Monoclonal Mouse Anti-Human

Wilms' Tumor 1 (WT1) Protein

Clone 6F-H2

WT1 is a gene involved in the induction of Wilms' tumor, a pediatric renal malignancy.

Wilms' tumor 1 protein regulates transcription of other genes and can function both as a transcriptional activator and repressor. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilms' tumors. WT1 expression has been demonstrated in the epithelial and blastemal components of Wilms' tumors and in the majority of malignant mesotheliomas as well as the majority of acute leukemias.

Serous ovarian adenocarcinoma (FFPE) stained with FLEX Anti-Wilms' Tumor 1 (WT1) Protein, Code IR055/IS055.

+

Ovarian serous adenocarcinoma. The majority of the neoplastic cells show a distinct nuclear staining reaction. A weak cytoplasmic staining reaction can also be seen in some cells.

Clinical application for IR055/IS055 For identification of Wilms' tumor, malignant mesothelioma and serous ovarian adenocarcinoma.

Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.

  • Optimized staining performance of both high- and low-expression structures
  • Crisp and clear staining with no background
  • Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy

Intended Use
For in vitro diagnostic use.

FLEX Monoclonal Mouse Anti-Human Wilms' Tumor (WT1), Clone 6F-H2, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of Wilms tumor, acute leukemias, malignant mesothelioma, serous ovarian adenocarcinoma, granulosa cell tumor, and other tumors. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Truncated human WT1 protein corresponding to N-terminal amino acids 1-181 (1,2)
Specificity
Alternate splicing of the WT1 transcript gene results in four distinct mRNA species which are present in different amounts. The predicted molecular weight of the WT1 protein ranges from 46 to 49 kDa depending on the mRNA species however, the WT1 proteins derived from cells migrate at 50 to 55 kDa in SDS-PAGE (4). This antibody recognizes an epitope found in the amino terminal 84 encoded amino acids of WT1. It reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2, frequently found in subsets of sporadic Wilms' tumors. In immunoprecipitation assays, the antibody has been shown to recognize full-length and in vitro translated WT1. It also detects full-length denatured WT1 (55 kDa) in Western blots of WT1 baculovirus-infected cell lysates (1).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.

Clone: 6F-H2 (1,2). Isotype: IgG1, kappa.
References
  1. Rauscher JF, et al. Submitted for publication.
  2. Menssen HD, et al. Presence of Wilms' tumor (wt1) transcripts and the WT1 nuclear protein in the majority of human acute leukemias. Leukemia 1995; 9:1060
  1. Haber DA and Buckler AJ. WT1: A novel tumor suppressor gene inactivated in Wilms' tumor. New Biol 1992; 4(2):97
ProductSize
This product is CE-marked according to the EU IVD Directive 98/79/ECIR055 Clone 6F-H2
Specification Sheet
Safety Data Sheet:
Non-hazard classification statement
60 tests, 12 mL
Product No. IR05561-2

RECOMMENDED COMPANION PRODUCTS

StepProductSize
Pre-treatmentEnVision™ FLEX Target Retrieval Solution, High pH (Code K8004)
3-in-1 procedure using PT Link
3 x 30 mL
Product No. K800421-2
Antibody Dilution
Ready-to-use / Prediluted
 
Negative ControlFLEX Negative Control, Mouse (Code IR750)
Positive tissue: Epithelial cells and the majority of the smooth muscle cells
120 tests, 24 mL
Product No. IR75066-2
Visualization SystemEnVision™ FLEX (Code K8000)
Use High pH Target Retrieval Solution
400-600 tests
Product No. K800021-2
CounterstainHematoxylin (Link) (Code K8008)
Non-aqueous, permanent mounting medium is required
3 x 45 mL, 400-600 tests
Product No. K800821-2
Glass SlidesSilanized Slides (Code S3003)
Recommended for greater adherence
Alternative is FLEX IHC Microscope Slides (Code K8020)
100 slides
Product No. S300330-2

Country selection

Your Dako Account indicates that you are based in , you will be transfered to the web site for

Basket emptied

Your basket was emptied because of the country change.

Country selection

United States of America appears to be your location. To view country specific information on the Dako web site, please accept or change country below.



Get in Touch with Dako in Finland

  • Pathology Products
    Algol Diagnostics Oy

    orders@algoldiagnostics.fi
    +358 9 50 991
    +358 9 595 006

    P.O. Box 13
    FI-02611 Espoo

  • Flow Cytometry Products and Specific Proteins
    Electra-Box Diagnostica Oy

    +358 (0)9 7244330
    +358 (0)9 7244331

    Lyhtytie 8
    00750 Helsinki

Detailed contact information

Submit an Inquiry

How would you like us to follow up?


Cancel

Your session expired

You have been logged out. Log in again to retrieve your Cart.