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Monoclonal Mouse Anti-Human
EGFR, Wild-Type
Clone DAK-H1-WT
Reacts with the N-terminal part of the extracellular domain of human epidermal growth factor receptor (EGFR). EGFR is a 170 kDa transmembrane receptor that is expressed by a variety of normal cells including fibroblasts and a wide range of epithelia. This antibody does not label the 2-7 truncated EGFR variant (EGFR-vIII).
Isotype IgG1
- Formalin
- HIER
References
- Jorissen RN, Walker F, Pouliot N, Garrett TP, Ward CW, Burgess AW. Epidermal growth factor receptor: mechanisms of activation and signaling [review]. Exp Cell Res 2003;284:31-53.
- Excellent specificity
- High lot to lot consistency
- For immunohistochemistry (IHC)
- Certified manufacturing facilities guarantee full quality control
Intended Use
For research use only. Not for use in diagnostic procedures.
Monoclonal Mouse Anti-Human Wild-Type EGFR, Clone DAK-H1-WT, is recommended for use in immunohistochemistry. The antibody labels the N-terminal part of the extracellular domain of human epidermal growth factor receptor (EGFR). The antibody does not label the Δ2-7 truncated variant of EGFR (EGFR-vIII).
Monoclonal Mouse Anti-Human Wild-Type EGFR, Clone DAK-H1-WT, is recommended for use in immunohistochemistry. The antibody labels the N-terminal part of the extracellular domain of human epidermal growth factor receptor (EGFR). The antibody does not label the Δ2-7 truncated variant of EGFR (EGFR-vIII).
Immunogen
Extracellular fragment of recombinant human EGFR oncoprotein corresponding to amino acid 30 - 198, including the leader sequence in the numbering.
Specificity
In Western blotting of recombinant proteins containing only the extracellular domain of human EGFR, EGFR-vIII, HER2, HER3 or HER4 protein, the antibody only labels the EGFR extracellular domain protein. In Western blotting of recombinant proteins containing only the intracellular domain of human EGFR, HER2, HER3 or HER4 protein, the antibody does not label any of the proteins.
As demonstrated by immunohistochemistry on formalin-fixed, paraffin-embedded CHO cells transfected with the genes encoding for EGFR, EGFR-vIII, HER2, HER3 or HER4, the antibody cross-reacts with the EGFR protein but not with EGFR-vIII or any of the HER2, HER3 and HER4 encoded proteins.
As demonstrated by immunohistochemistry on formalin-fixed, paraffin-embedded tissue, the antibody does not cross-react with the EGFR-equivalent protein in cow, dog, horse, mouse, rat, and swine.
As demonstrated by immunohistochemistry on formalin-fixed, paraffin-embedded CHO cells transfected with the genes encoding for EGFR, EGFR-vIII, HER2, HER3 or HER4, the antibody cross-reacts with the EGFR protein but not with EGFR-vIII or any of the HER2, HER3 and HER4 encoded proteins.
As demonstrated by immunohistochemistry on formalin-fixed, paraffin-embedded tissue, the antibody does not cross-react with the EGFR-equivalent protein in cow, dog, horse, mouse, rat, and swine.
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant (containing fetal bovine serum) dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 0.015 mol/L sodium azide.
Clone: DAK-H1-WT. Isotype: IgG1, kappa.
Mouse IgG concentration mg/L: See label on vial.
Clone: DAK-H1-WT. Isotype: IgG1, kappa.
Mouse IgG concentration mg/L: See label on vial.
| Product | Size |  | M7298 Culture supernatant
| 0.2 mL Product No. M729829 |
|---|
Synonym(s) for Antigen
WT-EGFR