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Monoclonal Mouse Anti-Human
Cytokeratin 20
Clone Ks20.8
Reacts with the 46 kDa protein corresponding to cytokeratin 20.
The antibody labels the majority of adenocarcinomas of the colon, mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas, and frequently adenocarcinomas of the stomach, bile system and pancreas. Most squamous cell carcinomas and most adenocarcinomas from other sites (breast, endometrium, lung, prostate), non-mucinous tumors of the ovary, and small-cell lung carcinomas are essentially or completely negative.
Isotype IgG2a, kappa
- Formalin
- Enzyme/HIER
References
- Moll R, Löwe A, Laufer J, Franke WW. Cytokeratin 20 in human carcinomas. Am J Pathol 1992;140:427-47.
- Wauters CCAP, Smedts F, Gerrits LGM, Bosman FT, Ramaekers FCS. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary. Hum Pathol 1995;26:852-5.
- Harnden P, Mahmood N, Southgate J. Expression of cytokeratin 20 redefines urothelial papillomas of the bladder. Lancet 1999;353:974-7.
- Chu P, Wu W, Weiss LM. Cytokeratin 7 and cytokeratin 20 expression in epithelial neoplasms: a survey of 435 cases. Mod Pathol 2000;13:962-72.
Colon adenocarcinoma (FFPE) stained with FLEX Anti-Cytokeratin 20, Code IR777/IS777.
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Colon adenocarcinoma. The majority of the neoplastic cells show a strong cytoplasmic staining reaction.
Clinical application for IR777/IS777. Primarily for identification of gastrointestinal tumors, mucinous ovarian tumors and Merkel cell carcinoma. Anti-Cytokeratin 20 stains the normal gastrointestinal epithelium and urothelium.
Merkel cell carcinoma. The majority of the neoplastic cells show a perinuclear cytoplasmic and dot-like staining reaction.
Clinical application for IR777/IS777. Primarily for identification of gastrointestinal tumors, mucinous ovarian tumors and Merkel cell carcinoma. Anti-Cytokeratin 20 stains the normal gastrointestinal epithelium and urothelium.
Colon. The luminal cells show a moderate to strong staining reaction, whereas the epithelial cells of the basal crypts show a weak to moderate staining reaction. Endocrine cells of the basal crypt show a moderate to strong staining reaction.
Recommended control for IR777/IS777. Appendix/Colon.
Colon stained with Anti-Cytokeratin 20.
Recommended control for IR777/IS777. Appendix/Colon.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Human Cytokeratin 20, Clone Ks 20.8, is intended for use in immunohistochemistry. Cytokeratin 20 (CK 20) expression is almost entirely confined to normal and abnormal gastric and intestinal epithelium, urothelium and Merkel cells (1, 2), and the antibody is of particular value in the classification of carcinomas originating from these cell types (1, 3-5). Differential identification is aided by the results from a panel of antibodies. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist
Monoclonal Mouse Anti-Human Cytokeratin 20, Clone Ks 20.8, is intended for use in immunohistochemistry. Cytokeratin 20 (CK 20) expression is almost entirely confined to normal and abnormal gastric and intestinal epithelium, urothelium and Merkel cells (1, 2), and the antibody is of particular value in the classification of carcinomas originating from these cell types (1, 3-5). Differential identification is aided by the results from a panel of antibodies. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist
Immunogen
CK20 isolated by SDS-PAGE from cytoskeletal material obtained from human duodenal mucosa (1).
Specificity
Anti-Human CK 20, Clone Ks 20.8, labels a 46 kDa polypeptide corresponding to CK 20 in Western immunoblotting of SDS-PAGE-separated cytoskeletal proteins from human duodenal mucosa (1).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as purified mouse IgG from ascitic fluid. In 0.05 mol/L Tris/HCL, 15 mmol/L NaN3, 1 % bovine serum albumin, pH 7.2.
Clone: Ks 20.8 (1). Isotype: IgG2a, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
Clone: Ks 20.8 (1). Isotype: IgG2a, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
References
- Moll R, Löwe A, Laufer J, Franke WW. Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol 1992;140:427-47.
- Moll R. Cytokeratins as markers of differentiation in the diagnosis of epithelial tumors. In: Herrmann, Harris, editors. Subcellular biochemistry. Volume 31, New York: Plenum Press; 1998. p. 205-62.
- Savera AT, Torres FX, Linden MD, Bacchi CE, Gown AM, Zarbo RJ. Primary versus metastatic pulmonary adenocarcinoma. An immunohistochemical study using villin and cytokeratins 7 and 20. Appl Immunohistochem 1996;4:86-94.
- Harnden P, Mahmood N, Southgate J. Expression of cytokeratin 20 redefines urothelial papillomas of the bladder. Lancet 1999; 353:974-77.
