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Polyclonal Rabbit Anti-Human
IgA, Specific for Alpha-Chains
The antigen used for immunization is serum IgA. The very high specificity and the good performance of the antibody have been ascertained in immunohistochemistry as well as in indirect ELISA and immunoblotting.
Additionally, the specificity has been tested by crossed immunoelectrophoresis using 12.5 microlitre antibody per square cm gel area against 2 microlitre human plasma.
- Frozen
- Formalin
- Enzyme/HIER
Tonsil (FFPE) stained with FLEX Anti-IgA, Code IR510/IS510.
Tonsil. Plasma cells and immunoblasts show a moderate to strong cytoplasmic staining reaction.
Clinical application for IR510/IS510. For identification of plasma cells and related lymphoid cells containing IgA, and for the classification of B-cell neoplasias.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Polyclonal Rabbit Anti-Human IgA, Code A0262, is intended for use in immunohistochemistry. The antibody is also well suited for ELISA and immunoblotting (1).
In immunohistochemistry, the antibody labels plasma cells and related lymphoid cells containing IgA, and it is a useful tool for the classification of patients with B-cell neoplasias (2-4). Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of plasma cells (2, 5). Differential identification is aided by the results from a panel of antibodies.Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Polyclonal Rabbit Anti-Human IgA, Code A0262, is intended for use in immunohistochemistry. The antibody is also well suited for ELISA and immunoblotting (1).
In immunohistochemistry, the antibody labels plasma cells and related lymphoid cells containing IgA, and it is a useful tool for the classification of patients with B-cell neoplasias (2-4). Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of plasma cells (2, 5). Differential identification is aided by the results from a panel of antibodies.Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Immunogen
IgA isolated from a pool of normal human sera.
Specificity
The antibody reacts with the alpha-chains of human IgA. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins.
The specificity of the antibody has been ascertained as follows:
Crossed immunoelectrophoresis: Only the IgA immunoprecipitation arch appears when using 12.5 µL antibody cm2 gel area against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
ELISA: No significant reaction is seen in indirect ELISA using human IgG and IgM as coating antigens. In double antibody sandwich ELISA no significant reaction is observed with human plasma stripped of IgA.
Cross-reaction with IgA from other species may occur. As demonstrated by rocket immunoelectrophoresis, the antibody cross-reacts with the IgA-equivalent protein in cow, deer, goat, horse, mink, mouse, polecat, sheep and swine (9).
The specificity of the antibody has been ascertained as follows:
Crossed immunoelectrophoresis: Only the IgA immunoprecipitation arch appears when using 12.5 µL antibody cm2 gel area against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
ELISA: No significant reaction is seen in indirect ELISA using human IgG and IgM as coating antigens. In double antibody sandwich ELISA no significant reaction is observed with human plasma stripped of IgA.
Cross-reaction with IgA from other species may occur. As demonstrated by rocket immunoelectrophoresis, the antibody cross-reacts with the IgA-equivalent protein in cow, deer, goat, horse, mink, mouse, polecat, sheep and swine (9).
Reagent Provided
Purified immunoglobulin fraction of rabbit antiserum provided in liquid form. In 0.1 mol/L NaCl, 15 mmol/L NaN3.
Protein concentration g/L: See label on vial. Antibody titre (SRI): 2200 mg/L (8).
The titre variation between different lots of A0262 is less than 10%. This is achieved by adjusting the titre of each individual lot to match the titre of an antibody reference preparation kept at -80 °C.
Protein concentration g/L: See label on vial. Antibody titre (SRI): 2200 mg/L (8).
The titre variation between different lots of A0262 is less than 10%. This is achieved by adjusting the titre of each individual lot to match the titre of an antibody reference preparation kept at -80 °C.
References
- Withold W, Reinauer H. An immunoblotting procedure following agarose gel electrophoresis for detection of Bence Jones proteinuria compared with immunofixation and quantitative light chain determination. Eur J Clin Chem Clin Biochem 1995;33:135-8.
- Taylor CR, Burns J. The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody. J Clin Pathol 1974;27:14-20.
- Taylor CR, Mason DY. The immunohistological detection of intracellular immunoglobulin in formalin-paraffin sections from multiple myeloma and related conditions using the immunoperoxidase technique. Clin exp Immunol 1974;18:417-29.
- Taylor CR, Russell R, Chandor S. An immunohistologic study of multiple myeloma and related conditions, using an immunoperoxidase method. Am J Clin Pathol 1978;70:612-22.
- Beschorner R, Horny H-P, Petrusch UR, Kaiserling E. Frequent expression of haemopoietic and non-haemopoietic antigens by reactive plasma cells: an immunohistochemical study using formalin-fixed, paraffin-embedded tissue. Histol Histopathol 1999;14:805-12.
