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Monoclonal Mouse Anti-Human
BCL6 Protein
Clone PG-B6p
The BCL6 gene encodes a 706 amino acid nuclear protein of the Kruppel-type zinc finger protein.
It is rearranged in about 30% of diffuse large B-cell lymphomas, and is expressed predominantly in normal germinal centre B cells and related lymphomas (1). The antibody gives a strong nuclear labeling of BCL6 protein in follicular lymphomas, diffuse large B-cell lymphomas, Burkitt's lymphomas, and nodular, lymphocyte-predominance Hodgkin's disease. The PG-B6p antibody is useful for differentiating proliferation centres in B-CLL (BCL2+/BCL6-) from trapped germinal centres (BCL2-/BCL6+) in mantle cell lymphomas, and for identifying neoplastic cells in cases of nodular, lymphocyte-predominance Hodgkin's disease. BCL6 protein is not expressed in B-CLL, hairy cell leukemia, mantle cell and marginal-zone derived lymphomas (2, 3).
Isotype IgG1, kappa
- Frozen
- Formalin
- HIER
References
- Offit K, Lo Coco F, Louie DC, Parsa NZ, Leung D, Portlock C, et al. Rearrangement of the bcl6 gene as a prognostic marker in diffuse large-cell lymphoma. N Engl J Med 1994;331:74-80.
- Flenghi L, Bigerna B, Fizzotti M, Venturi S, Pasqualucci L, Pileri S, et al. Monoclonal antibodies PG-B6a and PG-B6p recognize, respectively, a highly conserved and a formol-resistant epitope on the human BCL-6 protein amino-terminal region. Am J Pathol 1996;148:1543-55.
- Falini B, Bigerna B, Pasqualucci L, Fizzotti M, Martelli MF, Pileri S, et al. Distinctive expression pattern of the BCL-6 protein in nodular lymphocyte predominance Hodgkin's disease. Blood 1996;87:465-71.
Diffuse large B-cell lymphoma (germinal center subtype) (FFPE) stained with FLEX Anti-BCL6 Protein, Code IR625/IS625.
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Diffuse large B-cell lymphoma (germinal center subtype). The majority of the neoplastic cells show a distinct nuclear staining reaction.
Clinical application for IR625/IS625. For classification of diffuse large B-cell lymphomas, follicular lymphomas and Burkitt lymphoma/leukemia.
Follicular lymphoma. The majority of the neoplastic cells show a distinct nuclear staining reaction.
Clinical application for IR625/IS625. For classification of diffuse large B-cell lymphomas, follicular lymphomas and Burkitt lymphoma/leukemia.
Tonsil. The majority of the germinal center B cells show a moderate to strong nuclear staining reaction.
Recommended control for IR625/IS625. Tonsil.
Tonsil. The squamous epithelial cells show a weak to moderate nuclear staining reaction.
Recommended control for IR625/IS625. Tonsil.
Primary mediastinal B-cell lymphoma stained with Anti-BCL6 Protein.
Clinical application for IR625/IS625. For classification of diffuse large B-cell lymphomas, follicular lymphomas and Burkitt lymphoma/leukemia.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human BCL6 Protein, Clone PG-B6p, is intended for use in immunohistochemistry. The antibody labels cells expressing BCL6 protein and is a useful tool for differentiating proliferating centres in B-cell chronic lymphocytic lymphomas (BCL2+/BCL6-) from trapped germinal centres in mantle cell lymphomas (BCL2-/BCL6+), and for identifying neoplastic cells in cases of nodular, lymphocyte-predominance Hodgkin's disease (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human BCL6 Protein, Clone PG-B6p, is intended for use in immunohistochemistry. The antibody labels cells expressing BCL6 protein and is a useful tool for differentiating proliferating centres in B-cell chronic lymphocytic lymphomas (BCL2+/BCL6-) from trapped germinal centres in mantle cell lymphomas (BCL2-/BCL6+), and for identifying neoplastic cells in cases of nodular, lymphocyte-predominance Hodgkin's disease (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Recombinant BCL6 protein corresponding to amino acids 3-484 of the human BCL6 protein (1).
Specificity
SDS-PAGE analysis of immunoprecipitates formed between the antibody and total lysates of Bjab cells (BCL6+) and BCL6 transfected EB3 cells, shows reaction with a ~95 kDa fragment corresponding to BCL6. No reaction is observed with Rd cells (BCL6-) and EB3 cells transfected with a control plasmid (1).
In Western blotting of total lysates of Bjab cells and BCL6 transfected EB3 cells, or control transfected EB3 cells, the antibody did not label any bands, suggesting lack of reactivity with denatured BCL6 protein (1).
As demonstrated by immunohistochemistry, the antibody cross-reacts with the BCL6-equivalent protein in various mammals, including cow, rabbit, rat, sheep, and swine. No cross-reactivity was observed in chicken and pigeon (1).
In Western blotting of total lysates of Bjab cells and BCL6 transfected EB3 cells, or control transfected EB3 cells, the antibody did not label any bands, suggesting lack of reactivity with denatured BCL6 protein (1).
