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Polyclonal Rabbit Anti-Human
Von Willebrand Factor
The antibody is a useful tool for studying angiogenesis in neoplasms such as breast cancer (1) and for the identification of epithelioid hemangioendothelioma (2).
The former designation for von Willebrand factor was factor VIII-related antigen.
- Formalin
- Enzyme/HIER
References
- Weidner N, Semple JP, Welch WR, Folkman J. Tumor angiogenesis and metastasis - correlation in invasive breast carcinoma. N Engl J Med 1991;324:1-8.
- Makhlouf HR, Ihsak KD, Goodman ZD. Epithelioid hemangioendothelioma of the liver: a clinicopathologic study of 137 cases. Cancer 1999;85:562-82.
Hemangiosarcoma (FFPE) stained with FLEX Anti-Von Willebrand Factor, Code IR527/IS527.
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Hemangiosarcoma. The majority of the neoplastic cells show a moderate to strong predominantly cytoplasmic staining reaction.
Clinical application for IR527/IS527. For identification of acute myeloid leukemia FAB type M7 and angiosarcoma.
Acute myeloid leukemia (FAB type M7). The neoplastic cells show a strong predominantly cytoplasmic staining reaction.
Clinical application for IR527/IS527. For identification of acute myeloid leukemia FAB type M7 and angiosarcoma.
Colon. The endothelial cells of both blood and lymphatic vessels in lamina propria show a moderate to strong staining reaction. The epithelial cells should be negative.
Recommended control for IR527/IS527. Colon/Liver.
Liver. The endothelial cells lining the sinusoids show a weak to moderate staining reaction. The liver cells should be negative or only focally positive.
Recommended control for IR527/IS527. Colon/Liver.
Breast carcinoma stained with Anti-Von Willebrand Factor.
Clinical application for IR527/IS527. For identification of acute myeloid leukemia FAB type M7 and angiosarcoma.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Polyclonal Rabbit Anti-Human Von Willebrand Factor, is intended for use in immunohistochemistry. The antibody is also well-suited for ELISA and gel immunoprecipitation techniques. The antibody is a useful tool for studying angiogenesis in neoplasms such as breast cancer (1) and for the identification of epithelioid hemangioendothelioma (2). Differential identification is aided by the results from a panel of antibodies. For the specific and quantitative determination of von Willebrand Factor circulating in plasma, an enzyme linked immunosorbent assay (ELISA) may be a useful tool in the determination of von Willebrand disease (3). Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Polyclonal Rabbit Anti-Human Von Willebrand Factor, is intended for use in immunohistochemistry. The antibody is also well-suited for ELISA and gel immunoprecipitation techniques. The antibody is a useful tool for studying angiogenesis in neoplasms such as breast cancer (1) and for the identification of epithelioid hemangioendothelioma (2). Differential identification is aided by the results from a panel of antibodies. For the specific and quantitative determination of von Willebrand Factor circulating in plasma, an enzyme linked immunosorbent assay (ELISA) may be a useful tool in the determination of von Willebrand disease (3). Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Immunogen
Von Willebrand Factor isolated from human plasma.
Specificity
The antibody reacts with human von Willebrand Factor/Factor VIII complex. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins. Polyclonal Rabbit Anti-Human Von Willebrand Factor reacts with von Willebrand Factor in endothelial cells, megakaryocytes and platelets when tested on formalin-fixed, paraffin-embedded normal human tissues (bone marrow, kidney, liver, lung, lymph node, skin and spleen) (7).
In crossed immunoelectrophoresis using 12.5 µL antibody per cm2 gel area against 10 µL of human plasma only one precipitate corresponding to the von Willebrand Factor appears. Staining: Coomassie Brilliant Blue.
As demonstrated by immunohistochemistry, the antibody cross-reacts with the vWF-equivalent protein on formalin-fixed, paraffin-embedded tissue in cow, horse, mouse and swine. Using other fixatives, cross reactivity was observed in chicken (8), dog and rat (9).
In crossed immunoelectrophoresis using 12.5 µL antibody per cm2 gel area against 10 µL of human plasma only one precipitate corresponding to the von Willebrand Factor appears. Staining: Coomassie Brilliant Blue.
As demonstrated by immunohistochemistry, the antibody cross-reacts with the vWF-equivalent protein on formalin-fixed, paraffin-embedded tissue in cow, horse, mouse and swine. Using other fixatives, cross reactivity was observed in chicken (8), dog and rat (9).
Reagent Provided
Purified immunoglobulin fraction of rabbit antiserum provided in liquid form. In 0.1 mol/L NaCL, 15 mmol/L NaN3.
Protein concentration g/L: See label on vial.
The titre variation between different lots of A0082 is less than 10%. This is achieved by adjusting the titre of each individual lot to match the titre of an antibody reference preparation kept at -80 °C.
Protein concentration g/L: See label on vial.
The titre variation between different lots of A0082 is less than 10%. This is achieved by adjusting the titre of each individual lot to match the titre of an antibody reference preparation kept at -80 °C.
References
- Weidner N, Semple JP, Welch WR, Folkman J. Tumor angiogenesis and metastasis - correlation in invasive breast carcinoma. New Engl J Med 1991;324:1-8.
- Makhlouf HR, Ishak KG, Goodman ZD. Epithelioid Hemangioendothelioma of the liver. A clinopathologic study of 137 cases. Cancer 1999;85:562-80.
- Ingerslev J. A Sensitive ELISA for von Willebrand factor (vWf:Ag). Scand J Clin Lab Invest 1987;47:143-149.
