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Monoclonal Mouse Anti-Human
CD68
Clone EBM11
Labels human monocytes and macrophages and can be used for identifying a population of cells as being of mononuclear phagocyte origin, for demonstrating the macrophage origin of giant cells, and for studying the number of macrophages infiltrating a neoplasm.
Isotype IgG1, kappa
- Frozen
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human CD68, Clone EBM11, is recommended for use in immunohistochemistry. The antibody labels CD68. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human CD68, Clone EBM11, is recommended for use in immunohistochemistry. The antibody labels CD68. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Macrophages isolated from human lung.
Specificity
The antibody was clustered as anti-CD68 at the Fourth International Workshop and Conference on Human Leucocyte Differentiation Antigens (1).
SDS-PAGE analysis of immunoprecipitates formed between spleen cell lysate and the antibody shows reaction with 110 kDa band, corresponding to CD68 (2).
As demonstrated by immunohistochemistry the antibody cross-reacts with the CD68-equivalent protein in Rhesus monkey (3) and cattle (4).
SDS-PAGE analysis of immunoprecipitates formed between spleen cell lysate and the antibody shows reaction with 110 kDa band, corresponding to CD68 (2).
As demonstrated by immunohistochemistry the antibody cross-reacts with the CD68-equivalent protein in Rhesus monkey (3) and cattle (4).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialyzed against 50 mmol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: EBM11. Isotype: IgG1, kappa.
Mouse IgG concentration (mg/L): See label on vial.
Clone: EBM11. Isotype: IgG1, kappa.
Mouse IgG concentration (mg/L): See label on vial.
References
- Stockinger H. M13. Cluster report: CD68. In: Knapp W, Dörken B, Gilks WR, Rieber EP, Schmidt RE, Stein H, et al., editors. Leucocyte typing IV. White cell differentiation antigens. Proceedings of the 4th International Workshop and Conference; 1989 Feb 21-25; Vienna, Austria. Oxford, New York, Tokyo: Oxford University Press; 1989. p 841-3.
- Micklem K, Cordell J, Rigney E, Simmons D, Pulford K et al. M13.1. A macrophage-associated antigen defined by five mAb. In: Knapp W, Dörken B, Gilks WR, Rieber EP, Schmidt RE, Stein H, et al., editors. Leucocyte typing IV. White cell differentiation antigens. Proceedings of the 4th International Workshop and Conference; 1989 Feb 21-25; Vienna, Austria. Oxford, New York, Tokyo: Oxford University Press; 1989. p 843-6.
- Ringler DJ, Walsh DG, MacKey JJ, Hunt RD, King NW. Immunophenotypic characterization of mononuclear phagocytes and dendritic cells in lymphoid organs of the rhesus monkey. Clin Immunol Immunopathol 1988;49:349-64.
- Bielefeldt-Ohmann H, Sabara M, Lawman MJP, Griebel P, Babiuk LA. A monoclonal antibody detects macrophage maturation antigen which appears independently of Class II antigen expression. Reactivity of monoclonal EBM11 with bovine macrophages. J Immunol 1988;140:2201-9.
| Product | Size |  | M0718 Culture supernatant
| 1 mL Product No. M071801 |
|---|