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Monoclonal Mouse Anti-Human
Collagen IV
Clone CIV 22
Is directed against collagen IV, a major constituent of the basement membrane. The antibody is important in demonstrating the loss of basement membrane components by invasive carcinomas.
Isotype IgG1, kappa
- Frozen
- Formalin
- HIER
References
- McDermott MB, Swanson PE, Wick MR. Immunostains for collagen type IV discriminate between C-cell hyperplasia and microscopic medullary carcinoma in multiple endocrine neoplasia, type 2a. Hum Pathol 1995;26:1308-12.
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human Collagen IV, Clone CIV 22, is intended for use in immunocytochemistry. The antibody labels type IV collagen and is a useful tool for the identification of basement membranes. Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Human Collagen IV, Clone CIV 22, is intended for use in immunocytochemistry. The antibody labels type IV collagen and is a useful tool for the identification of basement membranes. Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Purified pepsin fragments of type IV collagen isolated from human kidney (2).
Specificity
The specificity of the antibody has been assessed in radioimmunoassay (RIA) where it recognizes an epitope present in the human type IV collagen in native conformation, whereas it does not recognize the reduced and alkylated protein in the denatured state. Staining of immunoblots with the antibody was negative, further indicating that the antibody recognizes a conformational epitope on type IV collagen (2).
In immunoblotting or RIA, no cross-reactivity of the antibody with isolated human collagen types I, II, III and V could be detected (2).
As demonstrated by RIA, the antibody cross-reacts with the collagen IV-equivalent protein in cow (2).
In immunoblotting or RIA, no cross-reactivity of the antibody with isolated human collagen types I, II, III and V could be detected (2).
As demonstrated by RIA, the antibody cross-reacts with the collagen IV-equivalent protein in cow (2).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 50 mmol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: CIV 22 (2). Isotype: IgG1, kappa.
IgG concentration: see label on vial.
Clone: CIV 22 (2). Isotype: IgG1, kappa.
IgG concentration: see label on vial.
References
- Odermatt BF, Lang AB, Rüttner JR, Winterhalter KH, Trüeb B. Monoclonal antibodies to human type IV collagen. Useful reagents to demonstrate the heterotrimeric nature of the molecule. Proc Natl Acad Sci 1984;81:7343-47.
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| Product | Size |  | M0785 Culture supernatant
| 1 mL Product No. M078501-2 |
|---|