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Monoclonal Mouse Anti-
Vimentin
Clone V9
Vimentin is a 57 kDa intermediate filament protein which forms part of the cytoskeleton of vertebrate cells and is characteristically found in cells of mesenchymal origin.
The coexpression of intermediate filaments, particularly vimentin and cytokeratin, has been demonstrated in a variety of normal cells/tissues and in neoplastic lesions, necessitating the use of antibodies against other types of intermediate filaments. The antibody is useful for the identification of cells of mesenchymal origin in normal and neoplastic tissues.
Isotype IgG1, kappa
- Frozen
- Formalin
- HIER
B-cell chronic lymphocytic leukemia (FFPE) stained with FLEX Anti-Vimentin, Code IR630/IS630.
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B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma. The majority of the neoplastic cells show a distinct cytoplasmic staining reaction
Clinical application for IR630/IS630. For identification of cells of mesenchymal origin in normal and neoplastic tissues such as B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma.
Tonsil. Lymphocytes and endotehelial cells show a strong cytoplasmic staining reaction. Stromal cells and endothelial cells should be demonstrated.
Recommended control for IR630/IS630. Tonsil/Liver/Appendix/Colon.
Liver. The macrophages and lymphocytes show a weak to moderate staining reaction, while the hepatocytes are negative.
Recommended control for IR630/IS630. Tonsil/Liver/Appendix/Colon.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Concentrated Antibody
for Manual Use - Ready-to-use Antibody
for Autostainer Link Instrument - Ready-to-use Antibody
for Autostainer Plus Instrument
- Excellent specificity
- High lot to lot consistency
- Optimized for immunohistochemistry (IHC) with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Monoclonal Mouse Anti-Vimentin, Clone V9, is intended for use in immunohistochemistry. The antibody labels primarily cells of mesenchymal origin in normal and neoplastic tissues, and is of value in tumor diagnosis. Differential identification is aided by the results from a panel of antibodies - especially antibodies against other types of intermediate filaments (1). Interpretation must be made within the context of the patient´s clinical history and other diagnostic tests by a qualified pathologist.
Monoclonal Mouse Anti-Vimentin, Clone V9, is intended for use in immunohistochemistry. The antibody labels primarily cells of mesenchymal origin in normal and neoplastic tissues, and is of value in tumor diagnosis. Differential identification is aided by the results from a panel of antibodies - especially antibodies against other types of intermediate filaments (1). Interpretation must be made within the context of the patient´s clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Purified vimentin from porcine eye lens (3).
Specificity
In Western blotting of purified porcine vimentin, the antibody labels a single band of 57 kDa corresponding to vimentin. When applying whole cell extracts of cell lines expressing vimentin plus glial fibrillary acidic protein (GFAP), and vimentin plus desmin, respectively, the antibody labels specifically the 57 kDa vimentin band. As directly shown by these experiments, the antibody does not react with the two IF proteins most closely related to vimentin, i.e. desmin and GFAP (3).
In immunocytochemistry the antibody labels the vimentin-positive human cell lines IMR90, RD, glioma and HeLa (3).
As demonstrated by immunohistochemistry, the antibody cross-reacts with the vimentin-equivalent protein in man, cow, dog, hamster, horse, rhesus and African green monkey, rabbit, rat and rat kangaroo. No cross-reaction with mouse vimentin could be demonstrated, and results on chicken specimens are contradictory (3, 4).
In immunocytochemistry the antibody labels the vimentin-positive human cell lines IMR90, RD, glioma and HeLa (3).
As demonstrated by immunohistochemistry, the antibody cross-reacts with the vimentin-equivalent protein in man, cow, dog, hamster, horse, rhesus and African green monkey, rabbit, rat and rat kangaroo. No cross-reaction with mouse vimentin could be demonstrated, and results on chicken specimens are contradictory (3, 4).
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3.
Clone: V9 (3). Isotype: IgG1, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
Clone: V9 (3). Isotype: IgG1, kappa.
Mouse IgG concentration: see label on vial.
The protein concentration between lots may vary without influencing the optimal dilution. The titer of each individual lot is compared and adjusted to a reference lot to ensure a consistent immunohistochemical staining performance from lot-to-lot.