As demonstrated by immunohistochemistry, the antibody cross-reacts with the BCL6-equivalent protein in various mammals, including cow, rabbit, rat, sheep, and swine. No cross-reactivity was observed in chicken and pigeon (1).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: PG-B6p (1). Isotype: IgG1, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
Clone: PG-B6p (1). Isotype: IgG1, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
References
- Flenghi L, Bigerna B, Fizzotti M, Venturi S, Pasqualucci L, Pileri S, et al. Monoclonal antibodies PG-B6a and PG-B6p recognize, respectively, a highly conserved and a formol-resistant epitope on the human BCL-6 protein amino-terminal region. Am J Pathol 1996;148:1543-55.
| Product | Size |  | M7211 Culture supernatant
| 0.2 mL Product No. M721129 |
|---|---|---|
| 1 mL Product No. M721101 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human BCL6 Protein, Clone PG-B6p, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for classification of diffuse large B-cell lymphomas, follicular lymphomas and Burkitt's lymphomas (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human BCL6 Protein, Clone PG-B6p, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for classification of diffuse large B-cell lymphomas, follicular lymphomas and Burkitt's lymphomas (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Recombinant BCL6 protein corresponding to amino acids 3-484 of the human BCL6 protein (1).
Specificity
In Western blotting of total lysates of Bjab cells and BCL6 transfected EB3 cells, or control transfected EB3 cells, the antibody did not label any bands, suggesting lack of reactivity with denatured BCL6 protein (1).
SDS-PAGE analysis of immunoprecipitates formed between total lysates of Bjab cells (BCL6+) and BCL6 transfected EB3 cells and the antibody, shows reaction with a ~95 kDa fragment corresponding to BCL6. No reaction is observed with Rd cells (BCL6-) and EB3 cells transfected with a control plasmid (1).
SDS-PAGE analysis of immunoprecipitates formed between total lysates of Bjab cells (BCL6+) and BCL6 transfected EB3 cells and the antibody, shows reaction with a ~95 kDa fragment corresponding to BCL6. No reaction is observed with Rd cells (BCL6-) and EB3 cells transfected with a control plasmid (1).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: PG-B6p (1). Isotype: IgG1, kappa.
Clone: PG-B6p (1). Isotype: IgG1, kappa.
References
- Flenghi L, Bigerna B, Fizzotti M, Venturi S, Pasqualucci L, Pileri S, et al. Monoclonal antibodies PG-B6a and PG-B6p recognize, respectively, a highly conserved and a formol-resistant epitope on the human BCL-6 protein amino-terminal region. Am J Pathol 1996;148:1543-55.
| Product | Size | | IR625 RTU, FLEX
| 60 tests, 12 mL Product No. IR62561 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Tonsil | 120 tests, 24 mL Product No. IR75066 |
| Visualization System | EnVision™ FLEX+ (Code K8002) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800221 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | Silanized Slides (Code S3003) Recommended for greater adherence Alternative is FLEX IHC Microscope Slides (Code K8020) | 100 slides Product No. S300330 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human BCL6 Protein, Clone PG-B6p, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for classification of diffuse large B-cell lymphomas, follicular lymphomas and Burkitt's lymphomas (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human BCL6 Protein, Clone PG-B6p, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for classification of diffuse large B-cell lymphomas, follicular lymphomas and Burkitt's lymphomas (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Recombinant BCL6 protein corresponding to amino acids 3-484 of the human BCL6 protein (1).
Specificity
In Western blotting of total lysates of Bjab cells and BCL6 transfected EB3 cells, or control transfected EB3 cells, the antibody did not label any bands, suggesting lack of reactivity with denatured BCL6 protein (1).
SDS-PAGE analysis of immunoprecipitates formed between total lysates of Bjab cells (BCL6+) and BCL6 transfected EB3 cells and the antibody, shows reaction with a ~95 kDa fragment corresponding to BCL6. No reaction is observed with Rd cells (BCL6-) and EB3 cells transfected with a control plasmid (1).
SDS-PAGE analysis of immunoprecipitates formed between total lysates of Bjab cells (BCL6+) and BCL6 transfected EB3 cells and the antibody, shows reaction with a ~95 kDa fragment corresponding to BCL6. No reaction is observed with Rd cells (BCL6-) and EB3 cells transfected with a control plasmid (1).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: PG-B6p (1). Isotype: IgG1, kappa.
Clone: PG-B6p (1). Isotype: IgG1, kappa.
References
- Flenghi L, Bigerna B, Fizzotti M, Venturi S, Pasqualucci L, Pileri S, et al. Monoclonal antibodies PG-B6a and PG-B6p recognize, respectively, a highly conserved and a formol-resistant epitope on the human BCL-6 protein amino-terminal region. Am J Pathol 1996; 148:1543-55.
| Product | Size | | IS625 RTU, FLEX
| 30 tests, 6 mL Product No. IS62530 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Tonsil | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX+ (Code K8012) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801221 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | Silanized Slides (Code S3003) Recommended for greater adherence Alternative is FLEX IHC Microscope Slides (Code K8020) | 100 slides Product No. S300330 |