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| Product | Size |  | A0262 Ig fraction
| 1 mL Product No. A026201-2 | | F0204 FITC. Ig fraction
| 2 mL Product No. F020402-2 | | F0316 FITC. F(ab')2
| 1 mL Product No. F031601 |
|---|
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Human IgA, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of plasma cells and related lymphoid cells containing IgA, and it is a useful tool for the classification of patients with B-cell neoplasias (1-3). Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of plasma cells (1, 4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Polyclonal Rabbit Anti-Human IgA, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of plasma cells and related lymphoid cells containing IgA, and it is a useful tool for the classification of patients with B-cell neoplasias (1-3). Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of plasma cells (1, 4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
IgA isolated from a pool of normal human sera.
Specificity
The antibody reacts with the alpha-chains of human IgA. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins.
The specificity of the antibody has been ascertained as follows:
Crossed immunoelectrophoresis: Only the IgA immunoprecipitation arch appears when using 12.5 µL concentrated antibody cm2 gel area against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
ELISA: No significant reaction is seen in indirect ELISA using human IgG and IgM as coating antigens. In double antibody sandwich ELISA no significant reaction is observed with human plasma stripped of IgA.
The specificity of the antibody has been ascertained as follows:
Crossed immunoelectrophoresis: Only the IgA immunoprecipitation arch appears when using 12.5 µL concentrated antibody cm2 gel area against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
ELISA: No significant reaction is seen in indirect ELISA using human IgG and IgM as coating antigens. In double antibody sandwich ELISA no significant reaction is observed with human plasma stripped of IgA.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
References
- Taylor CR, Burns J. The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labeled antibody. J Clin Pathol 1974;27:14-20.
- Taylor CR, Mason DY. The immunohistological detection of intracellular immunoglobulin in formalin-paraffin sections from multiple myeloma and related conditions using the immunoperoxidase technique. Clin exp Immunol 1974;18:417-29.
- Taylor CR, Russell R, Chandor S. An immunohistologic study of multiple myeloma and related conditions, using an immunoperoxidase method. Am J Clin Pathol 1978;70:612-22.
- Beschorner R, Horny H-P, Petrusch UR, Kaiserling E. Frequent expression of haemopoietic and non-haemopoietic antigens by reactive plasma cells: an immunohistochemical study using formalin-fixed, paraffin-embedded tissue. Histol Histopathol 1999;14:805-12.
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| Product | Size | | IR510 RTU, FLEX
| 60 tests, 12 mL Product No. IR51061-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IR600) Positive tissue: Tonsil | 120 tests, 24 mL Product No. IR60066-2 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021-2 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Human IgA, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of plasma cells and related lymphoid cells containing IgA, and it is a useful tool for the classification of patients with B-cell neoplasias (1-3). Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of plasma cells (1, 4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Polyclonal Rabbit Anti-Human IgA, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of plasma cells and related lymphoid cells containing IgA, and it is a useful tool for the classification of patients with B-cell neoplasias (1-3). Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of plasma cells (1, 4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
IgA isolated from a pool of normal human sera.
Specificity
The antibody reacts with the alpha-chains of human IgA. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins.
The specificity of the antibody has been ascertained as follows:
Crossed immunoelectrophoresis: Only the IgA immunoprecipitation arch appears when using 12.5 µL concentrated antibody per square cm gel area against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
ELISA: No significant reaction is seen in indirect ELISA using human IgG and IgM as coating antigens. In double antibody sandwich ELISA no significant reaction is observed with human plasma stripped of IgA.
The specificity of the antibody has been ascertained as follows:
Crossed immunoelectrophoresis: Only the IgA immunoprecipitation arch appears when using 12.5 µL concentrated antibody per square cm gel area against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
ELISA: No significant reaction is seen in indirect ELISA using human IgG and IgM as coating antigens. In double antibody sandwich ELISA no significant reaction is observed with human plasma stripped of IgA.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
References
- Taylor CR, Burns J. The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labeled antibody. J Clin Pathol 1974;27:14-20.
- Taylor CR, Mason DY. The immunohistological detection of intracellular immunoglobulin in formalin-paraffin sections from multiple myeloma and related conditions using the immunoperoxidase technique. Clin exp Immunol 1974;18:417-29.
- Taylor CR, Russell R, Chandor S. An immunohistologic study of multiple myeloma and related conditions, using an immunoperoxidase method. Am J Clin Pathol 1978;70:612-22.
- Beschorner R, Horny H-P, Petrusch UR, Kaiserling E. Frequent expression of haemopoietic and non-haemopoietic antigens by reactive plasma cells: an immunohistochemical study using formalin-fixed, paraffin-embedded tissue. Histol Histopathol 1999;14:805-12.
Check Your Price & Order Online
| Product | Size | | IS510 RTU, FLEX
| 30 tests, 6 mL Product No. IS51030-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IS600) Positive tissue: Tonsil | 60 tests, 12 mL Product No. IS60061-2 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021-2 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |