mL Product No. M061301 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Epithelial Membrane Antigen, Clone E29, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of epithelial cells in a wide variety of tissues and is a useful tool for the identification of neoplastic epithelia (1). EMA is present on the membrane of secretory epithelia. The absence of EMA staining with monoclonal mouse anti-human epithelial membrane antigen, clone E29 is considered to be strongly suggestive of non-epithelial neoplasia (2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Epithelial Membrane Antigen, Clone E29, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of epithelial cells in a wide variety of tissues and is a useful tool for the identification of neoplastic epithelia (1). EMA is present on the membrane of secretory epithelia. The absence of EMA staining with monoclonal mouse anti-human epithelial membrane antigen, clone E29 is considered to be strongly suggestive of non-epithelial neoplasia (2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Human milk fat globule membrane preparation (1, 4).
Specificity
In Western blotting of the immunogen the antibody labels bands of 265-400 kDa (1).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
Clone: E29. Isotype: Ig2a, kappa.
Clone: E29. Isotype: Ig2a, kappa.
References
- Cordell J, Richardson TC, Pulford KAF, Ghosh AK, Gatter KC, Heyderman E, et al. Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry. Br J Cancer 1985;52:347-54.
- Gatter KC, Heryet A, Alcock C, Mason DY. Clinical importance of analyzing malignant tumors of uncertain origin with immunohistological techniques. Lancet 1985; June:1302
- Sloane JP, Ormerod MG. Distribution of epithelial membrane antigen in normal and neoplastic tissues and its value in diagnostic tumor pathology. Cancer 1981:47:1786-95.
| Product | Size | | IR629 RTU, FLEX
| 60 tests, 12 mL Product No. IR62961 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Tonsil | 120 tests, 24 mL Product No. IR75066 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Epithelial Membrane Antigen, Clone E29, Ready-to-Use (Dako Autostainer/Autostainer Plus) is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of epithelial cells in a wide variety of tissues and is a useful tool for the identification of neoplastic epithelia (1). EMA is present on the membrane of secretory epithelia. The absence of EMA staining with monoclonal mouse anti-human epithelial membrane antigen, clone E29 is considered to be strongly suggestive of non-epithelial neoplasia (2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Epithelial Membrane Antigen, Clone E29, Ready-to-Use (Dako Autostainer/Autostainer Plus) is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of epithelial cells in a wide variety of tissues and is a useful tool for the identification of neoplastic epithelia (1). EMA is present on the membrane of secretory epithelia. The absence of EMA staining with monoclonal mouse anti-human epithelial membrane antigen, clone E29 is considered to be strongly suggestive of non-epithelial neoplasia (2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Human milk fat globule membrane preparation (1, 4).
Specificity
In Western blotting of the immunogen the antibody labels bands of 265-400 kDa (1).
Reagent Provided
Ready-to-Use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
Clone: E29 Isotype: Ig2a, kappa.
Clone: E29 Isotype: Ig2a, kappa.
References
- Cordell J, Richardson TC, Pulford KAF, Ghosh AK, Gatter KC, Heyderman E, et al. Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry. Br J Cancer 1985;52:347-54.
- Gatter KC, Heryet A, Alcock C, Mason DY. Clinical importance of analyzing malignant tumors of uncertain origin with immunohistological techniques. Lancet 1985; June:1302
- Sloane JP, Ormerod MG. Distribution of epithelial membrane antigen in normal and neoplastic tissues and its value in diagnostic tumor pathology. Cancer 1981:47:1786-95.
| Product | Size | | IS629 RTU, FLEX
| 30 tests, 6 mL Product No. IS62930 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Tonsil | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
Synonym(s) for Antigen
EMA, MUC-1, MUC1