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Polyclonal Rabbit Anti-
Glial Fibrillary Acidic Protein (GFAP)
GFAP isolated from bovine spinal cord has been used for immunization. Z0334 reacts strongly with human GFAP and with GFAP in many animal species tested: cat, cow, dog, mouse, rat and sheep.
In the central nervous system Z0334 labels astrocytes and some groups of ependymal cells. In the peripheral nervous system Schwann's cells, satellite cells and enteric glial cells are labeled. Negative labeling is found in skin, connective tissue, lymphatic tissue, muscle, gastrointestinal tract, including liver and pancreas, kidney, ureter and bladder.
Z0334 is useful particularly for distinguishing cells of astrocytic origin in the central nervous system. The antibody can be used on frozen and formalin-fixed, paraffin-embedded tissue sections.
- Precipitation
- IHC
- Excellent specificity and precipitation ability
- High lot to lot consistency
- Optimized for a variety of applications with validated protocols
- Certified manufacturing facilities guarantee full quality control
Intended Use
For in vitro diagnostic use.
Polyclonal Rabbit Anti-Glial Fibrillary Acidic Protein, is intended for use in immunohistochemistry. Glial fibrillary acidic protein (GFAP) is the principal intermediate filament of mature astrocytes (1), and the antibody is a useful tool e.g. for the identification of astrocytes in the central nervous system (CNS) under normal and pathological conditions (2-4). Differential identification is aided by the results from a panel of antibodies. Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Polyclonal Rabbit Anti-Glial Fibrillary Acidic Protein, is intended for use in immunohistochemistry. Glial fibrillary acidic protein (GFAP) is the principal intermediate filament of mature astrocytes (1), and the antibody is a useful tool e.g. for the identification of astrocytes in the central nervous system (CNS) under normal and pathological conditions (2-4). Differential identification is aided by the results from a panel of antibodies. Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional.
Immunogen
GFAP isolated from cow spinal cord.
Specificity
The antibody has been solid-phase absorbed with human and cow serum proteins.
In crossed immunoelectrophoresis using 50 µL antibody per cm2 gel area, no reaction with 2 µL human plasma and 2 µL cow serum is observed. The antibody shows one distinct precipitate (GFAP) with cow brain extract. Staining: Coomassie Brilliant Blue.
In indirect ELISA, the antibody shows no reaction with human plasma and cow serum.
GFAP shows 90-95% homology between species (5), and as demonstrated by immunohistochemistry, the antibody cross-reacts with GFAP in cat (7), dog (7), mouse (8), rat (8), and sheep (9). Additionally, it reacts strongly with human and cow GFAP.
In crossed immunoelectrophoresis using 50 µL antibody per cm2 gel area, no reaction with 2 µL human plasma and 2 µL cow serum is observed. The antibody shows one distinct precipitate (GFAP) with cow brain extract. Staining: Coomassie Brilliant Blue.
In indirect ELISA, the antibody shows no reaction with human plasma and cow serum.
GFAP shows 90-95% homology between species (5), and as demonstrated by immunohistochemistry, the antibody cross-reacts with GFAP in cat (7), dog (7), mouse (8), rat (8), and sheep (9). Additionally, it reacts strongly with human and cow GFAP.
Reagent Provided
Purified immunoglobulin fraction of rabbit antiserum provided in liquid form. In 0.1 mol/L NaCl, 15 mmol/L NaN3, pH 7.2.
Protein concentration g/L: See label on vial.
The antibody titre variation between different lots is less than 10% as measured by single radial immunodiffusion. This is achieved by adjusting the titre of each individual lot to match the titre of a reference preparation kept at -80 °C.
Protein concentration g/L: See label on vial.
The antibody titre variation between different lots is less than 10% as measured by single radial immunodiffusion. This is achieved by adjusting the titre of each individual lot to match the titre of a reference preparation kept at -80 °C.
References
- Eng LF, Ghirnikar RS, Lee YL. Glial fibrillary acidic protein: GFAP-thirty-one years (1969-2000). Neurochem Res 2000;25:1439-51.
- Reske-Nielsen E, Oster S, Reintoft I. Astrocytes in the prenatal central nervous system. Acta path microbiol immunol scand Sect. A 1987;95:339-46.
- Oh D, Prayson RA. Evaluation of epithelial and keratin markers in glioblastoma multiforme. An immuno-histochemical study. Arch Pathol Lab Med 1999;123:917-20.
- Geddes JF, Thom M, Robinson SFD, Revesz T. Granular cell change in astrocytic tumors. Am J Surg Pathol 1996;20:55-63.
- Rutka JT, Murakami M, Dirks PB, Hubbard SL, Becker LE, Fukuyama K, et al. Role of glial filaments in cells and tumors of glial origin: a review. J Neurosurg 1997;87:420-30.
- Boya J, Calvo JL. Immunohistochemical study of the pineal astrocytes in the postnatal development of the cat and dog pineal gland. J Pineal Res 1993;15:13-20.
- Castellano B, Gonzales B, Jensen MB, Pedersen EB, Finsen BR, Zimmer J. A double staining technique for simultaneous demonstration of astrocytes and microglia in brain sections and astroglial cell cultures. J Histochem Cytochem 1991;39:561-8.
- Hansen SH, Stagaard M, Møllgård K. Neurofilament-like pattern of reactivity in human foetal PNS and spinal cord following immunostaining with polyclonal anti-glial fibrillary acidic protein antibodies. J Neurocytol 1989;18:427-36.
| Product | Size |  | Z0334 Ig fraction
| 0.2 mL Product No. Z033429 |
|---|---|---|
| 1 mL Product No. Z033401 |