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Monoclonal Mouse Anti-Human
CD45, Leucocyte Common Antigen
Clone 2B11 + PD7/26
Reacts with CD45 in both normal and neoplastic cells.
Together with other antibodies, it is useful for differentiation of hematopoietic neoplasms, particularly of the lymphoid type, from poorly differentiated epithelial and mesenchymal neoplasms. Antibodies to CD45 may also aid in the diagnosis of lymphocyte predominant Hodgkin's disease.
Isotype IgG1, kappa + IgG1, kappa
- Frozen
- Formalin
- HIER
References
- Kurtin PJ, Pinkus GS. Leukocyte common antigen - a diagnostic discriminant between hematopoietic and nonhematopoietic neoplasms in paraffin sections using monoclonal antibodies: correlation with immunologic studies and ultrastructural localization. Hum Pathol 1985;16:353.
- Michie SA, Spagnolo DV, Dunn KA, Warnke RA, Rouse RV. A panel approach to the evaluation of the sensitivity and specificity of antibodies for the diagnosis of routinely processed histologically undifferentiated human neoplasms. Am J Clin Pathol 1987;88:457-62.
Acute myeloid leukemia (FFPE) stained with FLEX Anti-CD45, Code IR751/IS751.
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Acute myeloid leukemia. The neoplastic cells show a strong membranous staining reaction.
Clinical application for IR751/IS751. For identifying tumors of lymphoid origin.
B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma. The majority of the neoplastic cells show a strong membranous staining reaction.
Clinical application for IR751/IS751. For identifying tumors of lymphoid origin.
Tonsil. Lymphocytes show a strong membranous staining reaction, whereas histiocytes and macrophages show a weak to moderate membranous staining reaction. Surface epithelial cells should be negative.
Recommended control for IR751/IS751. Tonsil/Brain/Bone Marrow.
Cerebellum. The microglial cells show a weak to moderate and predominantly membranous staining reaction.
Recommended control for IR751/IS751. Tonsil/Brain/Bone Marrow.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human CD45, Leucocyte Common Antigen, Clones 2B11 + PD7/26, is intended for use in immunohistochemistry. The antibody labels CD45 in both normal and neoplastic cells, and is a useful tool for identifying tumor cells of lymphoid origin (1-3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human CD45, Leucocyte Common Antigen, Clones 2B11 + PD7/26, is intended for use in immunohistochemistry. The antibody labels CD45 in both normal and neoplastic cells, and is a useful tool for identifying tumor cells of lymphoid origin (1-3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
2B11: Isolated neoplastic cells from a case of T-cell lymphoma/leukemia (1).
PD7/26: Human peripheral blood lymphocytes maintained in T-cell growth factor (1).
PD7/26: Human peripheral blood lymphocytes maintained in T-cell growth factor (1).
Specificity
Anti-CD45 is a mixture of two monoclonal antibodies, clones 2B11 and PD7/26, directed against different epitopes. Clone 2B11 was clustered as anti-CD45 at the Third International Workshop and Conference on Human Leucocyte Differentiation Antigens, held in Oxford in 1986 and reacts with all the known isotypes of the CD45 family (5). Clone PD7/26 was clustered as anti-CD45RB at the Fifth International Workshop and Conference on Human Leucocyte Differentiation Antigens, held in Boston in 1993 (6).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clones: 2B11 (1) and PD7/26 (1). Isotype: IgG1, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
Clones: 2B11 (1) and PD7/26 (1). Isotype: IgG1, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
References
- Warnke RA, Gatter KC, Falini B, Hildreth P, Woolston R-E, Pulford K, et al. Diagnosis of human lymphoma with monoclonal antileukocyte antibodies. N Engl J Med 1983;309:1275-81.
- Kurtin PJ, Pinkus GS. Leukocyte common antigen - a diagnostic discriminant between hematopoietic and nonhematopoietic neoplasms in paraffin sections using monoclonal antibodies: Correlation with immunologic studies and ultrastructural localization. Hum Pathol 1985;16:353.
