TOP2A IQFISH pharmDx is a direct fluorescence in situ hybridization (FISH) assay based on Dako's new fast IQISH hybridization buffer chemistry. The IQISH hybridization buffer is non-toxic and allows genomic DNA probe hybridization to be performed in just 60-120 minutes. The short hybridization time results in a turnaround time of about 3½ hours for a complete FISH staining from deparaffinization to mounting.

TOP2A IQFISH pharmDx is designed to detect amplifications and deletions (copy number changes) of the TOP2A gene using fluorescence in situ hybridization (FISH) technique on formalin-fixed, paraffin-embedded human breast cancer tissue specimens. Deletions and amplifications of the TOP2A gene serve as markers for poor prognosis in high-risk breast cancer patients. TOP2A gene amplification detected by the TOP2A IQFISH pharmDx is further indicated as an aid to predict recurrence-free and overall survival in high-risk breast cancer patients treated with adjuvant epirubicin-based chemotherapy.

Results from the TOP2A IQFISH pharmDx are intended for use as an adjunct to existing clinical and pathological information. Topoisomerase IIα is a key enzyme involved in DNA replication and is the molecular target for topoisomerase II inhibitor therapies. Clinical research shows that the TOP2A gene can be used as a predictive indicator of susceptibility or resistance to anthracycline therapies (1-3).

Breast carcinoma (FFPE) stained with TOP2A IQFISH pharmDx, Code K5733. Tumor cells show TOP2A gene amplification (TOP2A/CEN-17 ratio ≥ 2).

Breast carcinoma (FFPE) stained with TOP2A IQFISH pharmDx, Code K5733. Tumor cells show TOP2A gene deletion (TOP2A/CEN-17 ratio < 2).

Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.

Country selection

Your Dako Account indicates that you are based in , you will be transfered to the web site for

Basket emptied

Your basket was emptied because of the country change.

Country selection

United States of America appears to be your location. To view country specific information on the Dako web site, please accept or change country below.

Dako Cookie Policy
This site uses cookies. By continuing to browse the site, you are agreeing to our use of cookies. Privacy Statement

Get in Touch with Dako in Switzerland

Contact Us Directly
+41 41 760 11 66
+41 41 760 11 77

Pathology Products, Flow Cytometry Products and Specific Proteins
Dako Schweiz GmbH

Grabenstrasse 27
Postfach 1036
CH-6341 Baar

Detailed contact information

Submit an Inquiry

How would you like us to follow up?

Your session expired

You have been logged out. Log in again to retrieve your Cart.