Belgium
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Monoclonal Mouse Anti-
Enterovirus
Clone 5-D8/1
Reacts with an epitope on the VP1 peptide which is highly conserved within the enterovirus group. The antibody, originally generated using coxsackie virus B5 as immunogen, reacts with most of the enterovirus strains of the coxsackie, echo and poliovirus groups. No reaction is seen with human rotavirus, yellow fever virus, measles virus, rhinovirus A1 and adenovirus 18. M7064 is especially suited for testing of cell cultures inoculated with samples from patients suspected of having an enterovirus infection. When using M7064 in the indirect immunofluorescence technique, we recommend polyclonal rabbit anti-mouse immunoglobulins/FITC as secondary antibody.
Isotype IgG2a, kappa
- Frozen
- Formalin
References
- Yousef GE, Brown IN, Mowbray JF. Derivation and biochemical characterization of an enterovirus group-specific monoclonal antibody. Intervirology 1987;28:163-70.
- Bourlet T, Gharbi J, Omar S, Aouni M, Pozzetto B. Comparison of a rapid culture method combining an immunoperoxidase test and a group specific anti-VP1 monoclonal antibody with conventional virus isolation techniques for routine detection of enteroviruses in stools. J Med Virol 1998;54:204-9.
- Excellent specificity
- High lot to lot consistency
- Certified manufacturing facilities guarantee full quality control
Intended Use
Analyte specific reagent. Analytical and performance characteristics are not established.
The antibody reacts with an epitope on the VP1 peptide, which is highly conserved within the enterovirus group (1). Western blots have shown that the antibody reacts with a single peptide having a molecular mass of between 34 and 37 kDa.
The antibody reacts with an epitope on the VP1 peptide, which is highly conserved within the enterovirus group (1). Western blots have shown that the antibody reacts with a single peptide having a molecular mass of between 34 and 37 kDa.
Immunogen
Heat-inactivated purified coxsackie B5 virus (1).
Reagent Provided
Monoclonal mouse antibody supplied in liquid form as cell culture supernatant (RPMI 1640 medium containing foetal calf serum), dialysed against 0.05 mol/L Tris/HCl pH 7.2, 15 mmol/L NaN3.
Clone: 5-D8/1(1). Isotype: IgG2a, kappa.
Mouse Ig concentration: see label on vial.
Clone: 5-D8/1(1). Isotype: IgG2a, kappa.
Mouse Ig concentration: see label on vial.
References
- Yousef GE, Brown IN, Mowbray JF. Derivation and biochemical characterization of an enterovirus group-specific monoclonal antibody. Intervirology 1987;28:163-70.
| Product | Size |  | M7064 Culture supernatant
| 1 mL Product No. M706401 |
|---|