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Polyclonal Rabbit Anti-Human
Myeloperoxidase
The antigen has been isolated from primary granulocytes (1). The antibody reacts with neutrophil granulocytes and monocytes in blood and with precursors of granulocytes in the bone marrow.
The antibody is valuable for discrimination between lymphoid and myeloid leukemias and as an aid in the classification of neoplastic tissue, i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma (2) .
- Formalin
- Enzyme/HIER
References
- Matheson NR, Wong PS, Travis J. Isolation and properties of human neutrophil myeloperoxidase. Biochem 1981;20:325-30.
- Pinkus GS, Pinkus JI. Myeloperoxidase: a specific marker for myeloid cells in paraffin sections. Mod Pathol 1991;4:733-41.
Acute myeloid leukemia (FFPE) stained with FLEX Anti-Myeloperoxidase, Code IR511/IS511.
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Acute myeloid leukemia (M4). A strong granular cytoplasmic staining reaction is seen in part of the neoplastic cells, and a weak or absent staining is observed in the monocytes.
Clinical application for IR511/IS511 For differential diagnosis between lymphoid and myeloid tumors as myeloid tumors are generally positive and lymphoid tumors negative.
Liver. The neutrophils and Kupffer cells show a moderate to strong staining reaction. Hepatocytes should be negative or show a weak staining reaction.
Recommended control for IR511/IS511. Liver/Tonsil.
Tonsil. The neutrophils and eosinophils show a moderate to strong granular cytoplasmic staining reaction.
Recommended control for IR511/IS511. Liver/Tonsil.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Polyclonal Rabbit Anti-Human Myeloperoxidase is intended for use in immunohistochemistry. The antibody labels myeloperoxidase-positive cells in normal and neoplastic tissues and is a useful tool for the discrimination between lymphoid and myeloid disorders. However it is important that the antibody is not used alone but together with a panel of other antibodies (1, 2). Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Polyclonal Rabbit Anti-Human Myeloperoxidase is intended for use in immunohistochemistry. The antibody labels myeloperoxidase-positive cells in normal and neoplastic tissues and is a useful tool for the discrimination between lymphoid and myeloid disorders. However it is important that the antibody is not used alone but together with a panel of other antibodies (1, 2). Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Immunogen
Myeloperoxidase isolated from human polymorphonuclear leucocytes.
Specificity
The antibody reacts with human myeloperoxidase.
In crossed immunoelectrophoresis using 12.5 µL antibody per cm2 gel area against 2 µL myeloperoxidase only one precipitate corresponding to myeloperoxidase appears. Staining: Specific staining for peroxidase. With 2 µL of human plasma, no precipitate appears. Staining: Coomassie Brilliant Blue.
In formalin-fixed, paraffin-embedded human tonsil, and when using a sensitive streptavidin-biotin-based visualization system, the antibody as expected labels only rare neutrophil granulocytes.
As demonstrated by immunocytochemistry, the antibody cross-reacts with the myeloperoxidase-equivalent protein in cow, horse, rat and swine.
In crossed immunoelectrophoresis using 12.5 µL antibody per cm2 gel area against 2 µL myeloperoxidase only one precipitate corresponding to myeloperoxidase appears. Staining: Specific staining for peroxidase. With 2 µL of human plasma, no precipitate appears. Staining: Coomassie Brilliant Blue.
In formalin-fixed, paraffin-embedded human tonsil, and when using a sensitive streptavidin-biotin-based visualization system, the antibody as expected labels only rare neutrophil granulocytes.
As demonstrated by immunocytochemistry, the antibody cross-reacts with the myeloperoxidase-equivalent protein in cow, horse, rat and swine.
Reagent Provided
Purified immunoglobulin fraction of rabbit antiserum provided in liquid form. In 0.1 mol/L NaCl, 15 mmol/L NaN3.
Protein concentration g/L: See label on vial.
Protein concentration g/L: See label on vial.
