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Monoclonal Mouse Anti-Human
Placental Alkaline Phosphatase
Clone 8A9
The immunohistochemical detection of placental alkaline phosphatase has been shown to be of great value in the evaluation of many different types of germ cell neoplasia and carcinomas of the lung, stomach, pancreas, breast and ovary.
Seminoma (FFPE) stained with FLEX Anti-Placental Alkaline Phosphatase, Code IR779/IS779.
Seminoma. The majority of the neoplastic cells show a moderate to strong predominantly membranous, but also cytoplasmic staining reaction.
Clinical application for IR779/IS779 For identification of malignant germ cell tumors such as dysgerminoma and embryonal carcinoma.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Placental Alkaline Phosphatase, Clone 8A9, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/ Autostainer Plus instruments. This antibody labels cells expressing placental alkaline phosphatase (PLAP) and PLAP-like alkaline phosphatase, also named germ cell AP (GCAP) (1). This antibody is useful for the identification of seminomas (2) and desmoplastic small round cell tumors (3). Antibodies to PLAP/GCAP are particularly useful for the identification of germ cell tumors (4-6). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Placental Alkaline Phosphatase, Clone 8A9, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/ Autostainer Plus instruments. This antibody labels cells expressing placental alkaline phosphatase (PLAP) and PLAP-like alkaline phosphatase, also named germ cell AP (GCAP) (1). This antibody is useful for the identification of seminomas (2) and desmoplastic small round cell tumors (3). Antibodies to PLAP/GCAP are particularly useful for the identification of germ cell tumors (4-6). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Purified human placental alkaline phosphatase.
Specificity
As demonstrated by immunohistochemistry, the antibody labels seminomas and placenta, recognising possibly both PLAP and GCAP.
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: 8A9. Isotype: IgG1, kappa.
Clone: 8A9. Isotype: IgG1, kappa.
References
- Millán JL, Fishman WH. Biology of human alkaline phosphatases with special reference to cancer. Crit Rev Clin Lab Sci 1995;32:1-39.
- Eyzaguirre E, Gatalica Z. Loss of fhit expression in testicular germ cell tumors and intratubular germ cell neoplasia. Mod Pathol 2002;15:1068-72.
- Zhang PJ, Golfblum JR, Pawel BR, Fischer C, Pasha TL, Barr FG. Immunophenotype of desmoplastic small round cell tumors as detected in cases with EWS-WT1 gene fusion product. Mod Pathol 2003;16:229-35.
- Leong AS-Y, Cooper K, Leong FJW-M. Manual of diagnostic antibodies for immunohistology. London: Oxford University Press; 1999. p. 277-8.
- Dieckmann K-P, Skakkebaek NE. Carcinoma in situ of the testis: review of biological and clinical features. Int J Cancer 1999;83:815-22.
- Hamilton-Dutoit SJ, Lou H, Pallesen G. The expression of placental alkaline phosphatase (PLAP) and PLAP-like enzymes in normal and neoplastic human tissues. APMIS 1990;98:797-811.
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| Product | Size |  | IS779 Clone 8A9
| 30 tests, 6 mL Product No. IS77930-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Placenta | 60 tests, 12 mL Product No. IS75061-2 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021-2 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |