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Monoclonal Mouse Anti-
Cytokeratin 17
Clone E3
Reacts with the 46 kDa human protein corresponding to cytokeratin 17.
The antibody labels the basal layer of complex epithelia, i.e. the basal layer of pseudostratified epithelium in the larynx, trachea and bronchi as well as squamous cell carcinomas of the lung, cervix and oral cavity.
Squamous cell carcinoma (FFPE) stained with FLEX Anti-Cytokeratin 17, Code IR620/IS620.
Lung squamous cell carcinoma. The neoplastic cells show a moderate to strong membranous and cytoplasmic staining reaction.
Clinical application for IR620/IS620. For staining of cytokeratin (CK 17) in myoepithelial cells of human complex epithelia found in various carcinomas.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Cytokeratin 17, Clone E3, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of squamous cell carcinomas (1, 2) and for the identification of pancreatic adenocarcinomas (3) and breast carcinomas (4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Cytokeratin 17, Clone E3, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of squamous cell carcinomas (1, 2) and for the identification of pancreatic adenocarcinomas (3) and breast carcinomas (4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Cytoskeletal fraction of rat colon epithelium (6).
Specificity
In one- and two-dimensional SDS-PAGE and immunoblotting of Triton X-100-extracted cytoskeleton preparations of several cell cultures and tissues, the antibody labeled a band corresponding to human cytokeratin 17 (6).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: E3 (6). Isotype: IgG2b, kappa.
Clone: E3 (6). Isotype: IgG2b, kappa.
References
- Fisseler-Eckhoff A, Erfkamp S, Müller K-M. Cytokeratin expression in preneoplastic lesions and early squamous cell carcinoma of the bronchi. Path Res Pract 1996: 92:552-559.
- Smedts F, Ramaekers F, Link M, Lauerova L, Troyanovsky S, Schijf C, Vooijs G P. Detection of keratin subtypes in routinely processed cervical tissue: implications for tumour classification and the study of cervix cancer aetiology. Virchows Archiv 1994:425:145-155.
- Chu P G, Schwarz R E, Lau S K, Yen Y, Weiss L M. Immunohistochemical staining in the diagnosis of pancreatobiliary and Ampulla of Vater adenocarcinoma. Am J Surg Pathol 2005:29:359-367.
- van de Rijn M, Perou C M, Tibshirani R, Haas P, Kallioniemi O, Kononen J, Torhost J, et al. Expression of cytokeratins 17 and 5 indentifies a group of breast carcinomas with poor clinical outcome. Am J Pathol 2002:161:1991-1996.
- Troyanovsky SM, Guelstein VI, Tchipysheva TA, Krutovskikh VA, Bannikov GA. Patterns of expression of keratin 17 in human epithelia: dependency on cell position. J Cell Sci 1989;93:419-26.
| Product | Size |  | IS620 Clone E3
| 30 tests, 6 mL Product No. IS62030 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Skin | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |