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Monoclonal Mouse Anti-Human
Cytokeratin 10/13
Clone DE-K13
Reacts on formalin-fixed, paraffin-embedded tissue sections with the 53 kDa protein corresponding to cytokeratin 13. This cytokeratin is present in several non-cornified, stratified squamous epithelia, for example tongue mucosa, and tracheal and anal canal epithelium. On frozen sections the antibody, in addition, reacts with the 56.5 kDa protein corresponding to cytokeratin 10.
Isotype IgG2a, kappa
- Frozen
- Formalin
- HIER
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human Cytokeratin 10/13, Clone DE-K13, is recommended for use in immunohistochemistry. In formalin-fixed, paraffin-embedded tissue the antibody labels suprabasal cell layers of non-cornifying stratified epithelia, corresponding to cytokeratin 13 (1, 2), while in frozen sections, the antibody labels suprabasal cell layers of both cornifying and non-cornifying stratified epithelia, corresponding to cytokeratin 10 and 13 (3). The clinical interpretation of any positive staining or its absence should be complemented by morphological and histological studies with proper controls. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human Cytokeratin 10/13, Clone DE-K13, is recommended for use in immunohistochemistry. In formalin-fixed, paraffin-embedded tissue the antibody labels suprabasal cell layers of non-cornifying stratified epithelia, corresponding to cytokeratin 13 (1, 2), while in frozen sections, the antibody labels suprabasal cell layers of both cornifying and non-cornifying stratified epithelia, corresponding to cytokeratin 10 and 13 (3). The clinical interpretation of any positive staining or its absence should be complemented by morphological and histological studies with proper controls. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Cytoskeletal preparation extracted from human ectocervical epithelium (3).
Specificity
In two-dimensional immunoblotting of A431 cells, a human vulvar squamous carcinoma cell line, the antibody labels dots corresponding to cytokeratin 13. The antibody also labels cytokeratin 10 in immunoblotting of cytokeratin-enriched cytoskeletal proteins isolated from human tissue (3).
The antibody cross-reacts with CK 10/13 of feline origin (3).
The antibody cross-reacts with CK 10/13 of feline origin (3).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: DE-K13 (3). Isotype: IgG2a, kappa.
Mouse IgM concentration: See label on vial.
Clone: DE-K13 (3). Isotype: IgG2a, kappa.
Mouse IgM concentration: See label on vial.
References
- Itakura Y, Sasano H, Abe K, Furukawa Y, Mori S, Nagura H. Cytokeratin immunolocalization and lectin binding studies in oesophageal squamous dysplasis. Histopathology 1996;29:3-10.
- van Bommel PFJ, Kenemans P, Helmerhorst TJM, Gallee MPW, Ivanyi D. Expression of cytokeratin 10,13, and involucrin as prognostic factors in low stage squamous cell carcinoma of the uterine cervix. Cancer 1994;74:2314-20.
- Ivanyi D, Minke JMHM, Hageman C, Groeneveld E, van Doornewaard G. Patterns of expressionof feline cytokeratins in healthy epithelia and mammary carcinoma cells. Am J Vet Res 1992;53:304-14.
| Product | Size |  | M7003 Culture supernatant
| 1 mL Product No. M700301 |
|---|