- Sehested M, Hou-Jensen K. Factor VIII-related antigen as an endothelial cell marker in benign and malignant diseases. Virchows Arch (Pathol Anat) 1981;391:217-25.
- Yablonka-Reuveni Z. The emergence of the endothelial cell lineage in the chick embryo can be detected by uptake of acetylated low density lipoprotein and the presence of a von Willebrand-like Factor. Dev. Biol. 1989;132:230-240.
- Roberta Schmidt. Evaluation of cross-species reactivity of antibodies to human antigens in animal models using immunoperoxidase techniques. J Histotechnol 1990;13:255-269.
| Product | Size |  | A0082 Ig fraction
| 0.2 mL Product No. A008229 |
|---|---|---|
| 2 mL Product No. A008202 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Human Von Willebrand Factor, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of acute myeloid leukemia FAB type M7 (1, 2) and angiosarcoma (3, 4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Polyclonal Rabbit Anti-Human Von Willebrand Factor, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of acute myeloid leukemia FAB type M7 (1, 2) and angiosarcoma (3, 4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Von Willebrand factor isolated from human plasma.
Specificity
The antibody reacts with human von Willebrand factor/factor VIII complex. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins. Polyclonal Rabbit Anti-Human Von Willebrand Factor reacts with von Willebrand factor in endothelial cells, megakaryocytes and platelets when tested on formalin-fixed, paraffin-embedded normal human tissues (bone marrow, kidney, liver, lung, lymph node, skin and spleen) (9).
In crossed immunoelectrophoresis using 12.5 µL concentrated antibody per cm2 gel area against 10 µL of human plasma only one precipitate corresponding to the von Willebrand factor appears. Staining: Coomassie Brilliant Blue
In crossed immunoelectrophoresis using 12.5 µL concentrated antibody per cm2 gel area against 10 µL of human plasma only one precipitate corresponding to the von Willebrand factor appears. Staining: Coomassie Brilliant Blue
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
References
- Pileri SA, Ascani S, Milani M, Visani G, Piccioli M, Fraternali G et al. Acute leukaemia immunophenotyping in bone-marrow routine sections. Br J Haematol 1999;105:394-401
- Manaloor EJ, Neiman RS, Heilman DK, Albitar M, Casey T, Vattuone T et al. Immunohistochemistry can be used to subtype acute myeloid leukemia in routinely processed bone marrow biopsy specimens. Comparison with flow cytometry. Am J Clin Pathol 2000;113:814-22
- Meis-Kindblom J, Kindblom LG. Angiosarcoma of soft tissue: A study of 80 cases. Am J Surg Pathol 1998;22:689-97
- Fanburg-Smith JC, Furlong MA, Childers ELB. Oral and salivary gland angiosarcoma: A clinicopathologic study of 29 cases. Mod Pahol 2003;16:263-71
- Sehested M, Hou-Jensen K. Factor VIII related antigen as an endothelial cell marker in benign andmalignant diseases. Virchows Arch (Pathol Anat) 1981;391:217-25.
| Product | Size | | IR527 RTU, FLEX
| 60 tests, 12 mL Product No. IR52761 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IR600) Positive tissue: Colon and liver | 120 tests, 24 mL Product No. IR60066 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Human Von Willebrand Factor, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of acute myeloid leukemia FAB type M7 (1, 2) and angiosarcoma (3, 4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Polyclonal Rabbit Anti-Human Von Willebrand Factor, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of acute myeloid leukemia FAB type M7 (1, 2) and angiosarcoma (3, 4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Von Willebrand factor isolated from human plasma.
Specificity
The antibody reacts with human von Willebrand factor/factor VIII complex. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins. Polyclonal Rabbit Anti-Human Von Willebrand Factor reacts with von Willebrand factor in endothelial cells, megakaryocytes and platelets when tested on formalin-fixed, paraffin-embedded normal human tissues (bone marrow, kidney, liver, lung, lymph node, skin and spleen) (9).
In crossed immunoelectrophoresis using 12.5 µL concentrated antibody per cm2 gel area against 10 µL of human plasma only one precipitate corresponding to the von Willebrand factor appears. Staining: Coomassie Brilliant Blue.
In crossed immunoelectrophoresis using 12.5 µL concentrated antibody per cm2 gel area against 10 µL of human plasma only one precipitate corresponding to the von Willebrand factor appears. Staining: Coomassie Brilliant Blue.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
References
- Pileri SA, Ascani S, Milani M, Visani G, Piccioli M, Fraternali G et al. Acute leukaemia immunophenotyping in bone-marrow routine sections. Br J Haematol 1999;105:394-401
- Manaloor EJ, Neiman RS, Heilman DK, Albitar M, Casey T, Vattuone T et al. Immunohistochemistry can be used to subtype acute myeloid leukemia in routinely processed bone marrow biopsy specimens. Comparison with flow cytometry. Am J Clin Pathol 2000;113:814-22
- Meis-Kindblom J, Kindblom LG. Angiosarcoma of soft tissue: A study of 80 cases. Am J Surg Pathol 1998;22:689-97
- Fanburg-Smith JC, Furlong MA, Childers ELB. Oral and salivary gland angiosarcoma: A clinicopathologic study of 29 cases. Mod Pahol 2003;16:263-71
- Sehested M, Hou-Jensen K. Factor VIII related antigen as an endothelial cell marker in benign andmalignant diseases. Virchows Arch (Pathol Anat) 1981;391:217-25.
| Product | Size | | IS527 RTU, FLEX
| 30 tests, 6 mL Product No. IS52730 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IS600) Positive tissue: Colon and liver | 60 tests, 12 mL Product No. IS60061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |