Synthetic peptide from the intracellular part of the ε-chain of human CD3 was coupled to bovine thyroglobulin and used for immunization.
The antibody is a pan-T cell marker for detection of normal and neoplastic T cells. It is well-suited for detecting reactive T cells in tissue with lymphoid infiltrates, and for diagnosing T-cell neoplasms. The antibody cross-reacts with T cells in many animal species (1). In conjunction with Dako Monoclonal Mouse Anti-Human CD20cy (Code M0755), the antibody is well-suited for the differentiation between T and B cells in formalin-fixed tissue (2). Futhermore, antibodies against CD3 may aid in the differential diagnosis of Hodgkin's disease where CD3 is rarely expressed (3).
The antibody shows a stronger labeling intensity than corresponding monoclonal antibodies to CD3, and should, generally, be preferred on formalin-fixed, paraffin-embedded tissue sections.
- Jones M, Cordell JL, Beyers AD, Tse AGD, Mason DY. Detection of T and B cells in many animal species using cross-reactive antipeptide antibodies. J Immunol 1993;150:5429-35.
- Chadburn A, Knowles DM. Paraffin-resistant antigens detectable by antibodies L26 and polyclonal CD3 predict the B- or T-cell lineage of 95% of diffuse aggressive non-Hodgkin's lymphomas. Am J Clin Pathol 1994;102:284-91.
- Tzankov A, Bourgau C, Kaiser A, Zimpfer A, Maurer R, Pileri SA, et al. Rare expression of T-cell markers in classical Hodgkin's lymphoma. Mod Path 2005;18:1542-9.
Peripheral T-cell lymphoma (FFPE) stained with FLEX Anti-CD3, Code IR503/IS503.
- Optimized staining performance of both high and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains
- a guide to diagnostic accuracy.
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Human CD3, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of T cells and related neoplasms (1, 2). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Synthetic peptide comprising amino acids 156-168 from the cytoplasmic part of the human CD3ε-chain coupled to thyroglobulin (1).
In Western blotting, the antibody detects bands of the expected molecular weights for CD3 antigens (2). The antibody recognizes CD3ε in both a T-cell line (Jurkat) and a natural killer cell line (NK11), but does not react with lysates prepared from several B cell lines (Raji, Ramos and JY), a myeloid cell line (U937) or a colon carcinoma cell line (Colo-205) (11).
In immunoprecipitation from Nonidet P40 lysates of surface-iodated T lymphoblasts, the antibody precipitates the γ (26 kDa), δ (21 kDa) and ε (19 kDa) chain of the CD3 molecule, similar to the precipitation pattern seen using the well-characterized monoclonal mouse anti-human CD3, clone UCHT1 (1).
In ELISA, the antibody labels the CD3 peptide used as immunogen.
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
- Mason DY, Cordell J, Brown M, Pallesen G, Ralfkiaer E, Rothbard J, et al. Detection of cells in paraffin wax embedded tissue using antibodies against a peptide sequence from the CD3 antigen. J Clin Pathol 1989; 42:1194-1200.
- Pilozzi E, Pulford K, Jones M, Müller-Hermelink H-K, Falini B, Ralfkiaer E, et al. Co-expression of CD79a (JCB117) and CD3 by lymphoblastic lymphoma. J Pathol 1988; 186:140-3.
- Lanier LL, Chang C, Spitz H, Pillips JH. Expression of cytoplasmic CD3ε proteins in activated human adult natural killer (NK) cells and CD3γ,δ,ε complexes in fetal NK cells. Implications for the relationship of NK and T lymphocytes. J Immunol 1992; 149:1876-80.
CD3 complex, T3