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Monoclonal Mouse Anti-Human
CD68
Clone KP1
Labels human monocytes, macrophages and myeloid cells.
It is of value for demonstrating reactive macrophages in a wide variety of normal and pathological specimens and for the immunophenotyping of myeloid and histiocytic cells and their neoplastic equivalents.
Acute myeloid leukemia (FFPE) stained with FLEX Anti-CD68, Code IR609/IS609.
Acute myeloid leukemia (FAB type M5). The neoplastic cells show a distinct granular cytoplasmic staining reaction.
Clinical application for IR609/IS609. For identification of macrophages, other members of the mononuclear phagocyte lineage, and neoplasm of myeloid and macrophage/monocyte origin.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human CD68, Clone KP1, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels macrophages and other members of the mononuclear phagocyte lineage and is useful for the identification of neoplasms of myeloid and macrophage/monocyte origin (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human CD68, Clone KP1, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels macrophages and other members of the mononuclear phagocyte lineage and is useful for the identification of neoplasms of myeloid and macrophage/monocyte origin (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Lysosomal fraction of human lung macrophages (4).
Specificity
Anti-Human CD68, clone KP1, was clustered as anti-CD68 at the Fourth International Workshop and Conference on Human Leucocyte Differentiation Antigens (5).
In Western blotting of extracts of lung, spleen and U937 cells, diffuse 110, 70 and 40 kDa bands were detected when using reducing conditions. Under non-reducing conditions the spleen extract showed an additional 220 kDa band (4).
SDS-PAGE analysis of immunoprecipitates formed between the antibody and 125I-labeled lysates from human spleen with B-cell lymphoma rich in macrophages shows reaction with a 110 kDa polypeptide, corresponding to CD68 (4).
In Western blotting of extracts of lung, spleen and U937 cells, diffuse 110, 70 and 40 kDa bands were detected when using reducing conditions. Under non-reducing conditions the spleen extract showed an additional 220 kDa band (4).
SDS-PAGE analysis of immunoprecipitates formed between the antibody and 125I-labeled lysates from human spleen with B-cell lymphoma rich in macrophages shows reaction with a 110 kDa polypeptide, corresponding to CD68 (4).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: KP1 (4). Isotype: IgG1, kappa.
Clone: KP1 (4). Isotype: IgG1, kappa.
References
- Warnke RA, Pulford KAF, Pallesen G, Ralfkiaer E, Brown DC, Gatter KC, et al. Diagnosis of myelomonocytic and macrophage neoplasms in routinely processed tissue biopsies with monoclonal antibody KP1. Am J Pathol 1989;135:1089-95.
- Pulford KAF, Rigney EM, Micklem KJ, Jones M, Stross WP, Gatter KC, et al. KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol 1989;42:414-21.
- Micklem K, Cordell J, Rigney E, Simmons D, Pulford K, Stross P, et al. M13.1. A macrophage-associated antigen defined by five mAB. In: Knapp W, Dörken B, Gilks WR, Rieber EP, Schmidt RE, Stein H, et al., editors. Leucocyte typing IV. White cell differentiation antigens. Proceedings of the 4th International Workshop and Conference; 1989 Feb 21-25; Vienna, Austria. Oxford, New York, Tokyo: Oxford University Press; 1989. p. 843-46.
| Product | Size |  | IS609 Clone KP1
| 30 tests, 6 mL Product No. IS60930 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Tonsil and brain | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |