B-Cell-Specific Activator Protein
B-cell-specific activator protein, BSAP, also known as Pax-5, is a transcription factor expressed in B cells.
Antibodies to BSAP may be useful for the identification of pro, pre, and mature B cells and for classification of lymphomas and subclassification of classic Hodgkin's lymphoma and anaplastic large cell lymphoma of the T and null-cell type.
Hodgkin's lymphoma (FFPE) stained with FLEX Anti-BSAP, Code IR650/IS650.
- Optimized staining performance of both high and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains
- a guide to diagnostic accuracy.
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human B-Cell-Specific Activator Protein, Clone DAK-Pax5, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. Antibodies to B-cell-specific activator protein (BSAP) may be useful for the identification of pro-, pre-, and mature B cells and in the classification of lymphomas (1-4). Together with a panel of antibodies it is particularly useful in the differential identification of classic Hodgkin's disease versus anaplastic large cell lymphoma of T- and null-cell type (1, 3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Synthetic 17-meric peptide from the C-terminal inhibitory domain of the protein.
In Western blotting of mouse spleen cell lysate, the antibody labels a 55 kDa protein corresponding to BSAP.
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: DAK-Pax5. Isotype: IgG1, kappa.
- Browne P, Petrosyan K, Hernandez A, Chan JA. The B-cell transcription factors BSAP, Oct-2, and BOB.1 and the pan-B-cell markers CD20, CD22 and CD79a are useful in the differential diagnosis of classic Hodgkin lymphoma. Am J Clin Pathol 2003;120:767-77.
- Krenacs L, Himmelmann AW, Quintanilla-Martinez L, Fest T, Riva A, Wellmann A, et al. Transcription factor B-cell-specific activator protein (BSAP) is differentially expressed in B cells and in subsets of B-cell lymphomas. Blood 1998;92:1308-16.
- Torlakovic E, Torlakovic G, Nguyen PL, Brunning RD, Delabie J. The value of anti-pax-5 immunostaining in routinely fixed and paraffin-embedded sections: a novel pan pre-B and B-cell marker. Am J Surg Pathol 2002;26:1343-50.
- Zhang X, Lin Z, Kim I. Pax5 expression in non-Hodgkin's lymphomas and acute leukemias. J Korean Med Sci 2003;18:804-8.