- Wauters CCAP, Smedts F, Gerrits LGM, Bosman FT, Ramaekers FCS. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary. Hum Pathol 1995;26:852-55.
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| Product | Size |  | M7019 Purified
| 0.2 mL Product No. M701929-2 |
|---|---|---|
| 1 mL Product No. M701901-2 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Cytokeratin 20, Clone Ks20.8, Ready-to-Use, (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels normal and abnormal gastric and intestinal epithelium, urothelium and Merkel cells (1, 2), and this antibody is of particular value in the classification of carcinomas originating from these cell types (1, 3-5). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Cytokeratin 20, Clone Ks20.8, Ready-to-Use, (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody labels normal and abnormal gastric and intestinal epithelium, urothelium and Merkel cells (1, 2), and this antibody is of particular value in the classification of carcinomas originating from these cell types (1, 3-5). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
CK20 isolated by SDS-PAGE from cytoskeletal material obtained from human duodenal mucosa (1).
Specificity
In Western immunoblotting of SDS-PAGE-separated cytoskeletal proteins from human duodenal mucosa, the antibody labels a 46 kDa polypeptide corresponding to CK20 (1).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: Ks20.8 (1). Isotype: IgG2a, kappa.
Clone: Ks20.8 (1). Isotype: IgG2a, kappa.
References
- Moll R, Löwe A, Laufer J, Franke WW. Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol 1992; 140:427-47.
- Moll R. Cytokeratins as markers of differentiation in the diagnosis of epithelial tumors. In: Herrmann, Harris, editors. Subcellular biochemistry. Volume 31, New York: Plenum Press; 1998. p. 205-62.
- Savera AT, Torres FX, Linden MD, Bacchi CE, Gown AM, Zarbo RJ. Primary versus metastatic pulmonary adenocarcinoma. An immunohistochemical study using villin and cytokeratins 7 and 20. Appl Immunohistochem 1996; 4:86-94.
- Harnden P, Mahmood N, Southgate J. Expression of cytokeratin 20 redefines urothelial papillomas of the bladder. Lancet 1999; 353:974-77.
- Wauters CCAP, Smedts F, Gerrits LGM, Bosman FT, Ramaekers FCS. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary. Hum Pathol 1995; 26:852-55.
Check Your Price & Order Online
| Product | Size | | IR777 RTU, FLEX
| 60 tests, 12 mL Product No. IR77761-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Colon | 120 tests, 24 mL Product No. IR75066-2 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021-2 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Human Cytokeratin 20, Clone Ks20.8, Ready-to-Use, (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels normal and abnormal gastric and intestinal epithelium, urothelium and Merkel cells (1, 2), and this antibody is of particular value in the classification of carcinomas originating from these cell types (1, 3-5). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Human Cytokeratin 20, Clone Ks20.8, Ready-to-Use, (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody labels normal and abnormal gastric and intestinal epithelium, urothelium and Merkel cells (1, 2), and this antibody is of particular value in the classification of carcinomas originating from these cell types (1, 3-5). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
CK20 isolated by SDS-PAGE from cytoskeletal material obtained from human duodenal mucosa (1).
Specificity
In Western immunoblotting of SDS-PAGE-separated cytoskeletal proteins from human duodenal mucosa, the antibody labels a 46 kDa polypeptide corresponding to CK20 (1).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: Ks20.8 (1). Isotype: IgG2a, kappa.
Clone: Ks20.8 (1). Isotype: IgG2a, kappa.
References
- Moll R, Löwe A, Laufer J, Franke WW. Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol 1992; 140:427-47.
- Moll R. Cytokeratins as markers of differentiation in the diagnosis of epithelial tumors. In: Herrmann, Harris, editors. Subcellular biochemistry. Volume 31, New York: Plenum Press; 1998. p. 205-62.
- Savera AT, Torres FX, Linden MD, Bacchi CE, Gown AM, Zarbo RJ. Primary versus metastatic pulmonary adenocarcinoma. An immunohistochemical study using villin and cytokeratins 7 and 20. Appl Immunohistochem 1996; 4:86-94.
- Harnden P, Mahmood N, Southgate J. Expression of cytokeratin 20 redefines urothelial papillomas of the bladder. Lancet 1999; 353:974-77.
- Wauters CCAP, Smedts F, Gerrits LGM, Bosman FT, Ramaekers FCS. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary. Hum Pathol 1995; 26:852-55.
Check Your Price & Order Online
| Product | Size | | IS777 RTU, FLEX
| 30 tests, 6 mL Product No. IS77730-2 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421-2 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Colon | 60 tests, 12 mL Product No. IS75061-2 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021-2 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821-2 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021-2 |
Synonym(s) for Antigen
protein IT, CK20, keratin