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Monoclonal Mouse Anti-Human
Cytokeratin
Clone AE1/AE3
Reacts with the 65-67, 64, 59, 58, 56.5, 56, 54, 52, 50, 48 and 40 kDa cytokeratins.
The antibody labels keratinized and corneal epidermis, stratified squamous epithelia of internal organs, stratified epithelia, hyperproliferative keratinocytes, and simple epithelia. The antibody is useful for the identification of tumors as carcinoma or epithelial origin and for differential identification of undifferentiated carcinomas.
Isotype IgG1, kappa
- Frozen
- Formalin
- (Enzyme)/HIER
Seminoma (FFPE) stained with FLEX Anti-Cytokeratin, Code IR053/IS053.
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Seminoma. Focally, some neoplastic cells show a moderate cytoplasmic staining reaction, while the vast majorities are entirely nega-tive.
Clinical application for IR053/IS053. For identification of tumors of epithelial origin such as squamous cell carcinoma, seminoma and Merkel cell tumor.
Merkel cell tumor. Some neoplastic cells show a focal and dot-like cytoplasmic staining reaction for cytokeratin.
Clinical application for IR053/IS053. For identification of tumors of epithelial origin such as squamous cell carcinoma, seminoma and Merkel cell tumor.
Tonsil. The squamous mucosa show a strong and diffuse cytoplasmic staining reaction in all cell layers. The interdigitating reticulum cells of the lymphoid tissue can be positive.
Recommended control for IR053/IS053. Tonsil/Liver.
Liver. The majority of the liver cells show a moderate predominantly membranous staining reaction, and the bile duct epithelial cells show a strong cytoplasmic staining reaction.
Recommended control for IR053/IS053. Tonsil/Liver.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human Cytokeratin, Clone AE1/AE3 is intended for laboratory use to identify qualitatively by light microscopy two epitopes present on a majority of epithelial cytokeratins in acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue using immunohistochemical (IHC) test methods. Positive results aid in the classification of normal and neoplastic tissue as epithelial in origin (1-3) and serve as an adjunct to conventional histopathology. The clinical interpretation of any positive staining or its absence should be complemented by morphological and histological studies with proper controls. Evaluations should be made within the context of the patient's clinical history and other diagnostic tests by a qualified individual.
Monoclonal Mouse Anti-Human Cytokeratin, Clone AE1/AE3 is intended for laboratory use to identify qualitatively by light microscopy two epitopes present on a majority of epithelial cytokeratins in acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue using immunohistochemical (IHC) test methods. Positive results aid in the classification of normal and neoplastic tissue as epithelial in origin (1-3) and serve as an adjunct to conventional histopathology. The clinical interpretation of any positive staining or its absence should be complemented by morphological and histological studies with proper controls. Evaluations should be made within the context of the patient's clinical history and other diagnostic tests by a qualified individual.
Immunogen
Human epidermal callus (1).
Specificity
AE1/AE3 is a cocktail of two monoclonal antibodies that were obtained by immunizing mice with human callus keratins (2). AE1/AE3 has been shown to identify the majority of human cytokeratins and thus may be used as a tool for the positive IHC identification of cells of simple and stratified epithelial origin (1,2,4). Antibody AE1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with Moll's designation (4) 10, 13, 14, 15 16 and 19 (MWs of 56.5, 54', 50, 50', 48 and 40 kDa, respectively) but not on Nos. 12, 17 and 18 (55, 47 and 45 kDa) (4). Antibody AE3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including Nos. 1 and 2, 3, 4, 5, 6, 7 and 8 (MWs of 65, 67, 64, 59, 58, 56, 54 and 52 kDa, respectively) (5).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as tissue culture supernatant in 0.05 mol/L Tris-HCl, pH 7.2 and 0.015 mol/L sodium azide. This product contains stabilizing protein.
Clone: AE1/AE3 (1, 2). Isotype: IgG1, kappa
Mouse lgG concentration mg/L: See label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
Clone: AE1/AE3 (1, 2). Isotype: IgG1, kappa
Mouse lgG concentration mg/L: See label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
References
- Tseng SCG, Jarvinen MJ, Nelson WG, Huang JW, Woodcock-Mitchell J, Sun TTl. Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies. Cell 1982;30:361-72.
- Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT. Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol 1982;95(2 Pt 1):580-8.
- Moll R, Franke WW, Schiller Dl. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982;31:11-24.
- Sun T-T, Eichner R, Schermer A, Cooper D, Nelson WG, Weiss RA. Classification, expression and possible mechanisms of evolution of mammalian epithelial keratins: A unifying model. In: Levine A, Topp W, Vande Woude G, Watson JD (eds.). Cancer cells 1 the transformed phenotype. New York: Cold Spring Harbor Laboratory 1984
- Eichner R, Bonitz P, Sun T-T. Classification of epidermal keratins according to their immunoreactivity, isoelectric point and mode of expression. J Cell Biol 1984;98:1388-96.
| Product | Size |  | M3515 Ascites
| 0.2 mL Product No. M351529 |
|---|---|---|
| 1 mL Product No. M351501 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Cytokeratin, Clone AE1/AE3, Ready-to-Use, (Link), is intended for use in immunohistochemistry with Autostainer Link instruments. This antibody is useful for the identification of tumors as carcinoma or epithelial origin (1-3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Cytokeratin, Clone AE1/AE3, Ready-to-Use, (Link), is intended for use in immunohistochemistry with Autostainer Link instruments. This antibody is useful for the identification of tumors as carcinoma or epithelial origin (1-3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Human epidermal callus (1).
