H. pylori (formerly called Campylobacter pylori) is a spiral-shaped microaerophilic, Gram-negative rod with unipolar sheated flagella.
It inhabits the mucus lining of the gastric epithelium. H. pylori is one of the most common pathogenic infections, which leads to serious gastroduodenal diseases in a subset of individuals. There is evidence supporting an association between H. pylori and chronic atrophic gastritis as well as gastric cancer. This antibody is useful for the identification of infections with H. pylori in gastritis and gastric cancer.
Gastric mucosa (FFPE) stained with FLEX Anti-Helicobacter Pylori, Code IR523/IS523.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains
- a guide to diagnostic accuracy
For in vitro diagnostic use.
FLEX Polyclonal Rabbit Anti-Helicobacter Pylori, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of infections with H. pylori in gastritis and gastric cancer (1-4). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
The antigen for immunization was prepared from heat-treated cells of the H. pylori strain, CH-20426 (1, 2).
The antibody labels heat-stable, somatic antigens of the whole H. pylori organism. Traces of contaminating antibodies have been found by solid-phase absorption with human plasma proteins.
In crossed immunoeletrophoresis, using 12.5 µL concentrated antibody per cm2 gel area against 2 µL of human plasma no precipitate appears. Staining: Coomassie Brilliant Blue.
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.1 mol/L NaCl, 0.015 mol/L NaN3.
- Anderson LP, Holck S, Povlsen CO. Campylobacter pylori detected by indirect immunohistochemical technique. Ampis 1988;96:559-64.
- Anderson LP, Holck S. Possible evidence of invasiveness of Helicobacter (Campylobacter) pylori. Eur J Clin Microbiol Infect Dis 1990;9:135-8.
- Jonkers D, Gisbertz I, de Bruine A, Bot F, Arends JW, Stobberingh E, et al. Helicobacter pylori and non- Helicobacter pylori bacterial flora in gastric mucosal and tumor specimens of patients with primary gastric lymphoma. Eur J Clin Invest 1997;27:885-92.
- Jonkers D, Stobberingh E, de Bruine A, Arends JW, Stockbrügger R. Evaluation of Immunohistochemistry for the detection of Helicobacter pylori in gastric mucosal biopsies. J Infect 1997;35:149-54.