Beta-catenin is an 88 kDa multifunctional protein playing an essential role in cell-cell adhesion by binding to the transmembrane protein, cadherin.
Beta-catenin is also involved in the regulation of gene expression as a mediator of the Wnt signaling pathway. The expression and intracellular localization of beta-catenin is altered in many types of cancers. Besides the reactivity with human beta-catenin, the M3539 antibody cross-reacts with mouse beta-catenin.
Colon adenoma (FFPE) stained with FLEX Anti-Beta-Catenin, Code IR702/IS702.
- Optimized staining performance of both high and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains
- a guide to diagnostic accuracy.
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Beta-Catenin, Clone β-catenin-1, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of desmoid tumors (1) and colorectal cancer (2). Antibodies to β-catenin may also be useful for the identification of colon adenoma (3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Recombinant C-terminal β-catenin fusion protein (7).
In Western blotting of human epithelial A431 cells the antibody labels a band corresponding to human β-catenin protein. No cross-reactivity with α and γ-catenin was observed (7).
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: β-catenin-1. Isotype: IgG1, kappa.
- Carlson JW, Fletcher CDM. Immunohistochemistry for ß-catenin in the differential diagnosis of spindle cell lesions: analysis of a series and review of the literature. Histopathol 2007;51:509-14.
- Lugli A, Zlobec I, Minoo P, Baker K, Tornillo L, Terracciano L, et al. Prognostic significance of the wnt signalling pathway molecules APC, ß-catenin and E-cadherin in colorectal cancer-a tissue microarray-based analysis. Histopathol. 2007;50:453-64.
- Iwamoto M, Ahnen DJ, Franklin WA, Maltzman TH. Expression of ß-catenin and full-length APC protein in normal and neoplastic colonic tissue. Carcinogenesis 2000;21:1935-40.
- Antibody Certification. On file at Dako.