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Polyclonal Rabbit Anti-
Helicobacter Pylori
H. pylori (formerly called Campylobacter pylori) is a spiral-shaped microaerophilic, Gram-negative rod with unipolar sheated flagella.
It inhabits the mucus lining of the gastric epithelium. H. pylori is one of the most common pathogenic infections, which leads to serious gastroduodenal diseases in a subset of individuals. There is evidence supporting an association between H. pylori and chronic atrophic gastritis as well as gastric cancer. This antibody is useful for the identification of infections with H. pylori in gastritis and gastric cancer.
- Formalin
- Enzyme/HIER
Gastric mucosa (FFPE) stained with FLEX Anti-Helicobacter Pylori, Code IR523/IS523.
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Gastric mucosa infected with Helicobacter pylori. Helicobacter pylori in the gastric mucosa lining show a moderate to strong staining reaction.
Clinical application for IR523/IS523 For identification of Helicobacter pylori infection in gastritis and primary gastric lymphomas.
Gastric mucosa infected with Helicobacter pylori. Helicobacter pylori in the gastric mucosa lining show a moderate to strong staining reaction.
Clinical application for IR523/IS523 For identification of Helicobacter pylori infection in gastritis and primary gastric lymphomas.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Polyclonal Rabbit Anti-Helicobacter Pylori, is intended for use in immunohistochemistry. The antibody labels the bacterium Helicobacter pylori (H. pylori), and may be a useful tool for identification of infections with H. pylori in gastritis and gastric cancer (1-4). Differential identification is aided by the results from a panel of antibodies. Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Polyclonal Rabbit Anti-Helicobacter Pylori, is intended for use in immunohistochemistry. The antibody labels the bacterium Helicobacter pylori (H. pylori), and may be a useful tool for identification of infections with H. pylori in gastritis and gastric cancer (1-4). Differential identification is aided by the results from a panel of antibodies. Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Immunogen
The antigen for immunization was prepared from heat-treated cells of the H. pylori strain, CH-20426 (1, 2).
Specificity
The antibody labels heat-stable antigens of the H. pylori organism. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins.
In crossed immunoelectrophoresis, using 12.5 µL antibody per cm 2 gel area against 2 µL of human plasma no precipitate appears. Staining: Coomassie Brilliant Blue.
In crossed immunoelectrophoresis, using 12.5 µL antibody per cm 2 gel area against 2 µL of human plasma no precipitate appears. Staining: Coomassie Brilliant Blue.
Reagent Provided
Purified immunoglobulin fraction of rabbit antiserum provided in liquid form in 0.1 mol/L NaCl, 15 mmol/L NaN3.
Protein concentration: See label on vial.
Protein concentration: See label on vial.
References
- Andersen LP, Holck S, Povlsen CO. Campylobacter pylori detected by indirect immunohistochemical technique. Apmis 1988;96:559-64.
- Andersen LP, Holck S. Possible evidence of invasiveness of Helicobacter (Campylobacter) pylori. Eur J Clin Microbiol Infect Dis 1990;9:135-8.
- Jonkers D, Gisbertz I, de Bruine A, Bot F, Arends JW, Stobberingh E, et al. Helicobacter pylori and non-Helicobacter pylori bacterial flora in gastric mucosal and tumour specimens of patients with primary gastric lymphoma. Eur J Clin Invest 1997;27:885-92.
- Jonkers D, Stobberingh E, de Bruine A, Arends JW, Stockbrügger R. Evaluation of immunohistochemistry for the detection of Helicobacter pylori in gastric mucosal biopsies. J Infect 1997;35:149-54.
| Product | Size |  | B0471 Ig fraction
| 0.2 mL Product No. B047129 |
|---|---|---|
| 1 mL Product No. B047101 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Helicobacter Pylori, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of infections with H. pylori in gastritis and gastric cancer (1-4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Polyclonal Rabbit Anti-Helicobacter Pylori, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of infections with H. pylori in gastritis and gastric cancer (1-4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
The antigen for immunization was prepared from heat-treated cells of the H. pylori strain, CH-20426 (1, 2).