References
- Azumi N, Battifora H. The distribution of vimentin and keratin in epithelial and nonepithelial neoplasms. Am J Clin Pathol 1987;88:286-96.
- Osborn M, Debus E, Weber K. Monoclonal antibodies specific for vimentin. Eur J Cell Biol 1984;34:137-43.
- Bohn W, Wiegers W, Beuttenmüller M, Traub P. Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp Cell Res 1992;201:1-7.
| Product | Size |  | M0725 Culture supernatant
| 0.2 mL Product No. M072529 |
|---|---|---|
| 1 mL Product No. M072501 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Vimentin, Clone V9, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of cells of mesenchymal origin in normal and neoplastic tissues, and is of value in tumor diagnosis. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Vimentin, Clone V9, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. This antibody is useful for the identification of cells of mesenchymal origin in normal and neoplastic tissues, and is of value in tumor diagnosis. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Purified vimentin from porcine eye lens (3).
Specificity
In Western blotting of purified porcine vimentin, the antibody labels a single band of 57 kDa corresponding to vimentin. When applying whole cell extracts of cell lines expressing vimentin plus glial fibrillary acidic protein (GFAP), and vimentin plus desmin, respectively, the antibody labels specifically the 57 kDa vimentin band. As directly shown by these experiments, the antibody does not react with the two IF proteins most closely related to vimentin, i.e. desmin and GFAP (3).
In immunocytochemistry the antibody labels the vimentin-positive human cell lines IMR90, RD, glioma and HeLa (3).
In immunocytochemistry the antibody labels the vimentin-positive human cell lines IMR90, RD, glioma and HeLa (3).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
Clone: V9 (3). Isotype: Ig1, kappa.
Clone: V9 (3). Isotype: Ig1, kappa.
References
- Osborn M, Debus E, Weber K. Monoclonal antibodies specific for vimentin. Eur J Cell Biol 1984;34:137-43.
| Product | Size | | IR630 RTU, FLEX
| 60 tests, 12 mL Product No. IR63061 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Tonsil and liver | 120 tests, 24 mL Product No. IR75066 |
| Visualization System | EnVision™ FLEX (Code K8000) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800021 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti-Vimentin, Clone V9, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of cells of mesenchymal origin in normal and neoplastic tissues, and is of value in tumor diagnosis. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti-Vimentin, Clone V9, Ready-to-Use (Dako Autostainer/Autostainer Plus), is intended for use in immunohistochemistry together with Dako Autostainer/Autostainer Plus instruments. This antibody is useful for the identification of cells of mesenchymal origin in normal and neoplastic tissues, and is of value in tumor diagnosis. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Purified vimentin from porcine eye lens (3).
Specificity
In Western blotting of purified porcine vimentin, the antibody labels a single band of 57 kDa corresponding to vimentin. When applying whole cell extracts of cell lines expressing vimentin plus glial fibrillary acidic protein (GFAP), and vimentin plus desmin, respectively, the antibody labels specifically the 57 kDa vimentin band. As directly shown by these experiments, the antibody does not react with the two IF proteins most closely related to vimentin, i.e. desmin and GFAP (3).
In immunocytochemistry the antibody labels the vimentin-positive human cell lines IMR90, RD, glioma and HeLa (3).
In immunocytochemistry the antibody labels the vimentin-positive human cell lines IMR90, RD, glioma and HeLa (3).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
Clone: V9 (3). Isotype: IgG1, kappa.
Clone: V9 (3). Isotype: IgG1, kappa.
References
- Osborn M, Debus E, Weber K. Monoclonal antibodies specific for vimentin. Eur J Cell Biol 1984;34:137-43.
| Product | Size | | IS630 RTU, FLEX
| 30 tests, 6 mL Product No. IS63030 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IS750) Positive tissue: Tonsil and liver | 60 tests, 12 mL Product No. IS75061 |
| Visualization System | EnVision™ FLEX (Code K8010) Use High pH Target Retrieval Solution | 400-600 tests Product No. K801021 |
| Counterstain | Hematoxylin (Dako Autostainer/Autostainer Plus) (Code K8018) Non-aqueous, permanent mounting medium is required | 10 x 13 mL, 400-600 tests Product No. K801821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |
Synonym(s) for Antigen
V9