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Monoclonal Mouse Anti-Human
Epithelial Antigen
Clone Ber-EP4
Epithelial antigen is a transmembrane glycoprotein functioning as a cellular adhesion molecule.
This epithelium-specific antigen is broadly distributed in epithelial cells, and displays a highly conserved expression in carcinomas. The antibody is useful in the differential diagnosis of adenocarcinoma versus malignant mesothelioma. Anti-Epithelial Antigen may also aid in detection of micrometastases in lymph nodes of patients with esophageal carcinoma as well as in differentiation between basal and squamous cell carcinomas of the skin.
Isotype IgG1, kappa
- Frozen
- Formalin
- HIER
Lung adenocarcinoma (FFPE) stained with FLEX Anti-Epithelial Antigen, Code IR637/IS637.
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Lung adenocarcinoma. The majority of the neoplastic cells show a distinct predominantly membranous staining reaction.
Clinical application for IR637/IS637 For differential diagnosis of adenocarcinoma vs. malignant mesothelioma and basal cell carcinoma vs. squamous cell carcinoma of the skin.
Mesothelioma. The majority of the neoplastic cells are negative. Only a few cells show a positive staining reaction.
Clinical application for IR637/IS637 For differential diagnosis of adenocarcinoma vs. malignant mesothelioma and basal cell carcinoma vs. squamous cell carcinoma of the skin.
Colon. The columnar epithelial cells should show a moderate to strong staining reaction. No staining reaction should be seen in lymphocytes apart from a cytoplasmic staining reaction in macrophages in lamina propria.
Recommended control for IR637/IS637. Appendix/Colon/Kidney.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human Epithelial Antigen, Clone Ber-EP4, is intended for use in immunohistochemistry. The antibody labels most epithelial cells and is useful as a discriminant in the differential diagnosis of adenocarcinoma versus malignant mesothelioma (1). The antibody may also aid in the detection of micrometastases in lymph nodes of patients with oesophageal carcinoma (2) and in differentiating between basal and squamous cell carcinomas of the skin (3). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human Epithelial Antigen, Clone Ber-EP4, is intended for use in immunohistochemistry. The antibody labels most epithelial cells and is useful as a discriminant in the differential diagnosis of adenocarcinoma versus malignant mesothelioma (1). The antibody may also aid in the detection of micrometastases in lymph nodes of patients with oesophageal carcinoma (2) and in differentiating between basal and squamous cell carcinomas of the skin (3). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
MCF-7 cells (human breast carcinoma cell line) (4).
Specificity
Analysis of immunocomplexes between the antibody and lysate of 125I surface-labeled MCF-7 cells in SDS-PAGE under reducing conditions shows that the antibody labels two polypeptides of 34 kDa and 39 kDa, respectively, corresponding to epithelial antigen. Under non-reducing conditions, the polypeptides appear as 39 kDa and 41 kDa, while deglycosylation reduces the size to 31 kDa, and 36 kDa.
In immunoprecipitation experiments, the Ber-EP4 antibody blocks the reaction of the HEA125 antibody with MCF-7 cell lysate and vice versa, showing that the two antibodies react with the same antigen. The two antibodies also produce identical staining results in cells and tissues (4).
Of 37 cell lines tested, the antibody homogeneously labels all (10/10) carcinoma cell lines, whereas all non-epithelial cell lines (26/27) are negative except for the erythromyeloid cell line K562 (4).
In immunoprecipitation experiments, the Ber-EP4 antibody blocks the reaction of the HEA125 antibody with MCF-7 cell lysate and vice versa, showing that the two antibodies react with the same antigen. The two antibodies also produce identical staining results in cells and tissues (4).