Specificity
The antibody labels heat-stable, somatic antigens of the whole H. pylori organism. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins.
In crossed immunoeletrophoresis, using 12.5 µL concentrated antibody per cm2 gel area against 2 µL of human plasma no precipitate appears. Staining: Coomassie Brilliant Blue.
In crossed immunoeletrophoresis, using 12.5 µL concentrated antibody per cm2 gel area against 2 µL of human plasma no precipitate appears. Staining: Coomassie Brilliant Blue.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.1 mol/L NaCl, 0.015 mol/L NaN3.
References
- Anderson LP, Holck S, Povlsen CO. Campylobacter pylori detected by indirect immunohistochemical technique. Ampis 1988;96:559-64.
- Anderson LP, Holck S. Possible evidence of invasiveness of Helicobacter (Campylobacter) pylori. Eur J Clin Microbiol Infect Dis 1990;9:135-8.
- Jonkers D, Gisbertz I, de Bruine A, Bot F, Arends JW, Stobberingh E, et al. Helicobacter pylori and non- Helicobacter pylori bacterial flora in gastric mucosal and tumor specimens of patients with primary gastric lymphoma. Eur J Clin Invest 1997;27:885-92.
- Jonkers D, Stobberingh E, de Bruine A, Arends JW, Stockbrügger R. Evaluation of Immunohistochemistry for the detection of Helicobacter pylori in gastric mucosal biopsies. J Infect 1997;35:149-54.
| Product | Size | | IR523 RTU, FLEX
| 60 tests, 12 mL Product No. IR52361 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Gastric mucosa with H. Pylori infection | 120 tests, 24 mL Product No. IR75066 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Helicobacter Pylori, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of infections with H. pylori in gastritis and gastric cancer (1-4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Polyclonal Rabbit Anti-Helicobacter Pylori, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of infections with H. pylori in gastritis and gastric cancer (1-4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
The antigen for immunization was prepared from heat-treated cells of the H. pylori strain, CH-20426 (1, 2).
Specificity
The antibody labels heat-stable, somatic antigens of the whole H. pylori organism. Traces of contaminating antibodies have been found by solid-phase absorption with human plasma proteins.
In crossed immunoeletrophoresis, using 12.5 µL concentrated antibody per cm2 gel area against 2 µL of human plasma no precipitate appears. Staining: Coomassie Brilliant Blue.
In crossed immunoeletrophoresis, using 12.5 µL concentrated antibody per cm2 gel area against 2 µL of human plasma no precipitate appears. Staining: Coomassie Brilliant Blue.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.1 mol/L NaCl, 0.015 mol/L NaN3.
References
- Anderson LP, Holck S, Povlsen CO. Campylobacter pylori detected by indirect immunohistochemical technique. Ampis 1988;96:559-64.
- Anderson LP, Holck S. Possible evidence of invasiveness of Helicobacter (Campylobacter) pylori. Eur J Clin Microbiol Infect Dis 1990;9:135-8.
- Jonkers D, Gisbertz I, de Bruine A, Bot F, Arends JW, Stobberingh E, et al. Helicobacter pylori and non- Helicobacter pylori bacterial flora in gastric mucosal and tumor specimens of patients with primary gastric lymphoma. Eur J Clin Invest 1997;27:885-92.
- Jonkers D, Stobberingh E, de Bruine A, Arends JW, Stockbrügger R. Evaluation of Immunohistochemistry for the detection of Helicobacter pylori in gastric mucosal biopsies. J Infect 1997;35:149-54.
| Product | Size | | IS523 RTU, FLEX
| 30 tests, 6 mL Product No. IS52330 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Gastric mucosa with H. Pylori infection | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |