The antigen used for immunization is the C-terminal half (Mr 23000) of chromogranin A isolated from urine of patients with carcinoid tumors.
A reference to the use of anti-chromogranin in the study of tumors of neuroendocrine origin is (1).
- Kimura N, Sasano N, Yamada R, Satoh J. Immunohistochemical study of chromogranin in 100 cases of pheochromocytoma, carotid body tumour, medullary thyroid carcinoma, and carcinoid tumour. Virch Arch [A] 1988;413:33-8.
Small cell lung carcinoma (FFPE) stained with FLEX Anti-Chromogranin A, Code IR502/IS502.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains
- a guide to diagnostic accuracy
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Human Chromogranin A, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels chromogranin A present in secretory granules of endocrine cells (1). Chromogranin A is a marker for neuroendocrine differentiation and antibodies to chromogranin A are useful for identification of neuroendocrine-derived tumors. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Chromogranin A isolated from urine of patients with carcinoid syndrome.
The antibody reacts with human chromogranin A, but not with epitopes located on the N-terminal half of thechromogranin A molecule. The antibody labels the neuroendocrine cells and neural tissue in colon, pancreas and peripheral nerve tissue. No non-specific staining has been observed. Traces of contaminating antibodies have been removed by solid-phase absorption with human urine proteins.
As demonstrated by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections, the antibody cross-reacts with the chromogranin A-equivalent protein in cow, horse and swine.
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
- Moorghen M, Carpenter F. Peanut lectin: a histochemical marker for phaeochromocytomas. Virchows Arch A Pathol Anal Histopathol 1991; 419:203-7.
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