Reacts with the 46 kDa protein corresponding to cytokeratin 20.
The antibody labels the majority of adenocarcinomas of the colon, mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas, and frequently adenocarcinomas of the stomach, bile system and pancreas. Most squamous cell carcinomas and most adenocarcinomas from other sites (breast, endometrium, lung, prostate), non-mucinous tumors of the ovary, and small-cell lung carcinomas are essentially or completely negative.
- Moll R, Löwe A, Laufer J, Franke WW. Cytokeratin 20 in human carcinomas. Am J Pathol 1992;140:427-47.
- Wauters CCAP, Smedts F, Gerrits LGM, Bosman FT, Ramaekers FCS. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary. Hum Pathol 1995;26:852-5.
- Harnden P, Mahmood N, Southgate J. Expression of cytokeratin 20 redefines urothelial papillomas of the bladder. Lancet 1999;353:974-7.
- Chu P, Wu W, Weiss LM. Cytokeratin 7 and cytokeratin 20 expression in epithelial neoplasms: a survey of 435 cases. Mod Pathol 2000;13:962-72.
Merkel cell carcinoma (FFPE) stained with FLEX Anti-Cytokeratin 20, Code IR777/IS777.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains
- a guide to diagnostic accuracy
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Cytokeratin 20, Clone Ks20.8, Ready-to-Use, (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels normal and abnormal gastric and intestinal epithelium, urothelium and Merkel cells (1, 2), and this antibody is of particular value in the classification of carcinomas originating from these cell types (1, 3-5). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
CK20 isolated by SDS-PAGE from cytoskeletal material obtained from human duodenal mucosa (1).
In Western immunoblotting of SDS-PAGE-separated cytoskeletal proteins from human duodenal mucosa, the antibody labels a 46 kDa polypeptide corresponding to CK20 (1).
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: Ks20.8 (1). Isotype: IgG2a, kappa.
- Moll R, Löwe A, Laufer J, Franke WW. Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol 1992; 140:427-47.
- Moll R. Cytokeratins as markers of differentiation in the diagnosis of epithelial tumors. In: Herrmann, Harris, editors. Subcellular biochemistry. Volume 31, New York: Plenum Press; 1998. p. 205-62.
- Savera AT, Torres FX, Linden MD, Bacchi CE, Gown AM, Zarbo RJ. Primary versus metastatic pulmonary adenocarcinoma. An immunohistochemical study using villin and cytokeratins 7 and 20. Appl Immunohistochem 1996; 4:86-94.
- Harnden P, Mahmood N, Southgate J. Expression of cytokeratin 20 redefines urothelial papillomas of the bladder. Lancet 1999; 353:974-77.
- Wauters CCAP, Smedts F, Gerrits LGM, Bosman FT, Ramaekers FCS. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary. Hum Pathol 1995; 26:852-55.
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Synonym(s) for Antigen