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Monoclonal Mouse Anti-Human
Ki-67 Antigen
Clone MIB-1
With more than 4000 literature citations, the MIB-1 antibody has now been established as the reference monoclonal mouse antibody for the demonstration of the Ki-67 antigen in formalin-fixed, paraffin-embedded specimens.
The Ki-67 antigen is a large nuclear protein (345, 395 kDa) preferentially expressed during all active phases of the cell cycle (G1, S, G2 and M-phases), but absent in resting cells (G0-phase). In diagnostic histopathology and cell biology, antibodies against the Ki-67 antigen have proven valuable by allowing direct monitoring of the growth fraction of normal and neoplastic cells.
- Gerdes J, Becker MH, Key G, Cattoretti G. Immunohistological detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues. J Pathol 1992;168:85-6.
- Cattoretti G, Becker MH, Key G, Duchrow M, Schlüter C, Galle J, et al. Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol 1992;168:357-63.
- Scholzen T, Gerdes J. The Ki-67 protein: from the known and the unknown [review]. J Cell Physiol 2000;182:311-22.
Colon adenocarcinoma (FFPE) stained with FLEX Anti-Ki-67 Antigen, Code IR626/IS626.
Colon adenocarcinoma. The neoplastic cells show a distinct nuclear staining reaction, while only a few normal basal epithelial cells are stained.
Clinical application for IR626/IS626.For identification of proliferating cells in normal and neoplastic tissues such as Burkitt lymphoma/leukemia, colon adenocarcinoma, soft-tissue sarcoma, prostatic adenocarcinoma and breast carcinoma.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
FLEX Monoclonal Mouse Anti-Human Ki-67 Antigen, Clone MIB-1, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of the Ki-67 antigen in normal and neoplastic cells, for example in soft-tissue sarcoma (1), prostatic adenocarcinoma (2), and breast carcinoma (3). Differential identification is aided by the results from a panel of antibodies. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
In immunohistochemistry, the MIB-1 and the Ki-67 antibodies provide identical staining patterns on serial tonsillar frozen sections (8). The MIB-1 antibody recognizes native Ki-67 antigen and recombinant fragments of the Ki-67 molecule (8).
Clone: MIB-1 (8). Isotype: IgG1, kappa.
- Huuhtanen RL, Blomqvist CP, Wiklund TA, Böhling TO, Virolainen MJ, Tukiainen EJ, et al. Comparison of the Ki-67 score and S-phase fraction as prognostic variables in soft-tissue sarcoma. Br J Cancer 1999; 79:945-51.
- Borre M, Bentzen SM, Nerstrøm B, Overgaard J. Tumor cell proliferation and survival in patients with prostate cancer followed expectantly. J Urol 1998; 159:1609-14.
- Seshadri R, Leong AS-Y, McCaul K, Firgaira FA, Setlur V, Horsfall DJ. Relationship between p53 gene abnormalities and other tumor characteristics in breast-cancer prognosis [published erratum appears in Int J Cancer 1996;69:354]. Int J Cancer 1996; 69:135-41.
- Key G, Becker MHG, Baron B, Duchrow M, Schlüter C, Flad H-D, et al. New Ki-67-equivalent murine monoclonal antibodies (MIB 1-3) generated against bacterially expressed parts of the Ki-67 cDNA containing three 62 base pair repetitive elements encoding for the Ki-67 epitope. Lab Invest 1993; 68:629-36.
| Product | Size |  | IS626 Clone MIB-1
| 30 tests, 6 mL Product No. IS62630 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, Low pH (Code K8005) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800521 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Tonsil | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use Low pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |