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Monoclonal Mouse Anti-Human
Cytokeratin 20
Clone Ks20.8
Reacts with the 46 kDa protein corresponding to cytokeratin 20.
The antibody labels the majority of adenocarcinomas of the colon, mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas, and frequently adenocarcinomas of the stomach, bile system and pancreas. Most squamous cell carcinomas and most adenocarcinomas from other sites (breast, endometrium, lung, prostate), non-mucinous tumors of the ovary, and small-cell lung carcinomas are essentially or completely negative.
- Moll R, Löwe A, Laufer J, Franke WW. Cytokeratin 20 in human carcinomas. Am J Pathol 1992;140:427-47.
- Wauters CCAP, Smedts F, Gerrits LGM, Bosman FT, Ramaekers FCS. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary. Hum Pathol 1995;26:852-5.
- Harnden P, Mahmood N, Southgate J. Expression of cytokeratin 20 redefines urothelial papillomas of the bladder. Lancet 1999;353:974-7.
- Chu P, Wu W, Weiss LM. Cytokeratin 7 and cytokeratin 20 expression in epithelial neoplasms: a survey of 435 cases. Mod Pathol 2000;13:962-72.
Colon adenocarcinoma (FFPE) stained with FLEX Anti-Cytokeratin 20, Code IR777/IS777.
Colon adenocarcinoma. The majority of the neoplastic cells show a strong cytoplasmic staining reaction.
Clinical application for IR777/IS777. Primarily for identification of gastrointestinal tumors, mucinous ovarian tumors and Merkel cell carcinoma. Anti-Cytokeratin 20 stains the normal gastrointestinal epithelium and urothelium.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
FLEX Monoclonal Mouse Anti-Human Cytokeratin 20, Clone Ks20.8, Ready-to-Use, (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels normal and abnormal gastric and intestinal epithelium, urothelium and Merkel cells (1, 2), and this antibody is of particular value in the classification of carcinomas originating from these cell types (1, 3-5). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Clone: Ks20.8 (1). Isotype: IgG2a, kappa.
- Moll R, Löwe A, Laufer J, Franke WW. Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol 1992; 140:427-47.
- Moll R. Cytokeratins as markers of differentiation in the diagnosis of epithelial tumors. In: Herrmann, Harris, editors. Subcellular biochemistry. Volume 31, New York: Plenum Press; 1998. p. 205-62.
- Savera AT, Torres FX, Linden MD, Bacchi CE, Gown AM, Zarbo RJ. Primary versus metastatic pulmonary adenocarcinoma. An immunohistochemical study using villin and cytokeratins 7 and 20. Appl Immunohistochem 1996; 4:86-94.
- Harnden P, Mahmood N, Southgate J. Expression of cytokeratin 20 redefines urothelial papillomas of the bladder. Lancet 1999; 353:974-77.
- Wauters CCAP, Smedts F, Gerrits LGM, Bosman FT, Ramaekers FCS. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary. Hum Pathol 1995; 26:852-55.
| Product | Size |  | IS777 Clone Ks20.8
| 30 tests, 6 mL Product No. IS77730 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Colon | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
Synonym(s) for Antigen
protein IT