Specificity
AE1/AE3 is a cocktail of two monoclonal antibodies that were obtained by immunizing mice with human callus keratins (2). AE1/AE3 has been shown to identify the majority of human cytokeratins and thus may be used as a tool for the positive IHC identification of cells of simple and stratified epithelial origin (1,2,4). Antibody AE1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with Moll's designation (4) 10, 13, 14, 15 16 and 19 (MWs of 56.5, 54', 50, 50', 48 and 40 kDa, respectively) but not on Nos. 12, 17 and 18 (55, 47 and 45 kDa) (4). Antibody AE3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including Nos. 1 and 2, 3, 4, 5, 6, 7 and 8 (MWs of 65, 67, 64, 59, 58, 56, 54 and 52 kDa, respectively) (5).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
Clone: AE1/AE3. Isotype: IgG1, kappa
Clone: AE1/AE3. Isotype: IgG1, kappa
References
- Tseng SCG, Jarvinen MJ, Nelson WG, Huang JW, Woodcock-Mitchell J, Sun TTl. Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies. Cell 1982; 30:361
- Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT. Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol 1982; 95:580-8.
- Moll R, Franke WW, Schiller Dl. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982; 31:11-24.
- Sun T-T, Eichner R, Schermer A, Cooper D, Nelson WG, Weiss RA. Classification, expression and possible mechanisms of evolution of mammalian epithelial keratins: A unifying model. In: Levine A, Topp W, Vande Woude G, Watson JD (eds.). Cancer cells 1 the transformed phenotype. New York: Cold Spring Harbor Laboratory 1984
- Eichner R, Bonitz P, Sun T-T. Classification of epidermal keratins according to their immunoreactivity, isoelectric point and mode of expression. J Cell Biol 1984; 98:1388-96.
| Product | Size | | IR053 RTU, FLEX
| 60 tests, 12 mL Product No. IR05361 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Squamous mucosa | 120 tests, 24 mL Product No. IR75066 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | Silanized Slides (Code S3003) Recommended for greater adherence Alternative is FLEX IHC Microscope Slides (Code K8020) | 100 slides Product No. S300330 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Cytokeratin, Clone AE1/AE3, Ready-to-Use, (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of tumors as carcinoma or epithelial origin (1-3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Cytokeratin, Clone AE1/AE3, Ready-to-Use, (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of tumors as carcinoma or epithelial origin (1-3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Human epidermal callus (1).
Specificity
AE1/AE3 is a cocktail of two monoclonal antibodies that were obtained by immunizing mice with human callus keratins (2). AE1/AE3 has been shown to identify the majority of human cytokeratins and thus may be used as a tool for the positive IHC identification of cells of simple and stratified epithelial origin (1,2,4). Antibody AE1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with Moll's designation (4) 10, 13, 14, 15 16 and 19 (MWs of 56.5, 54', 50, 50', 48 and 40 kDa, respectively) but not on Nos. 12, 17 and 18 (55, 47 and 45 kDa) (4). Antibody AE3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including Nos. 1 and 2, 3, 4, 5, 6, 7 and 8 (MWs of 65, 67, 64, 59, 58, 56, 54 and 52 kDa, respectively) (5).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
Clone: AE1/AE3. Isotype: IgG1, kappa
Clone: AE1/AE3. Isotype: IgG1, kappa
References
- Tseng SCG, Jarvinen MJ, Nelson WG, Huang JW, Woodcock-Mitchell J, Sun TTl. Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies. Cell 1982; 30:361
- Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT. Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol 1982; 95:580
- Moll R, Franke WW, Schiller Dl. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982; 31:11
- Sun T-T, Eichner R, Schermer A, Cooper D, Nelson WG, Weiss RA. Classification, expression and possible mechanisms of evolution of mammalian epithelial keratins: A unifying model. In: Levine A, Topp W, Vande Woude G, Watson JD (eds.). Cancer cells 1 the transformed phenotype. New York: Cold Spring Harbor Laboratory 1984
- Eichner R, Bonitz P, Sun T-T. Classification of epidermal keratins according to their immunoreactivity, isoelectric point and mode of expression. J Cell Biol 1984; 98:1388
| Product | Size | | IS053 RTU, FLEX
| 30 tests, 6 mL Product No. IS05330 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Squamous mucosa | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | Silanized Slides (Code S3003) Recommended for greater adherence Alternative is FLEX IHC Microscope Slides (Code K8020) | 100 slides Product No. S300330 |
Synonym(s) for Antigen
Keratin, CK, ae1ae3, pankeratin