- Michie SA, Spagnolo DV, Dunn KA, Warnke RA, Rouse RV. A panel approach to the evaluation of the sensitivity and specificity of antibodies for the diagnosis of routinely processed histologically undifferentiated human neoplasms. Am J Clin Pathol 1987;88:457-62.
- Cobbold S, Hale G, Waldmann H. Non-lineage, LFA-1, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ, Beverley PCL, Cobbold S, Crumpton MJ, Gilks W, Gotch FM, et al., editors. Leukocyte typing III. White cell differentiation antigens. Proceedings of the 3rd International Workshop and Conference; 1986 Sep 21-26; Oxford, England. Oxford, New York, Tokyo: Oxford University Press; 1987. p. 788-803.
- Morimoto C. T18. CD45 cluster report. In: Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, et al., editors. Leukocyte typing V. White cell differentiation antigens. Proceedings of the 5th International Workshop and Conference; 1993 Nov 3-7; Boston, USA. Oxford, New York, Tokyo: Oxford University Press; 1995. p. 386-9.
| Product | Size |  | M0701 Culture supernatant
| 0.2 mL Product No. M070129 |
|---|---|---|
| 1 mL Product No. M070101 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human CD45, Leucocyte Common Antigen, Clones 2B11 + PD7/26, Ready-to-Use, (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels CD45 in both normal and neoplastic cells, and is useful for identifying tumor cells of lymphoid origin (1, 2, 3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human CD45, Leucocyte Common Antigen, Clones 2B11 + PD7/26, Ready-to-Use, (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels CD45 in both normal and neoplastic cells, and is useful for identifying tumor cells of lymphoid origin (1, 2, 3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
2B11: Isolated neoplastic cells from a case of T-cell lymphoma/leukemia (1).
PD7/26: Human peripheral blood lymphocytes maintained in T-cell growth factor (1).
PD7/26: Human peripheral blood lymphocytes maintained in T-cell growth factor (1).
Specificity
Anti-CD45 is a mixture of two monoclonal antibodies, clones 2B11 and PD7/26, directed against different epitopes. Clone 2B11 was clustered as anti-CD45 at the Third International Workshop and Conference on Human Leucocyte Differentiation Antigens and reacts with all the known isotypes of the CD45 family (6). Clone PD7/26 was clustered as anti-CD45RB at the Fifth International Workshop and Conference on Human Leucocyte Differentiation Antigens (7).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clones: 2B11 (1) and PD7/26 (1). Isotype: IgG1, kappa
Clones: 2B11 (1) and PD7/26 (1). Isotype: IgG1, kappa
References
- Warnke RA, Gatter KC, Falini B, Hildreth P, Woolston R-E, Pulford K, et al. Diagnosis of human lymphoma with monoclonal antileukocyte antibodies. N Engl J Med 1983;309:1275-81.
- Kurtin PJ, Pinkus GS. Leukocyte common antigen - a diagnostic discriminant between hematopoietic and nonhematopoietic neoplasms in paraffin sections using monoclonal antibodies: Correlation with immunologic studies and ultrastructural localization. Hum Pathol 1985;16:353-65
- Michie SA, Spagnolo DV, Dunn KA, Warnke RA, Rouse RV. A panel approach to the evaluation of the sensitivity and specificity of antibodies for the diagnosis of routinely processed histologically undifferentiated human neoplasms. Am J Clin Pathol 1987; 88:457-62.
- Cobbold S, Hale G, Waldmann H. Non-lineage, LFA-1, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ, Beverley PCL, Cobbold S, Crumpton MJ, Gilks W, Gotch FM, et al., editors. Leukocyte typing III. White cell differentiation antigens. Proceedings of the 3rd International Workshop and Conference; 1986 Sep 21-26; Oxford, England. Oxford, New York, Tokyo: Oxford University Press; 1987. p. 788-803.
