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Monoclonal Mouse Anti-Human
Cytokeratin 18
Clone DC 10
Reacts with the 45 kDa protein corresponding to cytokeratin 18. The antibody labels a large number of simple epithelia, including many ductal and glandular epithelia.
The antibody labels tumor cells of epithelial origin, whereas tumor cells of non-epithelial origin such as glioma, melanoma and osteosarcoma, are not labeled. In vascular tumors, the antibody labels endothelial cells in epithelioid hemangioendotheliomas.
Isotype IgG1, kappa
- Frozen
- Formalin
- Enzyme/HIER
References
- Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 1988;7:495-504.
Liver stained with FLEX Anti-Cytokeratin 18, Code IR618/IS618.
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Liver. The bile duct epithelial cells show a moderate to strong cytoplasmic staining reaction, whereas the liver cells show a weak to moderate staining reaction.The staining reaction can be heterogeneous with the strongest staining in the periportal zones. Kupffer cells should be negative.
Recommended control for IR618/IS618. Liver.
Merkel cell carcinoma. The majority of the neoplastic cells show a distinct cytoplasmic and focally dot-like staining reaction.
Clinical application for IR618/IS618. For identification of some epithelial tumors like Merkel cell carcinoma, seminoma and epithelioid hemangioendothelioma.
Seminoma. Several neoplastic cells show a focal and distinct cytoplasmic staining reaction - in some areas the staining may be more dot-like. The majority of the neoplastic cells are negative.
Clinical application for IR618/IS618. For identification of some epithelial tumors like Merkel cell carcinoma, seminoma and epithelioid hemangioendothelioma.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human Cytokeratin 18, Clone DC 10, is intended for use in immunohistochemistry. The antibody labels epithelial cells in those epithelia expressing cytokeratin 18, and hence the antibody is a useful tool for the differentiation and identification of epithelial tumors (1) and epithelioid hemangioendotheliomas (2). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient´s clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human Cytokeratin 18, Clone DC 10, is intended for use in immunohistochemistry. The antibody labels epithelial cells in those epithelia expressing cytokeratin 18, and hence the antibody is a useful tool for the differentiation and identification of epithelial tumors (1) and epithelioid hemangioendotheliomas (2). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient´s clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
PCM 42, human breast carcinoma cell line (6).
Specificity
In Western blotting of cytokeratin preparations from BT 20 cells, the antibody labels a single band of 45 kDa corresponding to CK 18 (6).
In immunocytochemistry on human cultured cell lines, the antibody labels tumor cells of epithelial origin, whereas normal fibroblasts and tumor cells of non-epithelial origin such as glioma, melanoma and osteosarcoma, are not labeled (6).
As demonstrated by immunocytochemistry, the antibody cross-reacts with the CK 18-equivalent protein in monkey (7). No cross-reactivity with the CK 18-equivalent protein is observed in cow, dog, hamster, mouse, rat, sheep and swine (6).
In immunocytochemistry on human cultured cell lines, the antibody labels tumor cells of epithelial origin, whereas normal fibroblasts and tumor cells of non-epithelial origin such as glioma, melanoma and osteosarcoma, are not labeled (6).
As demonstrated by immunocytochemistry, the antibody cross-reacts with the CK 18-equivalent protein in monkey (7). No cross-reactivity with the CK 18-equivalent protein is observed in cow, dog, hamster, mouse, rat, sheep and swine (6).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: DC 10 (6). Isotype: IgG1, kappa.
Mouse IgG concentration: See label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
Clone: DC 10 (6). Isotype: IgG1, kappa.
Mouse IgG concentration: See label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
References
- Lane EB, Alexander CM. Use of keratin antibodies in tumor diagnosis [review]. Semin Cancer Biol 1990;1:165-79.
- Miettinen M, Fetsch JF. Distribution of keratins in normal endothelial cells and a spectrum of vascular tumors: Implications in tumor diagnosis. Hum Pathol 2000;31:1062-7.
- Lauerová L, Kovarik J, Bártek J, Rejthar A, Vojteek B. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 1988;7:495-504.
- Sculean A, Berakdar M, Pahl S, Windisch P, Brecx M, Reich E, et al. Patterns of cytokeratin expression in monkey and human periodontium following regenerative and conventional periodontal surgery. J Periodont Res 2001;36:260-8.
| Product | Size |  | M7010 Culture supernatant
| 0.2 mL Product No. M701029 |
|---|
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Cytokeratin 18, Clone DC 10, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels epithelial cells in those epithelia expressing cytokeratin 18, and hence the antibody is a useful tool for the differentiation and identification of epithelial tumors (1) and epithelioid hemangioendotheliomas (2). Antibodies to cytokeratin 18 may also be useful for the identification of seminomas (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Cytokeratin 18, Clone DC 10, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels epithelial cells in those epithelia expressing cytokeratin 18, and hence the antibody is a useful tool for the differentiation and identification of epithelial tumors (1) and epithelioid hemangioendotheliomas (2). Antibodies to cytokeratin 18 may also be useful for the identification of seminomas (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
PCM 42, human breast carcinoma cell line (7).
Specificity
In Western blotting of cytokeratin preparations from BT 20 cells, the antibody labels a single band of 45 kDa corresponding to CK18 (7).
In immunocytochemistry on human cultured cell lines, the antibody labels tumor cells of epithelial origin, whereas normal fibroblasts and tumor cells of non-epithelial origin such as glioma, melanoma and osteosarcoma, are not labeled (7).
In immunocytochemistry on human cultured cell lines, the antibody labels tumor cells of epithelial origin, whereas normal fibroblasts and tumor cells of non-epithelial origin such as glioma, melanoma and osteosarcoma, are not labeled (7).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: DC 10 (7). Isotype: IgG1, kappa.
Clone: DC 10 (7). Isotype: IgG1, kappa.
References
- Lane EB, Alexander CM. Use of keratin antibodies in tumor diagnosis [review]. Semin Cancer Biol 1990; 1:165-79.
- Miettinen M, Fetsch JF. Distribution of keratins in normal endothelial cells and a spectrum of vascular tumors: Implications in tumor diagnosis. Hum Pathol 2000; 31:1062-7.
- Fogel M, Lifschitz-Mercer B, Moll R, Kushnir I, Jacob N, Waldherr R, et al. Heterogeneity of intermediate filament expression in human testicular seminomas. Differentiation 1990;45:242-9.
- Lauerová L, Kovarik J, Bártek J, Rejthar A, Vojteek B. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 1988; 7:495-504.
| Product | Size | | IR618 RTU, FLEX
| 60 tests, 12 mL Product No. IR61861 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Liver | 120 tests, 24 mL Product No. IR75066 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Cytokeratin 18, Clone DC 10, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels epithelial cells in those epithelia expressing cytokeratin 18, and hence the antibody is a useful tool for the differentiation and identification of epithelial tumors (1) and epithelioid hemangioendotheliomas (2). Antibodies to cytokeratin 18 may also be useful for the identification of seminomas (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Cytokeratin 18, Clone DC 10, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels epithelial cells in those epithelia expressing cytokeratin 18, and hence the antibody is a useful tool for the differentiation and identification of epithelial tumors (1) and epithelioid hemangioendotheliomas (2). Antibodies to cytokeratin 18 may also be useful for the identification of seminomas (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
PCM 42, human breast carcinoma cell line (7).
Specificity
In Western blotting of cytokeratin preparations from BT 20 cells, the antibody labels a single band of 45 kDa corresponding to CK18 (7).
In immunocytochemistry on human cultured cell lines, the antibody labels tumor cells of epithelial origin, whereas normal fibroblasts and tumor cells of non-epithelial origin such as glioma, melanoma and osteosarcoma, are not labeled (7).
In immunocytochemistry on human cultured cell lines, the antibody labels tumor cells of epithelial origin, whereas normal fibroblasts and tumor cells of non-epithelial origin such as glioma, melanoma and osteosarcoma, are not labeled (7).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: DC 10 (7). Isotype: IgG1, kappa.
Clone: DC 10 (7). Isotype: IgG1, kappa.
References
- Lane EB, Alexander CM. Use of keratin antibodies in tumor diagnosis [review]. Semin Cancer Biol 1990; 1:165-79.
- Miettinen M, Fetsch JF. Distribution of keratins in normal endothelial cells and a spectrum of vascular tumors: Implications in tumor diagnosis. Hum Pathol 2000; 31:1062-7.
- Fogel M, Lifschitz-Mercer B, Moll R, Kushnir I, Jacob N, Waldherr R, et al. Heterogeneity of intermediate filament expression in human testicular seminomas. Differentiation 1990;45:242-9.
- Lauerová L, Kovarik J, Bártek J, Rejthar A, Vojteek B. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 1988; 7:495-504.
| Product | Size | | IS618 RTU, FLEX
| 30 tests, 6 mL Product No. IS61830 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Liver | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
Synonym(s) for Antigen
CK18, keratin