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Monoclonal Mouse Anti-Human
Nucleophosmin
Clone 376
Reacts with nucleophosmin (NPM) and its mutated counterpart NPMc. NPM is predominantly localized in the nucleus of cells in most tissues.
However, NPMc is aberrantly accumulated in the cytoplasm of leukemic blasts in a large subgroup of acute myeloid leukemia (AML) cases with a normal karyotype (1-2). In contrast nuclear and cytoplasmic labeling of leukemic blast cells is only observed in ~10% of AML cases with an abnormal karyotype, and none of these cases represent specific genetic abnormalities commonly observed in primary AML (1). Immunohistochemical detection of the NPMc phenotype is a tool for diagnosing AML carrying NPM1 exon 12 mutations and it may represent a simple and valuable indicator for lack of recurrent chromosomal abnormalities in primary AML (3).
- Falini B, Mecucci C, Tiacci E, Alcaly M, Rosatin R, Pasqualucci L, et al. Cytoplasmic nucleophosmin in acute myologenous leukemia with a normal karyotype. New Engl J Med 2005;352:254-66.
- Falini B, Bolli N, Shan J, Martelli PM, Liso A, Pucciarini A, et al. Both carboxy-terminus NES motif and mutated tryptophan(s) are crucial for aberrant nuclear export of nucleophosmin leukemic mutants in NPMc+ AML. Blood 2006;107:4514-23.
- Pasqualucci L, Liso A, Martelli MP, Bolli N, Pacini R, Tabarrinni A, et al. Mutated nucleophosmin detects clonal multilineage involvement in acute myeloid leukemia: Impact on WHO classification. Blood 2006: Epub. ahead of print.
Acute myeloid leukemia (FFPE) stained with FLEX Anti-Nucleophosmin, Code IR652/IS652.
Acute myeloid leukemia (NPM1 Exon 12 mutant; NPMc+). The majority of the leukemic blasts show a distinct nuclear staining reaction and a moderate to strong cytoplasmic staining reaction.
Clinical application for IR652/IS652 For identification of both wild-type nucleophosmin (NPM) and the mutated counterpart NPMc+.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
FLEX Monoclonal Mouse Anti-Human Nucleophosmin, Clone 376, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of both wild-type nucleophosmin (NPM) and the mutated counterpart NPMc+. Nucleophosmin is localized in the nuclei of normal cells in multiple tissues, but is aberrantly accumulated in the cytoplasm of leukemic blasts in a large subgroup of acute myeloid leukemia cases with a normal karyotype (1-3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Clone: 376. Isotype: IgG1, lambda.
- Grisendi S, Mecucci C, Falini B, Pandolfi PP. Nucleophosmin and cancer. Nature Rev Cancer 2006;6:493-505.
- Falini B, Mecucci C, Tiacci E, Alcaly M, Rosatin R, Pasqualucci L, et al. Cytoplasmic nucleophosmin in acute myologenous leukemia with a normal karyotype. New Engl J Med 2005;352:254-66.
- Falini B, Bolli N, Shan J, Martelli PM, Liso A, Pucciarini A, et al. Both carboxy-terminus NES motif and mutated tryptophan(s) are crucial for aberrant nuclear export of nucleophosmin leukemic mutants in NPMc+ AML. Blood 2006;107:4514-23.
| Product | Size |  | IR652 Clone 376
| 60 tests, 12 mL Product No. IR65261 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Liver | 120 tests, 24 mL Product No. IR75066 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |