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Monoclonal Mouse Anti-
Vimentin
Clone V9
Vimentin is a 57 kDa intermediate filament protein which forms part of the cytoskeleton of vertebrate cells and is characteristically found in cells of mesenchymal origin.
The coexpression of intermediate filaments, particularly vimentin and cytokeratin, has been demonstrated in a variety of normal cells/tissues and in neoplastic lesions, necessitating the use of antibodies against other types of intermediate filaments. The antibody is useful for the identification of cells of mesenchymal origin in normal and neoplastic tissues.
B-cell chronic lymphocytic leukemia (FFPE) stained with FLEX Anti-Vimentin, Code IR630/ IS630.
B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma. The majority of the neoplastic cells show a distinct cytoplasmic staining reaction
Clinical application for IR630/IS630. For identification of cells of mesenchymal origin in normal and neoplastic tissues such as B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
FLEX Monoclonal Mouse Anti-Vimentin, Clone V9, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of cells of mesenchymal origin in normal and neoplastic tissues, and is of value in tumor diagnosis. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
In immunocytochemistry the antibody labels the vimentin-positive human cell lines IMR90, RD, glioma and HeLa (3).
Clone: V9 (3). Isotype: IgG1, kappa.
- Osborn M, Debus E, Weber K. Monoclonal antibodies specific for vimentin. Eur J Cell Biol 1984;34:137-43.
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| Product | Size |  | IS630 Clone V9
| 30 tests, 6 mL Product No. IS63030-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Tonsil and liver | 60 tests, 12 mL Product No. IS75061-2 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021-2 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |