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Monoclonal Mouse Anti-Human
CD138
Clone MI15
CD138, syndecan-1, is a transmembrane proteoglycan with a main cellular expression in stratified and simple epithelia.
Within the hemopoietic system, CD138 is mainly confined to late stages of B-cell differentiation. CD138 expression is reduced during malignant transformation of various epithelia, and CD138 is rapidly shed by myeloma cells entering into apoptosis, making CD138 a marker of viable myeloma cells. This antibody is useful for the identification of malignancies involving terminally differentiated plasma cells, and CD138 is associated with multiple myeloma. Anti-CD138 may also be useful for the subclassification of diffuse large B-cell lymphomas.
Myeloma (FFPE) stained with FLEX Anti-CD138, Code IR642/IS642.
Myeloma. The majority of the neoplastic cells show a strong and distinct membranous staining reaction.
Clinical application for IR642/IS642. For identification of malignancies involving terminally differentiated plasma cells and multiple myeloma, and for subclassification of diffuse large B-cell lymphoma.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
FLEX Monoclonal Mouse Anti-Human CD138, Clone MI15, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of malignancies involving terminally differentiated plasma cells, and it is associated with multiple myeloma (1, 2). Antibodies to CD138 may also be useful for the subclassification of diffuse large B-cell lymphoma (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
The epitope recognized by anti-CD138, MI15, is found within the ectodomain (extracellular part) of the CD138 core protein. Blocking experiments have revealed that the epitope recognized by MI15 seems to be partly overlapping the epitope recognized by another antibody to CD138, clone B-B4 (1, 5). Anti-CD138, MI15, reacts strongly with CD138 expressed by multiple myeloma-derived cell lines, such as U266 and XG-1 (9).
Clone: MI15. Isotype: IgG1, kappa.
- Wijdenes J, Clément C, Klein B, Dore J-M. New B-cell CD antigens. BC29: CD138 (syndecan-1) workshop panel report. In: Kishimoto T, Kikutani H, von dem Borne AEG, Goyert SM, Mason DY, Miyasaka M, et al., editors. Leucocyte typing VI. White cell differentiation antigens. Proceedings of the 6th International Workshop and Conference; 1996 Nov 10-14; Kobe, Japan. New York, London: Garland Publishing Inc.; 1997. p. 249-52.
- Costes V, Magen V, Legouffe E, Durand L, Baldet P, Rossi J-F, et al. The MI15 monoclonal antibody (anti-syndecan-1) is a reliable marker for quantifying plasma cells in paraffin-embedded bone marrow biopsy specimens. Hum Pathol 1999;30:1405-11.
- Colomo L, López-Guillermo A, Perales M, Rives S, Martínez A, Bosch F, et al. Clinical impact of the differentiation profile assessed by immunophenotyping in patients with diffuse large B-cell lymphoma. Blood 2003;101:78-84.
- Horvathova M, Gaillard JP, Liautard J, Duperray C, Lavabre-Bertrand T, Bourquard P, et al. B30.6. Identification of novel and specific antigens of human plasma cells by mAb. In: Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, et al., editors. Leucocyte typing V. White cell differentiation antigens. Proceedings of the 5th International Workshop and Conference; 1993 Nov 3-7; Boston, USA. Oxford, New York, Tokyo: Oxford University Press; 1995. p. 713-4.
| Product | Size |  | IR642 Clone MI15
| 60 tests, 12 mL Product No. IR64261 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Tonsil | 120 tests, 24 mL Product No. IR75066 |
| Visualization System | EnVision™ FLEX+ (Code K8002) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800221 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | Silanized Slides (Code S3003) Recommended for greater adherence Alternative is FLEX IHC Microscope Slides (Code K8020) | 100 slides Product No. S300330 |