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Polyclonal Rabbit Anti-Human
Lambda Light Chains
The antigen used for immunization is a pool of human lambda Bence Jones proteins. Therefore, a reagent with a particularly wide specificity for lambda-chains is obtained. The specificity is directed against surface as well as hidden determinants and has been ascertained by gel precipitation techniques and immunohistochemistry.
A0193 is excellent for the typing of free and bound monoclonal lambda-chains by immunoelectrophoresis and immunofixation.
- Precipitation
- Blot
- ELISA
- IHC
- Excellent specificity and precipitation ability
- High lot to lot consistency
- Optimized for a variety of applications with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Polyclonal Rabbit Anti-Human Lambda Light Chains, is intended for use in immunohistochemistry. The antibody is also well-suited for gel immunoprecipitation techniques, including immunofixation and immunoblotting (1). In immunocytochemistry, the antibody labels plasma cells and related lymphoid cells containing lambda light chains, and it is a useful tool for the classification of patients with monoclonal gammopathies (2) and amyloidosis (3, 4). Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of B cells (5-7). Differential identification is aided by the results from a panel of antibodies. Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Polyclonal Rabbit Anti-Human Lambda Light Chains, is intended for use in immunohistochemistry. The antibody is also well-suited for gel immunoprecipitation techniques, including immunofixation and immunoblotting (1). In immunocytochemistry, the antibody labels plasma cells and related lymphoid cells containing lambda light chains, and it is a useful tool for the classification of patients with monoclonal gammopathies (2) and amyloidosis (3, 4). Additionally, the antibody may be used for distinguishing neoplastic monoclonal proliferation from reactive hyperplasia of B cells (5-7). Differential identification is aided by the results from a panel of antibodies. Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Immunogen
Polyclonal immunoglobulin light chains of lambda type isolated from a pool of human sera.
Specificity
The antibody reacts with free lambda chains as well as lambda chains in intact immunoglobulin molecules. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins.
Specificity tests; staining with Coomassie Brilliant Blue:
Double immunodiffusion: No reaction with kappa or gamma chains is seen when using 15 µL of antibody against 15 µL of kappa IgG.
Crossed immunoelectrophoresis: Only lambda-related precipitates appear when using 12.5 µL antibody per square cm gel area against 2 µL human plasma or 2 µL of a pool ofconcentrated urines from patients with tubular proteinuria.
Cross-reaction with immunoglobulins from other species may occur. As demonstrated by immunocytochemistry, the antibody cross-reacts with immunoglobulins in dog, horse, pig and rat.
Specificity tests; staining with Coomassie Brilliant Blue:
Double immunodiffusion: No reaction with kappa or gamma chains is seen when using 15 µL of antibody against 15 µL of kappa IgG.
Crossed immunoelectrophoresis: Only lambda-related precipitates appear when using 12.5 µL antibody per square cm gel area against 2 µL human plasma or 2 µL of a pool ofconcentrated urines from patients with tubular proteinuria.
Cross-reaction with immunoglobulins from other species may occur. As demonstrated by immunocytochemistry, the antibody cross-reacts with immunoglobulins in dog, horse, pig and rat.
Reagent Provided
Purified immunoglobulin fraction of rabbit antiserum provided in liquid form. In 0.1 mol/L NaCl, 15 mmol/L NaN3.
Protein concentration g/L: See label on vial.
Antibody titre (SRI): 1600 mg/L (10).
As no international lambda reference preparation is available, a polyclonal lambda preparation (isolated from human IgG) has been used for the titre determination. The titre variation between different lots of A0193 is less than 10%. This is achieved by adjusting the titre of each individual lot to match the titre of an antibody reference preparation kept at -80 °C.
Protein concentration g/L: See label on vial.
Antibody titre (SRI): 1600 mg/L (10).
As no international lambda reference preparation is available, a polyclonal lambda preparation (isolated from human IgG) has been used for the titre determination. The titre variation between different lots of A0193 is less than 10%. This is achieved by adjusting the titre of each individual lot to match the titre of an antibody reference preparation kept at -80 °C.
References
- Withold W, Reinauer H. An immunoblotting procedure following agarose gel electrophoresis for detection of Bence Jones proteinuria compared with immunofixation and quantitative light chain determination. Eur J Clin Chem Clin Biochem 1995;33:135-8.
- Peterson LC, Brown BA, Crosson JT, Mladenovic J. Application of the immunoperoxidase technic to bone marrow trephine biopsies in the classification of patients with monoclonal gammopathies. Am J Clin Pathol 1986;85:688-93.
- Strøm EH, Fogazzi GB, Banfi G, Pozzi C, Mihatsch MJ. Light chain deposition disease of the kidney. Morphological aspects in 24 patients. Virchows Arch 1994;425:271-80.
- Jessup E. Antigen retrieval techniques for the demonstration of immunoglobulin light chains in formalin-fixed, paraffin-wax embedded sections. UK NEQAS immunocytochemistry news. Winter 1994/95; Issue 4:12-6.
- Taylor CR, Burns J. The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody. J Clin Path 1974;27:14-20.
- Harris NL, Poppema S, Data RE. Demonstration of immunoglobulin in malignant lymphomas. Use of an immunoperoxidase technic on frozen sections. Am J Clin Pathol1982;78:14-21.
- Marshall-Taylor CE, Cartun RW, Mandich D, DiGuiseppe JA. Immunohistochemical detection of immunoglobulin light chain expression in B-cell non-Hodgkin lymphomas using formalin-fixed, paraffin-embedded tissues and a heat-induced epitope retrieval technique. Appl Immunohistochem Mol Morphol 2002;10:258-62.
- Becker W. Determination of antisera titres using the single radial immunodiffusion method. Immunochem 1969;6:539-46.
| Product | Size |  | A0193 Ig fraction
| 2 mL Product No. A019302 | | F0199 FITC. Ig fraction
| 2 mL Product No. F019902 |
|---|