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Monoclonal Mouse Anti-
Synaptophysin
Clone SY38
Reacts with neuroendocrine cells of the human adrenal medulla, carotid body, skin, pituitary, thyroid, lung, pancreas and gastrointestinal mucosa.
The antibody is useful for the identification of normal neuroendocrine cells and neuroendocrine neoplasms in frozen and short-time formalin-fixed, paraffin-embedded tissue sections.
Small cell lung carcinoma (FFPE) stained with FLEX Anti-Synaptophysin, Code IR776/IS776.
Small cell lung carcinoma. The majority of the neoplastic cells show a strong distinct cytoplasmic and focally dot-like staining reaction.
Clinical application for IR776/IS776 For identification of a wide spectrum of neuroendocrine neoplasms and tumors with neuroendocrine differentiation.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Synaptophysin, Clone SY38, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels presynaptic vesicles of various kinds of neurons, and hence is well suited for the demonstration of synapses (1). Additionally, this antibody is useful for the identification of a wide spectrum of neuroendocrine neoplasms of the neural type, including neuroblastomas, ganglio-neuroblastomas, ganglioneuromas, pheochromocytomas, and paragangliomas. Moreover, this antibody detects synaptophysin in neuroendocrine neoplasms of epithelial type, e.g. pancreatic islet-cell neoplasms, medullary thyroid carcinomas, pituitary and parathyroid adenomas, and bronchopulmonary and gastrointestinal tract carcinoids (2-4). In addition to bronchopulmonary carcinoids, this antibody also identifies neuroendocrine differentiation in lung cancer as demonstrated by its reactivity with cell lines, e.g. from small cell lung cancer, extrapulmonary small cell carcinomas, and non-small cell lung cancers (5). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Synaptophysin, Clone SY38, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels presynaptic vesicles of various kinds of neurons, and hence is well suited for the demonstration of synapses (1). Additionally, this antibody is useful for the identification of a wide spectrum of neuroendocrine neoplasms of the neural type, including neuroblastomas, ganglio-neuroblastomas, ganglioneuromas, pheochromocytomas, and paragangliomas. Moreover, this antibody detects synaptophysin in neuroendocrine neoplasms of epithelial type, e.g. pancreatic islet-cell neoplasms, medullary thyroid carcinomas, pituitary and parathyroid adenomas, and bronchopulmonary and gastrointestinal tract carcinoids (2-4). In addition to bronchopulmonary carcinoids, this antibody also identifies neuroendocrine differentiation in lung cancer as demonstrated by its reactivity with cell lines, e.g. from small cell lung cancer, extrapulmonary small cell carcinomas, and non-small cell lung cancers (5). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Crude fractions of coated vesicles from bovine brain (1).
Specificity
In Western blotting of a fraction of synaptic vesicles of bovine brain, the antibody labels a band of Mr 38 000. Similar results were obtained with corresponding fractions from human, mouse and rat brain (1).
Electron microscopy shows that the antibody almost exclusively labels synaptic termini in brain and spinal cord, and specifically decorates the cytoplasmic surfaces of the presynaptic 40-90 nm vesicles (1).
The epitope recognized by the antibody is located to a pentapeptide repeat structure in the carboxy-terminal cytoplasmic tail of synaptophysin (7).
Electron microscopy shows that the antibody almost exclusively labels synaptic termini in brain and spinal cord, and specifically decorates the cytoplasmic surfaces of the presynaptic 40-90 nm vesicles (1).
The epitope recognized by the antibody is located to a pentapeptide repeat structure in the carboxy-terminal cytoplasmic tail of synaptophysin (7).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: SY38 (1). Isotype: IgG1, kappa.
Clone: SY38 (1). Isotype: IgG1, kappa.
References
- Wiedenmann B, Franke WW. Identification and localization of synap¬tophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell 1985; 41:1017-28.
- Wiedenmann B, Franke WW, Kuhn C, Moll R, Gould VE. Synaptophysin: A marker protein for neuroendocrine cells and neoplasms. Proc Natl Acad Sci (USA) 1986; 83:3500-4.
- Gould VE, Wiedenmann B, Lee I, Schwechheimer K, Dockhorn-Dworniczak B, Radosevich JA, et al. Synaptophysin expression in neuroendocrine neoplasms as determined by immunocytochemistry. Am J Pathol 1987; 126:243-57.
- Stefaneanu L, Ryan N, Kovacs K. Immunocytochemical localization of synap¬tophysin in human hypophyses and pituitary adenomas. Arch Pathol Lab Med 1988; 112:801-4.
- Jensen SM, Gazdar AF, Cuttitta F, Russell EK, Linnoila RI. A comparison of synaptophysin, chromogranin, and L-dopa decarboxylase as markers for neuroendocrine differentiation in lung cancer cell lines. Cancer Res 1990; 50:6068-74.Navone F, Jahn R, Di Gioia G, Stukenbrok H, Greengard P, De Camilli P. Protein p38: An integral membrane protein specific for small vesicles of neurons and neuroendocrine cells. J Cell Biol 1986; 103:2511-27.
- Knaus P, Betz H. Mapping of a dominant immunogenic region of synaptophysin, a major membrane protein of synaptic vesicles. FEBS Lett 1990; 261:358-60.
| Product | Size |  | IS776 Clone SY38
| 30 tests, 6 mL Product No. IS77630 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Colon | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
Synonym(s) for Antigen
protein p38