Neuron-Specific Enolase (NSE)
Neuron-specific enolase is one of three main groups of enolases, the other two being non-neuronal enolase and muscle-specific enolase.
The antibody labels both normal and neoplastic cells of neuronal and neuroendocrine origin. Although neuron-specific enolase is not an exclusive neuronal marker, it may be used for the identification of peripheral nerves, neural and neuroendocrine tumors, such as neuroblastomas, retinoblastomas, desmoplastic malignant melanoma, and small-cell lung cancer.
Carcinoid tumor (FFPE) stained with FLEX Anti-NSE, Code IR612/IS612.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains
- a guide to diagnostic accuracy
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Neuron-Specific Enolase, Clone BBS/NC/VI-H14, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels both normal and neoplastic cells of neuronal and neuroendocrine origin (1), and although neuron-specific enolase (NSE) is not an exclusive neuronal marker, it may be used for the identification of peripheral nerves, neural and neuroendocrine tumors, such as neuroblastomas, retinoblastomas, desmoplastic malignant melanoma and small-cell lung cancer (2, 3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
γγ-enolase purified from human brain by a dissociation-reassociation method in a chromatofocusing column (1).
In Western blotting of reduced, SDS-denatured purified human γγ-enolase, αα-enolase, and extract of human muscle containing ββ-enolase, the antibody labels solely the γ-enolase subunit (1).
As demonstrated by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections, the antibody cross-reacts with the NSE-equivalent protein in pig (1).
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: BBS/NC/VI-H14 (1). Isotype: IgG1, kappa.
- Soler Federsppiel BS, Cras P, Gheuens J, Andries D, Lowenthal A. Human ((-enolase: two-site immuno-radiometric assay with a single monoclonal antibody. J Neurochem 1987;48:22-8.
- Anstey A, Cerio R, Ramnarain N, Orchard G, Smith N, Jones EW. Desmoplastic malignant melanoma. An immunocytochemical study of 25 cases. Am J Dermatopathol 1994;16:14-22.
- Kang J-L, Meyts ER, Skakkebæk NE. Immunoreactive neuron-specific enolase (NSE) is expressed in testicular carcinoma-in-situ. J Pathol 1996;178:161-5.
Synonym(s) for Antigen
14-3-2 protein, g-enolase, NSE