Secondary antibodies (antibodies to animal immunoglobulins) are utilized either for the direct detection of animal immunoglobulins or for amplification of the reaction between a primary antibody and the target antigen. Secondary antibodies play an important role in immunohistochemical procedures, immunoblotting, ELISAs and a number of other immunological methods. All Dako secondary antibodies and their conjugates are carefully tested to ensure good performance and low lot-to-lot variation. Several Dako secondary antibodies are presented in different grades regarding antiserum purification (immunoglobulin fraction of antiserum, affinity-isolated antibody, F(ab')₂ fragment antibody) and also regarding the absorptions which have been performed to remove interspecies cross-reactions.

Further Information
A package insert with detailed product description and guidelines for dilutions accompanies all Dako secondary antibodies.