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Monoclonal Mouse Anti-Human
MUM1 Protein
Clone MUM1p
Labels MUM1 protein in a subset of light zone germinal centre (GC) B cells (probably centrocytes and their progeny), plasma cells and activated T cells.
It also labels and a wide spectrum of hematolymphoid neoplasms derived from these cells. Of non-hematolymphoid neoplasms only a proportion of melanomas are labeled (1, 2). Antibodies to MUM1 protein are useful for identifying the transition of GC B cells (BCL6+) to immunoblasts and plasma cells (CD138+), for the study of the histogenesis of B-cell lymphomas, and for the subclassification of these malignancies (1-3).
References
- Falini B, Fizzotti M, Pucciarini A, Bigerna B, Marafioti T, Gambacorta M, et al. A monoclonal antibody (MUM1p) detects expression of the MUM1/IRF4 protein in a subset of germinal center B cells, plasma cells, and activated T cells. Blood 2000;95;2084-92.
- Natkunam Y, Warnke RA, Montgomery K, Falini B, van de Rijn M. Analysis of MUM1/IRF4 protein expression using tissue microarrays and immunohistochemistry. Mod Pathol 2001;14:686-94.
- Gaidano G, Carbone A. MUM1: a step toward the understanding of lymphoma histogenesis. Leukemia 2000;14:563-6.
Classic Hodgkin's lymphoma, (FFPE) stained with FLEX Anti-MUM1 Protein, Code IR644/IS644.
Classic Hodgkin lymphoma. Reed-Sternberg cells show a nuclear staining reaction.
Clinical application for IR644/IS644 For subclassification of lymphoid malignancies.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human MUM1 protein, Clone MUM1p, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels the MUM1 protein, which is expressed in a subset of B cells in the light zone of the germinal centre (representing late stages of B-cell differentiation), plasma cells, activated T cells, and a wide spectrum of related haematolymphoid neoplasms. Of non-haematolymphoid neoplasms only a proportion of melanomas are labeled. The antibody is of value, for example, for the subclassification of lymphoid malignancies (1, 2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human MUM1 protein, Clone MUM1p, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels the MUM1 protein, which is expressed in a subset of B cells in the light zone of the germinal centre (representing late stages of B-cell differentiation), plasma cells, activated T cells, and a wide spectrum of related haematolymphoid neoplasms. Of non-haematolymphoid neoplasms only a proportion of melanomas are labeled. The antibody is of value, for example, for the subclassification of lymphoid malignancies (1, 2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Recombinant MUM1 fusion protein (1).
Specificity
Western blotting of pHeBo-CMV-MUM1-HA-transfected HeLa cell lysate, the antibody labels a band of 52 kDa corresponding to MUM1-HA protein, whereas no labeling is observed in non-transfected HeLa cell lysate. In Western blotting of lysates of IM9 (myeloma cell line), unfractionated tonsil cell suspensions, and PHA-stimulated T cells, the antibody labels a band of 50 kDa corresponding to MUM1 protein. No labeling is observed with lysates of U937 cells (myeloid cell line), and unstimulated T cells (1).
SDS-PAGE analysis of immunoprecipitates formed between lysate of the IM9 myeloma cell line and the antibody shows reaction with a 50 kDa protein corresponding to MUM1 protein (1).
SDS-PAGE analysis of immunoprecipitates formed between lysate of the IM9 myeloma cell line and the antibody shows reaction with a 50 kDa protein corresponding to MUM1 protein (1).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: MUM1p (1). Isotype: IgG1, kappa.
Clone: MUM1p (1). Isotype: IgG1, kappa.
References
- Falini B, Fizzotti M, Pucciarini A, Bigerna B, Marafioti T, Gambacorta M, et al. A monoclonal antibody (MUM1p) detects expression of the MUM1/IRF4 protein in a subset of germinal center B cells, plasma cells, and activated T cells. Blood 2000;95:2084-92.
- Natkunam Y, Warnke RA, Montgomery K, Falini B, van de Rijn M. Analysis of MUM1/IRF4 protein expression using tissue microarrays and immunohistochemistry. Mod Pathol 2001;14:686-94.
| Product | Size |  | IR644 Clone MUM1p
| 60 tests, 12 mL Product No. IR64461 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Colon/appendix and tonsil | 120 tests, 24 mL Product No. IR75066 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |