The antigen has been isolated from primary granulocytes (1). The antibody reacts with neutrophil granulocytes and monocytes in blood and with precursors of granulocytes in the bone marrow.
The antibody is valuable for discrimination between lymphoid and myeloid leukemias and as an aid in the classification of neoplastic tissue, i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma (2) .
- Matheson NR, Wong PS, Travis J. Isolation and properties of human neutrophil myeloperoxidase. Biochem 1981;20:325-30.
- Pinkus GS, Pinkus JI. Myeloperoxidase: a specific marker for myeloid cells in paraffin sections. Mod Pathol 1991;4:733-41.
Acute myeloid leukemia (FFPE) stained with FLEX Anti-Myeloperoxidase, Code IR511/IS511.
- Optimized staining performance of both high and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains
- a guide to diagnostic accuracy.
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Human Myeloperoxidase, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. The antibody labels myeloperoxidase-positive cells in normal and neoplastic tissues and is a useful tool for the discrimination between lymphoid and myeloid disorders. Positive results aid in the classification of neoplastic tissue (i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma) (1). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Myeloperoxidase isolated from human polymorphonuclear leucocytes.
The antibody reacts with human myeloperoxidase. In crossed immunoelectrophoresis using 12.5 µL concentrated antibody per cm2 gel area against 2 µL myeloperoxidase only one precipitate corresponding to myeloperoxidase appears. Staining: Specific staining for peroxidase. With 2 µL of human plasma, no precipitate appears. Staining: Coomassie Brilliant Blue.
In formalin-fixed, paraffin-embedded human tonsil, and when using a sensitive streptavidin-biotin-based visualization system, the antibody as expected labels only rare neutrophil granulocytes.
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
- Pinkus GS, Pinkus JI. Myeloperoxidase: A specific marker for myeloid cells in paraffin sections. Mod Pathol 1991; 4:733