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Monoclonal Mouse Anti-Human
EGFR
Clone H11
Labels the majority of simple and squamous epithelia. EGFR overexpression has been demonstrated in a variety of neoplasms, for example in a proportion of cases of breast, bladder, pancreatic, stomach and thyroid tumors.
Isotype IgG1, kappa
- Frozen
- Formalin
- Enzyme
Squamous cell carcinoma (FFPE) stained with Anti-EGFR.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Epidermal Growth Factor Receptor (anti-EGFR) is intended for laboratory use to identify qualitatively by light microscopy epidermal growth factor receptor positive cells in normal and neoplastic tissues using immunohistochemical (IHC) test methods. The clinical interpretation of any positive staining or its absence should be complemented by morphological and histological studies with proper controls. Evaluations should be made within the context of the patient's clinical history and other diagnostic tests by a qualified individual.
Monoclonal Mouse Anti-Epidermal Growth Factor Receptor (anti-EGFR) is intended for laboratory use to identify qualitatively by light microscopy epidermal growth factor receptor positive cells in normal and neoplastic tissues using immunohistochemical (IHC) test methods. The clinical interpretation of any positive staining or its absence should be complemented by morphological and histological studies with proper controls. Evaluations should be made within the context of the patient's clinical history and other diagnostic tests by a qualified individual.
Immunogen
A combination immunization protocol was employed using microsomal membrane preparations of HC2 20 d2 xenograft cells and a 14 amino acid EGFRvIII-specific synthetic peptide, Pep 3 conjugated to KLH (1).
Specificity
In Western blots of A431 cell extracts, monoclonal anti-human EGFR, clone H11 (anti-EGFR) recognizes the Mr 170 kD wild-type EGFR and the Mr 145 kD deletion mutant form of the receptor (EGFRvIII). In an analytical flow cytometric assay, anti-EGFR was demonstrated to strongly label the surface of a homogeneous population of A431 cells which overexpress the normal EGFR by a factor of 20-50 fold. Anti-EGFR also positively stained cells from the EGFRvIII-transfected HC2 20 d2 cell line (1).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as tissue culture supernatant in 0.05 mol/L Tris-HCl, pH 7.2 and 0.015 mol/L sodium azide. This product contains stabilizing protein.
Clone: H11. Isotype: IgG1, kappa
Mouse lgG concentration mg/L: See label on vial.
Clone: H11. Isotype: IgG1, kappa
Mouse lgG concentration mg/L: See label on vial.
References
- Wikstrand CJ, Hale LP, Batra SK, Hill ML, Humphrey PA, Kurpad SN, McLendon RE, Moscatello D, Pegram CN, Reist CJ, Traweek ST, Wong AJ, Zulatsky MR, Bigner DD. Monoclonal antibodies against EGFRvIII are tumor specific and react with breast and lung carcinomas and malignant gliomas. Canc Res 1995;55(14):3140-8
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| Product | Size |  | M3563 Culture supernatant
| 1 mL Product No. M356301-2 |
|---|
Synonym(s) for Antigen
epidermal growth factor receptor, erbB-1, HER1 protein