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Monoclonal Mouse Anti-
Epstein-Barr Virus, LMP
Clone CS.1-4
Labels the Epstein-Barr virus-encoded latent gene product, latent membrane protein (LMP).
The antibody can be used for the demonstration of latent EBV infection in cells and tissues. It has been found that EBV is associated with a high proportion of cases of Hodgkin's disease (1). LMP expression has also been found in nasopharyngeal carcinoma and non-lymphoblastic T-cell lymphoma (2, 3).
References
- Pallesen G, Hamilton-Dutoit SJ, Rowe M, Young LS. Expression of Epstein-Barr virus latent gene products in tumour cells of Hodgkins disease. Lancet 1991;337:320-2.
- Vera-Sempere FJ, Burgos JS, Botella MS, Cordoba J, Gobernado M. Immunohistochemical expression of Epstein-Barr virus-encoded latent membrane protein (LMP-1) in paraffin sections of EBV-associated nasopharyngeal carcinoma in Spanish patients. Oral Oncol, Eur J Cancer 1996;32B:163-8.
- dAmore F, Johansen P, Houmand A, Weisenburger DD, Mortensen LS. Epstein-Barr Virus genome in non-Hodgkins lymphomas occurring in immunocompetent patients: highest prevalence in nonlymphoblastic T-cell lymphoma and correlation with a poor prognosis. Blood 1996;87:1045-55.
Burkitt lymphoma (FFPE) stained with FLEX Anti-Epstein-Barr Virus, LMP, Code IR753/IS753.
Burkitt lymphoma/leukemia. Burkitt lymphoma/leukemia cells show a strong membranous and cytoplasmic staining reaction for Epstein-Barr virus.
Clinical application for IR753/IS753 For identification of Epstein-Barr virus-infected cells expressing the latent membrane protein (LMP-1) such as in Hodgkin lymphoma, Burkitt lymphoma/leukemia and nasopharyngeal carcinoma.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Epstein-Barr Virus, LMP, Clones CS.1-4, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. The antibody labels Epstein-Barr virus-infected cells expressing the latent membrane protein (LMP-1), and is a useful tool for the identification of tumor cells in Epstein-Barr virus-associated Hodgkin's disease (1-3) and nasopharyngeal carcinoma (4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Epstein-Barr Virus, LMP, Clones CS.1-4, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. The antibody labels Epstein-Barr virus-infected cells expressing the latent membrane protein (LMP-1), and is a useful tool for the identification of tumor cells in Epstein-Barr virus-associated Hodgkin's disease (1-3) and nasopharyngeal carcinoma (4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Recombinant fusion protein containing sequences of bacterial beta-galactosidase and the carboxyl half of EBV-encoded LMP (7).
Specificity
In Western blotting with lysates of EBV-transformed lymphoblastoid cell lines, the antibody labels a major band of 57-66 kDa depending on the virus isolate, sometimes accompanied by a minor band of 50-55 kDa corresponding to full length and truncated form of LMP. The antibody recognizes 20 geographically distinct EBV isolates (7). The antibody cross-reacts with a 43-44 kDa doublet of uncharacterized normal cell proteins in cellular extracts including that from EBV-negative control cell lines (7).
The antibody labels a normal EBV-positive lymphoblastoid cell line established by infection of normal resting B-cells with B95.8 virus, whereas the BL2 cell line, established from a rare EBV-negative sporadic BL-patient, as well as other unrelated cell isolates, including tonsillar B and T lymphocytes, fetal liver and thymus, cultured fetal fibroblasts, human leukaemic cell lines HSB2 and K562 and 7 EBV-negative B-cell lines show no labeling, except the cellular doublet proteins (7).
The antibody labels a normal EBV-positive lymphoblastoid cell line established by infection of normal resting B-cells with B95.8 virus, whereas the BL2 cell line, established from a rare EBV-negative sporadic BL-patient, as well as other unrelated cell isolates, including tonsillar B and T lymphocytes, fetal liver and thymus, cultured fetal fibroblasts, human leukaemic cell lines HSB2 and K562 and 7 EBV-negative B-cell lines show no labeling, except the cellular doublet proteins (7).
Reagent Provided
Ready-to-use mixture of four monoclonal mouse antibodies provided in liquid form as tissue culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2 containing 0.015 mol/L NaN3.
Clone: CS.1, CS.2, CS.3 and CS.4 Isotype: IgG1, kappa.
Clone: CS.1, CS.2, CS.3 and CS.4 Isotype: IgG1, kappa.
References
- Pallesen G, Hamilton-Dutoit SJ, Rowe M, Young LS. Expression of Epstein-Barr virus latent gene products in tumour cells of Hodgkin's disease. Lancet 1991;337:320-2.
- Murray PG, Young LS, Rowe M, Crocker J. Immunohistochemical demonstration of the Epstein-Barr virus-encoded latent membrane protein in paraffin sections of Hodgkin's disease. J Pathol 1992;166:1-5.
- Engel M, Essop MF, Close P, Hartley P, Pallesen G, Sinclair-Smith C. Improved prognosis of Epstein-Barr virus associated childhood Hodgkin's lymphoma: study of 47 South African cases. J Clin Pathol 2000;53:182-6.
- Vera-Sempere FJ, Burgos JS, Botella MS, Cordoba J, Gobernado M. Immunohistochemical expression of Epstein-Barr virus-encoded latent membrane protein (LMP-1) in paraffin sections of EBV-associated nasopharyngeal carcinoma in Spanish patients. Oral Oncol, Eur J Cancer 1996;32B:163-8.
- Rowe M, Evans HS, Young LS, Hennessy K, Kieff E, Rickinson AB. Monoclonal antibodies to the latent membrane protein of Epstein-Barr virus reveal heterogeneity of the protein and inducible expression in virus-transformed cells. J Gen Virol 1987;68:1575-86.
| Product | Size |  | IR753 Clones CS.1-4
| 60 tests, 12 mL Product No. IR75361 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: EBV-positive Burkitts and Hodgkin lymphoma | 120 tests, 24 mL Product No. IR75066 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |