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Monoclonal Mouse Anti-Human
CD79α
Clone JCB117
CD79α is encoded by the mb-1 gene and was previously called Igα.
The antibody recognizes an extracellular epitope expressed on the CD79α molecule, and detects the full spectrum of B-cell neoplasms in routine biopsy material. In addition to the expression in B cells, CD79α has been found to be co-expressed with CD3 in 10% of cases of T-lymphoblastic leukemia/lymphoma. Antibodies to CD79α may also be useful in the differential diagnosis of Hodgkin's disease.
The antibody is well-suited for use on cell smears and tissue sections, but not for flow cytometry.
Isotype IgG1, kappa
- Frozen
- Formalin
- HIER
References
- Mason DY, Cordell JL, Brown MH, Borst J, Jones M, Pulford K, et al. CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood 1995;86:1453-9.
- Pilozzi E, Pulford K, Jones M, Muller-Hermelink HK, Falini B, Ralfkiaer E, et al. Co-expression of CD79a (JCB117) and CD3 by lymphoblastic lymphoma. J Pathol 1998;186:140-3.
- Chu PG, Arber DA. CD79: a review. Appl Immunohistochem Mol Morphol 2001;9:97-106
Precursor B-lymphoblastic leukemia/lymphoma (FFPE) stained with FLEX Anti-CD79α, Code IR621/IS621.
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Precursor B-lymphoblastic leukemia/lymphoma disseminated to the testis. The majority of the neoplastic cells show a weak to moderate staining reaction
Clinical application for IR621/IS621. For identification of B cells during all stages of differentiation and of B-cell neoplasm.
Plasmacytoma. The majority of the neoplastic cells show a moderate to strong cytoplasmic staining reaction.
Clinical application for IR621/IS621. For identification of B cells during all stages of differentiation and of B-cell neoplasm.
Tonsil. The mantle zone B cells show a strong staining reaction, while the germinal center B cells show a moderate staining reaction. Plasma cells show a strong cytoplasmic staining reaction.
Recommended control for IR621/IS621. Tonsil.
Tonsil stained with Anti-CD79α.
Recommended control for IR621/IS621. Tonsil.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human CD79α, Clone JCB117, is intended for use in immunohistochemistry. The antibody labels B cells and is a useful tool for the identification of B-cell neoplasms of all maturation stages (1). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human CD79α, Clone JCB117, is intended for use in immunohistochemistry. The antibody labels B cells and is a useful tool for the identification of B-cell neoplasms of all maturation stages (1). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Recombinant protein containing part of the extracellular portion of the human CD79α glycoprotein (1).
Specificity
The antibody was clustered as anti-CD79α at the Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens held in Kobe in 1996 (4).
In Western blotting of Ramos B-cell lysate, the antibody labels a band corresponding to CD79α under reducing conditions, and a band corresponding to CD79αβ under non-reducing conditions (1).
SDS-PAGE analysis of immunoprecipitates formed between the antibody and 125I-labeled, mildly denatured, and reduced lysate of Ramos B cells, shows weak reaction with a 43 kDa polypeptide corresponding to CD79α.
The antibody was tested by flow cytometry against normal peripheral B-cell lines, and against mature and immature B-cell lines (Ramos, Raji, and NALM-1), and was consistently negative, indicating that the epitope recognized is not present, or is not accessible, on living cells. However, the antibody specifically labels B cells in paraffin-embedded tissue sections (4).
In Western blotting of Ramos B-cell lysate, the antibody labels a band corresponding to CD79α under reducing conditions, and a band corresponding to CD79αβ under non-reducing conditions (1).
SDS-PAGE analysis of immunoprecipitates formed between the antibody and 125I-labeled, mildly denatured, and reduced lysate of Ramos B cells, shows weak reaction with a 43 kDa polypeptide corresponding to CD79α.
The antibody was tested by flow cytometry against normal peripheral B-cell lines, and against mature and immature B-cell lines (Ramos, Raji, and NALM-1), and was consistently negative, indicating that the epitope recognized is not present, or is not accessible, on living cells. However, the antibody specifically labels B cells in paraffin-embedded tissue sections (4).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: JCB117 (1). Isotype: IgG1, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
Clone: JCB117 (1). Isotype: IgG1, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
References
- Mason DY, Cordell JL, Brown MH, Borst J, Jones M, Pulford K, et al. CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood 1995;86:1453-9.
- Nakamura T. BC20. CD79 workshop panel report. In: Kishimoto T, Kikutani H, von dem Borne AEG, Goyert SM, Mason DY, Miyasaka M, et al., editors. Leucocyte typing VI. White cell differentiation antigens. Proceedings of the 6th International Workshop and Conference; 1996 Nov 10-14; Kobe, Japan. New York, London: Garland Publishing Inc.; 1997. p. 180-2.
| Product | Size |  | M7050 Culture supernatant
| 0.2 mL Product No. M705029 |
|---|---|---|
| 1 mL Product No. M705001 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human CD79α, Clone JCB117, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels B cells and is useful for the identification of B-cell neoplasms of all maturation stages (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human CD79α, Clone JCB117, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels B cells and is useful for the identification of B-cell neoplasms of all maturation stages (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Recombinant protein containing part of the extracellular portion of the human CD79α glycoprotein (1).
Specificity
Anti-Human CD79α, clone JCB117, was clustered as anti-CD79α at the Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens (4).
In Western blotting of Ramos B-cell lysate, the antibody labels a band corresponding to CD79α under reducing conditions, and a band corresponding to CD79αβ under non-reducing conditions (1).
SDS-PAGE analysis of immunoprecipitates formed between the antibody and 125I-labeled, mildly denatured, and reduced lysate of Ramos B cells, shows weak reaction with a 43 kDa polypeptide corresponding to CD79α.
In Western blotting of Ramos B-cell lysate, the antibody labels a band corresponding to CD79α under reducing conditions, and a band corresponding to CD79αβ under non-reducing conditions (1).
SDS-PAGE analysis of immunoprecipitates formed between the antibody and 125I-labeled, mildly denatured, and reduced lysate of Ramos B cells, shows weak reaction with a 43 kDa polypeptide corresponding to CD79α.
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: JCB117 (1). Isotype: IgG1, kappa.
Clone: JCB117 (1). Isotype: IgG1, kappa.
References
- Mason DY, Cordell JL, Brown MH, Borst J, Jones M, Pulford K, et al. CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood 1995;86:1453-9.
- 4. Nakamura T. BC20. CD79 workshop panel report. In: Kishimoto T, Kikutani H, von dem Borne AEG, Goyert SM, Mason DY, Miyasaka M, et al., editors. Leucocyte typing VI. White cell differentiation antigens. Proceedings of the 6th International Workshop and Conference; 1996 Nov 10-14; Kobe, Japan. New York, London: Garland Publishing Inc.; 1997. p. 180-2.
| Product | Size | | IR621 RTU, FLEX
| 60 tests, 12 mL Product No. IR62161 |
|---|
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human CD79α, Clone JCB117, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels B cells and is useful for the identification of B-cell neoplasms of all maturation stages (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human CD79α, Clone JCB117, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels B cells and is useful for the identification of B-cell neoplasms of all maturation stages (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Recombinant protein containing part of the extracellular portion of the human CD79α glycoprotein (1).
Specificity
Anti-Human CD79α, clone JCB117, was clustered as anti-CD79α at the Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens held in Kobe in 1996 (4).
In Western blotting of Ramos B-cell lysate, the antibody labels a band corresponding to CD79α under reducing conditions, and a band corresponding to CD79αβ under non-reducing conditions (1).
SDS-PAGE analysis of immunoprecipitates formed between the antibody and 125I-labeled, mildly denatured, and reduced lysate of Ramos B cells, shows weak reaction with a 43 kDa polypeptide corresponding to CD79α.
In Western blotting of Ramos B-cell lysate, the antibody labels a band corresponding to CD79α under reducing conditions, and a band corresponding to CD79αβ under non-reducing conditions (1).
SDS-PAGE analysis of immunoprecipitates formed between the antibody and 125I-labeled, mildly denatured, and reduced lysate of Ramos B cells, shows weak reaction with a 43 kDa polypeptide corresponding to CD79α.
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: JCB117 (1). Isotype: IgG1, kappa.
Clone: JCB117 (1). Isotype: IgG1, kappa.
References
- Mason DY, Cordell JL, Brown MH, Borst J, Jones M, Pulford K, et al. CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood 1995;86:1453-9.
- 4. Nakamura T. BC20. CD79 workshop panel report. In: Kishimoto T, Kikutani H, von dem Borne AEG, Goyert SM, Mason DY, Miyasaka M, et al., editors. Leucocyte typing VI. White cell differentiation antigens. Proceedings of the 6th International Workshop and Conference; 1996 Nov 10-14; Kobe, Japan. New York, London: Garland Publishing Inc.; 1997. p. 180-2.
| Product | Size | | IS621 RTU, FLEX
| 30 tests, 6 mL Product No. IS62130 |
|---|
Synonym(s) for Antigen
Ig-a, mb-1