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Monoclonal Rabbit Anti-Human
Estrogen Receptor α
Clone EP1
This monoclonal rabbit antibody reacts with human estrogen receptor α (ER α).
Estrogens have been found to be preferentially concentrated in the estrogen target organs of animals and in human breast cancers, and it is well documented that the mitogenic effects of estrogen are mediated by ER. Historical studies have shown that ER status is correlated with untreated outcome (i.e. prognostic for well-differentiated invasive breast cancer) and with response to anti-hormonal therapy, e.g. tamoxifen (1). The antibody may be used in the semi-quantitative detection of human estrogen receptor in tissue sections of human breast cancer by immunohistochemistry.
For DakoLink users:
Go to the Autostainer Link Installer section and download the Installer for Code IR084, Estrogen Receptor α, Clone EP1 Protocol Updater. Follow the instructions to complete the update.
- Formalin
- HIER
References
- Elledge RM, Fuqua SAW. Chapter 31: Estrogen and Progesterone Receptors. In: Diseases of the Breast. Harris JR, et al. eds. Philadelphia: Lippincott Williams & Wilkins 2000:471-85.
Breast carcinoma (FFPE) stained with FLEX Anti-Estrogen Receptor α, Clone EP1.
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Breast carcinoma with positive estrogen receptor expression. The neoplastic cells show a moderate to strong staining reaction.
Clinical application for Anti-ER α, Clone EP1. For assessment of estrogen receptor status in breast carcinomas.
Breast tissue with positive estrogen receptor expression. The neoplastic cells show a moderate to strong staining reaction.
Clinical application for Anti-ERα, Clone EP1. For assessment of estrogen receptor status in breast carcinomas.
Breast tissue with positive estrogen receptor expression. The neoplastic cells show a moderate to strong staining reaction.
Clinical application for Anti-ERα, Clone EP1. For assessment of estrogen receptor status in breast carcinomas.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Rabbit Anti- Human Estrogen Receptor α, Clone EP1, is intended for use in immunohistochemistry to semi-quantitatively detect human estrogen receptor in formalin-fixed, paraffin-embedded tissue sections of human breast cancer. The antibody labels estrogen receptor α-positive cells and is useful in the assessment of estrogen receptor status in human breast carcinomas.
The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Rabbit Anti- Human Estrogen Receptor α, Clone EP1, is intended for use in immunohistochemistry to semi-quantitatively detect human estrogen receptor in formalin-fixed, paraffin-embedded tissue sections of human breast cancer. The antibody labels estrogen receptor α-positive cells and is useful in the assessment of estrogen receptor status in human breast carcinomas.
The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Recombinant protein of ER α amino acids 1-300.
Specificity
In Western blotting of MCF7 cells lysates the antibody labels a major band of approximately 67 kDa, corresponding to the expected molecular weight of ER α. No cross-reactivity was seen with ER β.
Reagent Provided
Monoclonal rabbit antibody provided in liquid form as affinity-isolated antibody in 0.01 mol/L phosphate buffered saline, pH 7.2, and 0.09% sodium azide. This product contains stabilizing protein.
Clone: EP1.
Rabbit IgG concentration: See label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot
Clone: EP1.
Rabbit IgG concentration: See label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot
| Product | Size |  | M3643 Affinity isolated
| 0.2 mL Product No. M364329 |
|---|---|---|
| 1 mL Product No. M364301 |
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Antibody Diluent (Code S0809) Dilute 1:40 | 50 mL Product No. S080981 |
| 125 mL Product No. S080983 | ||
| Negative Control | Rabbit Immunoglobulin Fraction (Solid-Phase Absorbed) (Code X0936) Positive tissue: Low ER-expressing breast carcinoma tissue | 2 mL Product No. X093602 |
| Visualization System | EnVision™ FLEX (Code K8000/K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Code K8008/K8018) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | Silanized Slides (Code S3003) Recommended for greater adherence to glass slides | 100 slides Product No. S300330 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Clone EP1, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments to semi-quantitatively detect human estrogen receptor in formalin-fixed, paraffin-embedded tissue sections of human breast cancer. The antibody labels estrogen receptor α-positive cells and is useful in the assessment of estrogen receptor status in human breast carcinomas.
The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist
FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Clone EP1, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments to semi-quantitatively detect human estrogen receptor in formalin-fixed, paraffin-embedded tissue sections of human breast cancer. The antibody labels estrogen receptor α-positive cells and is useful in the assessment of estrogen receptor status in human breast carcinomas.
The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist
Immunogen
Recombinant protein of ER α amino acids 1-300.
Specificity
In Western blotting of MCF7 cells lysates the antibody labels a major band of approximately 67 kDa, corresponding to the expected molecular weight of ER α. No cross-reactivity was seen with ER β.
Reagent Provided
Ready-to-use monoclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: EP1.
Clone: EP1.
| Product | Size | | IR084 RTU, FLEX
| 60 tests, 12 mL Product No. IR08461 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IR600) Positive tissue: Low ER-expressing breast carcinoma tissue or benign cervix | 120 tests, 24 mL Product No. IR60066 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | Silanized Slides (Code S3003) Recommended for greater adherence Alternative is FLEX IHC Microscope Slides (Code K8020) | 100 slides Product No. S300330 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Clone EP1, Ready-to-Use (Dako Autostainer/ Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments to semi-quantitatively detect human estrogen receptor in formalin-fixed, paraffin-embedded tissue sections of human breast cancer. The antibody labels estrogen receptor α-positive cells and is useful in the assessment of estrogen receptor status in human breast carcinomas
The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Clone EP1, Ready-to-Use (Dako Autostainer/ Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments to semi-quantitatively detect human estrogen receptor in formalin-fixed, paraffin-embedded tissue sections of human breast cancer. The antibody labels estrogen receptor α-positive cells and is useful in the assessment of estrogen receptor status in human breast carcinomas
The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Recombinant protein of ER α amino acids 1-300.
Specificity
In Western blotting of MCF7 cells lysates the antibody labels a major band of approximately 67 kDa, corresponding to the expected molecular weight of ER α. No cross-reactivity was seen with ER β.
Reagent Provided
Ready-to-use monoclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: EP1.
Clone: EP1.
| Product | Size | | IS084 RTU, FLEX
| 30 tests, 6 mL Product No. IS08430 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Rabbit (Code IS600) Positive tissue: As an alternative, benign cervix | 60 tests, 12 mL Product No. IS60061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | Silanized Slides (Code S3003) Recommended for greater adherence Alternative is FLEX IHC Microscope Slides (Code K8020) | 100 slides Product No. S300330 |
Synonym(s) for Antigen
ERα, ER alpha, ER