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Monoclonal Mouse Anti-Human
CD19
Clone LE-CD19
CD19 is the broadest lineage-specific surface marker for B cells.
CD19 is present on the surface of virtually all B lymphocytes, including early B-progenitor cells, but it is lost upon terminal differentiation to plasma cells. CD19 is also expressed on follicular dendritic cells (1). B-lineage leukemias and lymphomas rarely lose the CD19 antigen (2).
Isotype IgG1, kappa
- Frozen
- Formalin
- HIER
References
- Sato S, Tedder TF. BC3. CD19 workshop panel report. In: Kishimoto T, Kikutani H, von dem Borne AEG, Goyert SM, Mason DY, Miyasaka M, et al., editors. Leucocyte typing VI. White cell differentiation antigens. Proceedings of the 6th International Workshop and Conference; 1996 Nov 10-14; Kobe, Japan. New York, London: Garland Publishing Inc.; 1997. p. 133-5.
- Scheuermann RH, Racila E. CD19 antigen in leukaemia and lymphoma diagnosis and immunotherapy (review). Leuk Lymphoma 1995;18:385-97.
Precursor B-cell lymphoblastic leukemia/lymphoma (FFPE) stained with FLEX Anti-CD19, Code IR656/IS656.
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Precursor B-cell lymphoblastic leukemia/lymphoma. The majority of the neoplastic cells show a moderate to strong predominantly membranous staining reaction.
Clinical application for IR656/IS656. For identification of B cells in normal and neoplastic tissue.
B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma. The majority of the neoplastic cells show a moderate to strong predominantly membranous staining reaction.
Clinical application for IR656/IS656. For identification of B cells in normal and neoplastic tissue.
Tonsil. The B cells in the mantle zone and in the germinal center show a moderate to strong membranous staining reaction. No staining should be seen in the epithelial cells.
Recommended control for IR656/IS656. Appendix/Colon/Tonsil.
Colon. The plasma cells in the lamina propria show a weak to moderate membranous staining reaction.
Recommended control for IR656/IS656. Appendix/Colon/Tonsil.
Lymphoma stained with Anti-CD19.
Clinical application for IR656/IS656. For identification of B cells in normal and neoplastic tissue.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human CD19, Clone LE-CD19, is intended for use in immunohistochemistry. This antibody labels B cells in normal and neoplastic tissue. Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human CD19, Clone LE-CD19, is intended for use in immunohistochemistry. This antibody labels B cells in normal and neoplastic tissue. Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Synthetic peptide comprising the C-terminus of CD19.
Specificity
In Western blotting of lysed Raji cells (Burkitt's lymphoma) and Jurkat cells (T-ALL) under reduced conditions, the antibody labels a band of 95 kDa in Raji cells, corresponding to CD19, whereas no band is observed in Jurkat cells.
As demonstrated by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections, the antibody does not cross-react with the CD19-equivalent protein in cow, dog, horse, mouse, rat or swine.
As demonstrated by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections, the antibody does not cross-react with the CD19-equivalent protein in cow, dog, horse, mouse, rat or swine.
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant (containing fetal bovine serum) dialyzed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 0.015 mol/L sodium azide.
Clone: LE-CD19. Isotype: IgG1, kappa.
Mouse IgG concentration mg/L: See label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
Clone: LE-CD19. Isotype: IgG1, kappa.
Mouse IgG concentration mg/L: See label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
| Product | Size |  | M7296 Culture supernatant
| 0.2 mL Product No. M729629 |
|---|
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human CD19, Clone LE-CD19, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels B cells in normal and neoplastic tissue. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human CD19, Clone LE-CD19, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels B cells in normal and neoplastic tissue. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Synthetic peptide comprising the C-terminus of CD19.
Specificity
In Western blotting of lysed Raji cells (Burkitt's lymphoma) and Jurkat cells (T-ALL) under reduced conditions, the antibody labels a band of 95 kDa in Raji cells, corresponding to CD19, whereas no band is observed in Jurkat cells.
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: LE-CD19. Isotype: IgG1, kappa.
Clone: LE-CD19. Isotype: IgG1, kappa.
| Product | Size | | IR656 RTU, FLEX
| 60 tests, 12 mL Product No. IR65661 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Tonsil and colon | 120 tests, 24 mL Product No. IR75066 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human CD19, Clone LE-CD19, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels B cells in normal and neoplastic tissue. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human CD19, Clone LE-CD19, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels B cells in normal and neoplastic tissue. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Synthetic peptide comprising the C-terminus of CD19.
Specificity
In Western blotting of lysed Raji cells (Burkitt's lymphoma) and Jurkat cells (T-ALL) under reduced conditions, the antibody labels a band of 95 kDa in Raji cells, corresponding to CD19, whereas no band is observed in Jurkat cells.
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: LE-CD19. Isotype: IgG1, kappa.
Clone: LE-CD19. Isotype: IgG1, kappa.
| Product | Size | | IS656 RTU, FLEX
| 30 tests, 6 mL Product No. IS65630 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Tonsil and colon | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |