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Polyclonal Rabbit Anti-Human
Progesterone Receptor
A carefully selected synthetic peptide from human progesterone receptor has been used for immunization. The antibody strongly labels the nuclei of cells known to contain a high level of progesterone receptor, e.g. normal, hyperplastic and neoplastic cells of the mammary gland, as well as epithelial and myometrial cells of the uterus. The antibody is very useful in conjunction with Dako Anti-Human Estrogen Receptor α.
- Frozen
- Formalin
- HIER
- Excellent specificity
- High lot to lot consistency
- Certified manufacturing facilities guarantee full quality control
Intended Use
Analyte specific reagent. Analytical and performance characteristics are not established.
Progesterone receptor (PR) is a member of the steroid receptor superfamily that includes receptors for several steroids, thyroid hormone, oxysterols, and fat-soluble vitamins. PR exits in two isoforms, PRA and PRB, that are expressed from a single gene as a result of transcription from two alternative promoters (1). Isoform PRB contains an N-terminal fragment of 164 amino acids that is not present in isoform PRA (2). Binding of progesterone to PR induces a conformational change resulting in dimerization of two ligand receptor complexes, increased receptor phosphorylation, and binding of receptor dimers to specific hormone-responsive DNA elements (1).
Progesterone receptor (PR) is a member of the steroid receptor superfamily that includes receptors for several steroids, thyroid hormone, oxysterols, and fat-soluble vitamins. PR exits in two isoforms, PRA and PRB, that are expressed from a single gene as a result of transcription from two alternative promoters (1). Isoform PRB contains an N-terminal fragment of 164 amino acids that is not present in isoform PRA (2). Binding of progesterone to PR induces a conformational change resulting in dimerization of two ligand receptor complexes, increased receptor phosphorylation, and binding of receptor dimers to specific hormone-responsive DNA elements (1).
Immunogen
Synthetic peptide, GLPQVYPPYLNYLRP, corresponding to amino acids 533-547 in the N-terminal region of the progesterone receptor shared by both PR isoforms. The peptide has been coupled to ovalbumin.
Specificity
The antibody labels the progesterone receptor. Traces of contaminating antibodies have been removed by solid-phase absorption with ovalbumin.
In crossed immunoelectrophoresis, using 12.5 µL antibody per cm2 gel area against 2 µL of human plasma and 2 µL of ovalbumin, no reactions with plasma proteins and ovalbumin were observed. Staining: Coomassie Brilliant Blue.
In crossed immunoelectrophoresis, using 12.5 µL antibody per cm2 gel area against 2 µL of human plasma and 2 µL of ovalbumin, no reactions with plasma proteins and ovalbumin were observed. Staining: Coomassie Brilliant Blue.
Reagent Provided
Purified immunoglobulin fraction of rabbit antiserum provided in liquid form in 0.1 mol/L NaCl, 15 mmol/L NaN3.
Protein concentration g/L: See label on vial.
Protein concentration g/L: See label on vial.
References
- Conneely OM, Mulac-Jericevic B, Lydon JP. Progesterone-dependent regulation of female reproductive activity by two distinct progesterone receptor isoforms. Steroids 2003;68:771-8.
- Milliken EL, Ameduri RK, Landis MD, Behrooz A, Abdul-Karim FW, Keri RA. Ovarian hyperstimulation by LH leads to mammary gland hyperplasia and cancer predisposition in transgenic mice. Endocrinology 2002;143:3671-80.
| Product | Size |  | A0098 Ig fraction
| 1 mL Product No. A009801 |
|---|
Synonym(s) for Antigen
PR