References
- Arber DA, Jenkins KA. Paraffin section immunophenotyping of acute leukemias in bone marrow specimens. Am J Clin Pathol 1996;106:462-8.
- Arber DA, Snyder DS, Fine M, Dagis A, Niland J, Slovak ML. Myeloperoxidase immunoreactivity in adult acute lymphoblastic leukemia. Am J Clin Pathol 2001;116:25-33.
| Product | Size |  | A0398 Ig fraction
| 0.2 mL Product No. A039829 |
|---|
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Human Myeloperoxidase, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. The antibody labels myeloperoxidase-positive cells in normal and neoplastic tissues and is a useful tool for the discrimination between lymphoid and myeloid disorders. Positive results aid in the classification of neoplastic tissue (i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma) (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Polyclonal Rabbit Anti-Human Myeloperoxidase, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. The antibody labels myeloperoxidase-positive cells in normal and neoplastic tissues and is a useful tool for the discrimination between lymphoid and myeloid disorders. Positive results aid in the classification of neoplastic tissue (i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma) (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Myeloperoxidase isolated from human polymorphonuclear leucocytes.
Specificity
The antibody reacts with human myeloperoxidase. In crossed immunoelectrophoresis using 12.5 µL concentrated antibody per cm2 gel area against 2 µL myeloperoxidase only one precipitate corresponding to myeloperoxidase appears. Staining: Specific staining for peroxidase. With 2 µL of human plasma, no precipitate appears. Staining: Coomassie Brilliant Blue.
In formalin-fixed, paraffin-embedded human tonsil, and when using a sensitive streptavidin-biotin-based visualization system, the antibody as expected labels only rare neutrophil granulocytes.
In formalin-fixed, paraffin-embedded human tonsil, and when using a sensitive streptavidin-biotin-based visualization system, the antibody as expected labels only rare neutrophil granulocytes.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
References
- Pinkus GS, Pinkus JI. Myeloperoxidase: A specific marker for myeloid cells in paraffin sections. Mod Pathol 1991; 4:733
| Product | Size | | IR511 RTU, FLEX
| 60 tests, 12 mL Product No. IR51161 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IR600) Positive tissue: Tonsil and liver | 120 tests, 24 mL Product No. IR60066 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Human Myeloperoxidase, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. The antibody labels myeloperoxidase-positive cells in normal and neoplastic tissues and is a useful tool for the discrimination between lymphoid and myeloid disorders. Positive results aid in the classification of neoplastic tissue (i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma) (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Polyclonal Rabbit Anti-Human Myeloperoxidase, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. The antibody labels myeloperoxidase-positive cells in normal and neoplastic tissues and is a useful tool for the discrimination between lymphoid and myeloid disorders. Positive results aid in the classification of neoplastic tissue (i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma) (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Myeloperoxidase isolated from human polymorphonuclear leucocytes.
Specificity
The antibody reacts with human myeloperoxidase. In crossed immunoelectrophoresis using 12.5 µL concentrated antibody per cm2 gel area against 2 µL myeloperoxidase only one precipitate corresponding to myeloperoxidase appears. Staining: Specific staining for peroxidase. With 2 µL of human plasma, no precipitate appears. Staining: Coomassie Brilliant Blue.
In formalin-fixed, paraffin-embedded human tonsil, and when using a sensitive streptavidin-biotin-based visualization system, the antibody as expected labels only rare neutrophil granulocytes.
In formalin-fixed, paraffin-embedded human tonsil, and when using a sensitive streptavidin-biotin-based visualization system, the antibody as expected labels only rare neutrophil granulocytes.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
References
- 1. Pinkus GS, Pinkus JI. Myeloperoxidase: A specific marker for myeloid cells in paraffin sections. Mod Pathol 1991; 4:733
| Product | Size | | IS511 RTU, FLEX
| 30 tests, 6 mL Product No. IS51130 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IS600) Positive tissue: Tonsil and liver | 60 tests, 12 mL Product No. IS60061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |