Thyroglobulin is the precursor of thyroid hormones.
It is synthesized by thyrocytes and transported to the apical surface where it is secreted into the lumen of thyroid follicles and stored as the major component of colloid. The antibody is useful for the detection of thyroglobulin in thyroid tissue and is a useful aid for classification of well-differentiated thyroid carcinomas.
Thyroid follicular carcinoma (FFPE) stained with FLEX Anti-Thyroglobulin, Code IR509/IS509.
- Optimized staining performance of both high and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains
- a guide to diagnostic accuracy.
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Human Thyroglobulin, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of thyroglobulin in thyroid tissue and is a useful tool for the identification of well differentiated thyroid carcinomas (1, 2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Human thyroglobulin isolated from human thyroid glands.
The antibody labels human Tg, traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins.
In crossed immunoelectrophoresis using 12.5 µL concentrated antibody per cm2 gel area against 2 µL of human thyroid gland extract, only one precipitate corresponding to Tg appears. With 2 µL of human plasma, no precipitate appears. Staining: Coomassie Brilliant Blue.
In human thyroid cell cultures, the antibody labels intracellular Tg (4).
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
- Albores-Saavedra J, Nadji M, Civantos F, Morales AR. Thyroglobulin in carcinoma of the thyroid: An immunohistochemical study. Hum Pathol 1983;14:62-6.
- Burt AD, Kerr DJ, Brown IL, Boyle P. Lymphoid and epithelial markers in small cell anaplastic thyroid tumors. J Clin Pathol 1985;38:893-6.
- Kayser L, Olsen BE, Høyer PE. Quantitative cytochemical demonstration of intracellular thyroglobulin in cultured human thyrocytes. Effects of fixatives, TSH and interleukin-1a. Histochem J 1991;23:235-40