Reacts with all red cells and their precursors and is well-suited for the demonstration of neoplasms derived from the erythroid lineage.
Isotype IgG1, kappa
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
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For in vitro diagnostic use.
Monoclonal Mouse Anti-Human Glycophorin C, Clone Ret40f, is intended for use in immunocytochemistry. The antibody labels erythrocytes and their precursors and is a useful tool for the demonstration of neoplasms derived from the erythroid linage (1, 2). Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
In Western blotting of normal erythrocyte membranes, the antibody labels a major band of 40 kDa corresponding to GPC and a number of bands of higher molecular weight presumed to represent complexes containing GPC. Erythrocyte membranes from the Leach phenotype are not labelled by the antibody (4).
In agglutination assay, the antibody agglutinates normal red blood cells, but does not react with red blood cells of the Leach phenotype, nor does the antibody react with sialidase-treated red blood cells, indicating a sialic acid-dependent epitope (4).
In smears of peripheral blood and bone marrow, the antibody displays labelling restricted to red blood cells and their precursors (4).
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: Ret40f (4). Isotype: IgG1, kappa.
Mouse IgG concentration: See label on vial.
- Thiele J, Kvasnicka HM, Diehl V, Fischer R, Michiels JJ. Clinicopathological diagnosis and differential criteria of thrombocythemias in various myeloproliferative disorders by histopathology, histochemistry and immunostaining from bone marrow biopsies. Leukemia Lymphoma 1999;207-18.
- Thiele J, Meuter RB, Titius RB, Zankovich R, Fischer R. Proliferating cell nuclear antigen expression by erythroid precursors in normal bone marrow, in reactive lesions and in polycythaemia rubra vera. Histopathology 1993;22:429-35.
- Gatter KC, Cordell JL, Turley H, Heryet A, Kieffer N, Anstee DJ, Mason DY. The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens. Histopathology 1988:13:257-67.
Synonym(s) for Antigen
CD235a, CD236R, glycophorin b