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Monoclonal Mouse Anti-Human
HER2-pY 1248, Phosphorylation Site Specific
Clone PN2A
The antibody labels HER2 protein that is phosphorylated at tyrosine residue 1248 and, therefore, activated. Overexpression of HER2 protein occurs in 10-40% of primary human invasive breast carcinomas (1). Of these, 12-58% have been reported to express HER2 in the activated tyrosine phosphorylated form (1-3). As the level of HER2 expression is not absolutely indicative of functional status, the detection of activated HER2 protein may be useful in the characterization of breast carcinomas (1).
Isotype IgG1, kappa
- Formalin
- HIER
References
- DiGiovanna MP, Carter D, Flynn SD, Stern DF. Functional assay for HER-2/neu demonstrates active signalling in a minority of HER-2/neu-overexpressing invasive human breast tumours. Br J Cancer 1996;74:802-6.
- Thor AD, Liu S, Edgerton S, Moore D 2nd, Kasowitz KM, Benz CC, et al. Activation (tyrosine phosphorylation) of ErbB-2 (HER-2/neu): a study of incidence and correlation with outcome in breast cancer. J Clin Oncol 2000;18:3230-9.
- DiGiovanna MP, Chu P, Davison TL, Howe CL, Carter D, Claus EB, et al. Active signalling by HER-2/neu in a subpopulation of HER-2/neu-overexpressing ductal carcinoma in situ: clinicopathological correlates. Cancer Res 2002;62:6667-73.
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human HER2-pY-1248, clone PN2A is intended for use in immunocytochemistry. The antibody labels activated human epidermal growth factor receptor-2 (HER2) that is phosphorylated at tyrosine residue 1248 (1). Identification of activated phosphorylated HER2 may be useful in the characterization of breast carcinomas (2, 3). Differential identification is aided by the results from a panel of antibodies. Inter-pretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human HER2-pY-1248, clone PN2A is intended for use in immunocytochemistry. The antibody labels activated human epidermal growth factor receptor-2 (HER2) that is phosphorylated at tyrosine residue 1248 (1). Identification of activated phosphorylated HER2 may be useful in the characterization of breast carcinomas (2, 3). Differential identification is aided by the results from a panel of antibodies. Inter-pretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Synthetic peptide, KTAENPE-pY-LGLDVPV, corresponding to the C-terminal 14 amino acids of HER2 plus an N-terminal lysine, coupled to keyhole limpet hemocyanin (1).
Specificity
In Western blotting of lysates of +/- epidermal growth factor stimulated SKBR3 human mammary carcinoma cells, the antibody recognized HER2 in a phosphorylation-dependent manner, whereas no labelling of phosphorylated HER1 was seen (1). No labelling was observed in Western blotting of different cell lysates containing different tyrosine phosphorylated proteins including HER4, platelet derived growth factor receptor, v-src and v-abl (1).
As demonstrated by immunocytochemistry, the antibody labels neoplastic cells in formalin-fixed, paraffin-embedded human breast carcinoma. This labelling could be blocked by a phospho-HER2-pY-1248 peptide (1).
As demonstrated by immunocytochemistry, the antibody cross-reacts with the phosphorylated rat HER2-equivalent protein overexpressed in transgenic mice (6).
As demonstrated by immunocytochemistry, the antibody labels neoplastic cells in formalin-fixed, paraffin-embedded human breast carcinoma. This labelling could be blocked by a phospho-HER2-pY-1248 peptide (1).
As demonstrated by immunocytochemistry, the antibody cross-reacts with the phosphorylated rat HER2-equivalent protein overexpressed in transgenic mice (6).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: PN2A (1). Isotype: IgG1, kappa.
Mouse IgG concentration: See label on vial.
Clone: PN2A (1). Isotype: IgG1, kappa.
Mouse IgG concentration: See label on vial.
References
- DiGiovanna MP, Stern DF. Activation state-specific monoclonal antibody detects tyrosine phosphorylated p185neu/erbB-2 in a subset of human breast tumors overexpressing this receptor. Cancer Res 1995;55:1946-55.
- DiGiovanna MP, Carter D, Flynn SD, Stern DF. Functional assay for HER-2/neu demonstrates active signalling in a minority of HER-2/neu-overexpressing invasive human breast tumours. Br J Cancer 1996;74:802-6.
- Thor AD, Liu S, Edgerton S, Moore II D, Kasowitz KM, Benz CC, et al. Activation (tyrosine phosphorylation) of ErbB-2 (HER-2/neu): a study of incidence and correlation with outcome in breast cancer. J Clin Oncol 2000;18:3230-9.
- DiGiovanna MP, Lerman MA, Coffey RJ, Muller WJ, Cardiff RD, Stern DF. Active signaling by Neu in transgenic mice. Oncogene 1998;17:1877-84.
| Product | Size |  | M7269 Culture supernatant
| 0.2 mL Product No. M726929 |
|---|