- Morimoto C. T18. CD45 cluster report. In: Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, et al., editors. Leukocyte typing V. White cell differentiation antigens. Proceedings of the 5th International Workshop and Conference; 1993 Nov 3-7; Boston, USA. Oxford, New York, Tokyo: Oxford University Press; 1995. p. 386-9.
| Product | Size | | IR751 RTU, FLEX
| 60 tests, 12 mL Product No. IR75161 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Tonsil and brain | 120 tests, 24 mL Product No. IR75066 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human CD45, Leucocyte Common Antigen, Clones 2B11 + PD7/26, Ready-to-Use, (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with (Dako Autostainer/Autostainer Plus instruments. This antibody labels CD45 in both normal and neoplastic cells, and is useful for identifying tumor cells of lymphoid origin (1-3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human CD45, Leucocyte Common Antigen, Clones 2B11 + PD7/26, Ready-to-Use, (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with (Dako Autostainer/Autostainer Plus instruments. This antibody labels CD45 in both normal and neoplastic cells, and is useful for identifying tumor cells of lymphoid origin (1-3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
2B11: Isolated neoplastic cells from a case of T-cell lymphoma/leukemia (1).
PD7/26: Human peripheral blood lymphocytes maintained in T-cell growth factor (1).
PD7/26: Human peripheral blood lymphocytes maintained in T-cell growth factor (1).
Specificity
Anti-CD45 is a mixture of two monoclonal antibodies, clones 2B11 and PD7/26, directed against different epitopes. Clone 2B11 was clustered as anti-CD45 at the Third International Workshop and Conference on Human Leucocyte Differentiation Antigens and reacts with all the known isotypes of the CD45 family (6). Clone PD7/26 was clustered as anti-CD45RB at the Fifth International Workshop and Conference on Human Leucocyte Differentiation Antigens (7).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clones: 2B11 (1) and PD7/26 (1). Isotype: IgG1, kappa.
Clones: 2B11 (1) and PD7/26 (1). Isotype: IgG1, kappa.
References
- Warnke RA, Gatter KC, Falini B, Hildreth P, Woolston R-E, Pulford K, et al. Diagnosis of human lymphoma with monoclonal antileukocyte antibodies. N Engl J Med 1983;309:1275-81.
- Kurtin PJ, Pinkus GS. Leukocyte common antigen - a diagnostic discriminant between hematopoietic and nonhematopoietic neoplasms in paraffin sections using monoclonal antibodies: Correlation with immunologic studies and ultrastructural localization. Hum Pathol 1985;16:353-65
- Michie SA, Spagnolo DV, Dunn KA, Warnke RA, Rouse RV. A panel approach to the evaluation of the sensitivity and specificity of antibodies for the diagnosis of routinely processed histologically undifferentiated human neoplasms. Am J Clin Pathol 1987; 88:457-62.
- Cobbold S, Hale G, Waldmann H. Non-lineage, LFA-1, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ, Beverley PCL, Cobbold S, Crumpton MJ, Gilks W, Gotch FM, et al., editors. Leukocyte typing III. White cell differentiation antigens. Proceedings of the 3rd International Workshop and Conference; 1986 Sep 21-26; Oxford, England. Oxford, New York, Tokyo: Oxford University Press; 1987. p. 788-803.
- Morimoto C. T18. CD45 cluster report. In: Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, et al., editors. Leukocyte typing V. White cell differentiation antigens. Proceedings of the 5th International Workshop and Conference; 1993 Nov 3-7; Boston, USA. Oxford, New York, Tokyo: Oxford University Press; 1995. p. 386-9.
| Product | Size | | IS751 RTU, FLEX
| 30 tests, 6 mL Product No. IS75130 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Tonsil and brain | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
Synonym(s) for Antigen
ly-5, T200, LCA