Of 37 cell lines tested, the antibody homogeneously labels all (10/10) carcinoma cell lines, whereas all non-epithelial cell lines (26/27) are negative except for the erythromyeloid cell line K562 (4).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: Ber-EP4 (4). Isotype: IgG1, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
Clone: Ber-EP4 (4). Isotype: IgG1, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
References
- Sheibani K, Shin SS, Kezirian J, Weiss LM. Ber-EP4 antibody as a discriminant in the differential diagnosis of malignant mesothelioma versus adenocarcinoma. Am J Surg Pathol 1991;15:779-84.
- Hosch S, Kraus J, Scheunemann P, Izbicki JR, Schneider C, Schumacher U, et al. Malignant potential and cytogenetic characteristics of occult disseminated tumor cells in esophageal cancer. Cancer Res 2000;60:6836-40.
- Beer TW, Shepherd P, Theaker JM. Ber EP4 and epithelial membrane antigen aid distinction of basal cell, squamous cell and basosquamous carcinomas of the skin. Histopathology 2000;37:218-23.
- Latza U, Niedobitek G, Schwarting R, Nekarda H, Stein H. Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelia. J Clin Pathol 1990;43:213-9.
| Product | Size |  | M0804 Culture supernatant
| 0.2 mL Product No. M080429 |
|---|---|---|
| 1 mL Product No. M080401 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Epithelial Antigen, Clone Ber-EP4, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels most epithelial cells and is useful in the differential diagnosis of adenocarcinoma versus malignant mesothelioma (1). The antibody may also aid in the detection of micrometastases in lymph nodes of patients with esophageal carcinoma (2) and in differentiating between basal and squamous cell carcinomas of the skin (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Epithelial Antigen, Clone Ber-EP4, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels most epithelial cells and is useful in the differential diagnosis of adenocarcinoma versus malignant mesothelioma (1). The antibody may also aid in the detection of micrometastases in lymph nodes of patients with esophageal carcinoma (2) and in differentiating between basal and squamous cell carcinomas of the skin (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
MCF-7 cells (human breast carcinoma cell line) (4).
Specificity
Analysis of immunocomplexes between the antibody and lysate of 125I surface-labeled MCF-7 cells in SDS-PAGE under reducing conditions shows that the antibody labels two polypeptides of 34 kDa and 39 kDa, respectively, corresponding to epithelial antigen. Under non-reducing conditions, the polypeptides appear as 39 kDa and 41 kDa, while deglycosylation reduces the size to 31 kDa, and 36 kDa.
In immunoprecipitation experiments, the Ber-EP4 antibody blocks the reaction of the HEA125 antibody with MCF-7 cell lysate and vice versa, showing that the two antibodies react with the same antigen. The two antibodies also produce identical staining results in cells and tissues (4).
Of 37 cell lines tested, the antibody homogeneously labels all (10/10) carcinoma cell lines, whereas all non-epithelial cell lines (26/27) are negative except for the erythromyeloid cell line K562 (4).
In immunoprecipitation experiments, the Ber-EP4 antibody blocks the reaction of the HEA125 antibody with MCF-7 cell lysate and vice versa, showing that the two antibodies react with the same antigen. The two antibodies also produce identical staining results in cells and tissues (4).
Of 37 cell lines tested, the antibody homogeneously labels all (10/10) carcinoma cell lines, whereas all non-epithelial cell lines (26/27) are negative except for the erythromyeloid cell line K562 (4).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: Ber-EP4 (4). Isotype: IgG1, kappa.
Clone: Ber-EP4 (4). Isotype: IgG1, kappa.
References
- Sheibani K, Shin SS, Kezirian J, Weiss LM. Ber-EP4 antibody as a discriminant in the differential diagnosis of malignant mesothelioma versus adenocarcinoma. Am J Surg Pathol 1991;15:779-84.
- Hosch S, Kraus J, Scheunemann P, Izbicki JR, Schneider C, Schumacher U, et al. Malignant potential and cytogenetic characteristics of occult disseminated tumor cells in esophageal cancer. Cancer Res 2000;60:6836-40.
- Beer TW, Shepherd P, Theaker JM. Ber EP4 and epithelial membrane antigen aid distinction of basal cell, squamous cell and basosquamous carcinomas of the skin. Histopathology 2000;37:218-23.
- Latza U, Niedobitek G, Schwarting R, Nekarda H, Stein H. Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelia. J Clin Pathol 1990;43:213-9.
| Product | Size | | IR637 RTU, FLEX
| 60 tests, 12 mL Product No. IR63761 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, Low pH (Code K8005) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800521 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Colon and kidney | 120 tests, 24 mL Product No. IR75066 |
| Visualization System | EnVision™ FLEX (Code K8000) Use Low pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Epithelial Antigen, Clone Ber-EP4, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels most epithelial cells and is useful in the differential diagnosis of adenocarcinoma versus malignant mesothelioma (1). The antibody may also aid in the detection of micrometastases in lymph nodes of patients with esophageal carcinoma (2) and in differentiating between basal and squamous cell carcinomas of the skin (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Epithelial Antigen, Clone Ber-EP4, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels most epithelial cells and is useful in the differential diagnosis of adenocarcinoma versus malignant mesothelioma (1). The antibody may also aid in the detection of micrometastases in lymph nodes of patients with esophageal carcinoma (2) and in differentiating between basal and squamous cell carcinomas of the skin (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
MCF-7 cells (human breast carcinoma cell line) (4).
Specificity
Analysis of immunocomplexes between the antibody and lysate of 125I surface-labeled MCF-7 cells in SDS-PAGE under reducing conditions shows that the antibody labels two polypeptides of 34 kDa and 39 kDa, respectively, corresponding to epithelial antigen. Under non-reducing conditions, the polypeptides appear as 39 kDa and 41 kDa, while deglycosylation reduces the size to 31 kDa, and 36 kDa.
In immunoprecipitation experiments, the Ber-EP4 antibody blocks the reaction of the HEA125 antibody with MCF-7 cell lysate and vice versa, showing that the two antibodies react with the same antigen. The two antibodies also produce identical staining results in cells and tissues (4).
Of 37 cell lines tested, the antibody homogeneously labels all (10/10) carcinoma cell lines, whereas all non-epithelial cell lines (26/27) are negative except for the erythromyeloid cell line K562 (4).
In immunoprecipitation experiments, the Ber-EP4 antibody blocks the reaction of the HEA125 antibody with MCF-7 cell lysate and vice versa, showing that the two antibodies react with the same antigen. The two antibodies also produce identical staining results in cells and tissues (4).
Of 37 cell lines tested, the antibody homogeneously labels all (10/10) carcinoma cell lines, whereas all non-epithelial cell lines (26/27) are negative except for the erythromyeloid cell line K562 (4).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: Ber-EP4 (4). Isotype: IgG1, kappa.
Clone: Ber-EP4 (4). Isotype: IgG1, kappa.
References
- Sheibani K, Shin SS, Kezirian J, Weiss LM. Ber-EP4 antibody as a discriminant in the differential diagnosis of malignant mesothelioma versus adenocarcinoma. Am J Surg Pathol 1991;15:779-84.
- Hosch S, Kraus J, Scheunemann P, Izbicki JR, Schneider C, Schumacher U, et al. Malignant potential and cytogenetic characteristics of occult disseminated tumor cells in esophageal cancer. Cancer Res 2000;60:6836-40.
- Beer TW, Shepherd P, Theaker JM. Ber EP4 and epithelial membrane antigen aid distinction of basal cell, squamous cell and basosquamous carcinomas of the skin. Histopathology 2000;37:218-23.
- Latza U, Niedobitek G, Schwarting R, Nekarda H, Stein H. Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelia. J Clin Pathol 1990;43:213-9.
| Product | Size | | IS637 RTU, FLEX
| 30 tests, 6 mL Product No. IS63730 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, Low pH (Code K8005) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800521 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Colon and kidney | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use Low pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
Synonym(s) for Antigen
BerEP4