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Monoclonal Mouse Anti-Human
Estrogen Receptor α
Clone 1D5
Reacts with estrogen receptor alpha.
The antibody strongly labels the nuclei of cells known to contain a high level of estrogen receptor, e.g. epithelial and myometrial cells of the uterus, and normal, hyperplastic and neoplastic cells of the mammary gland. A number of publications has confirmed the usefulness of the Dako antibody.
For DakoLink users:
Go to the Autostainer Link Installer section and download the Installer for Code IR657, ER alpha 1D5 Protocol Updater. Follow the instructions to complete the update.
References
- Al Saati T, Clamens S, Cohen-Knafo E, Faye JC, Prats H, Coindre JM, et al. Production of monoclonal antibodies to human estrogen-receptor protein (ER) using recombinant ER (RER). Int J Cancer 1993;55:651-4.
- Sannino P, Shousha S. Demonstration of oestrogen receptors in paraffin wax sections of breast carcinoma using the monoclonal antibody 1D5 and microwave oven processing. J Clin Pathol 1994;47:90-2.
- Jotti GS, Johnston SRD, Salter J, Detre S, Dowsett M. Comparison of immunohistochemical assay for oestrogen receptor in paraffin wax embedded breast carcinoma tissue with quantitative enzyme immunoassay. J Clin Pathol 1994;47:900-5.
- Goulding H, Pinder S, Cannon P, Pearson D, Nicholson R, Snead D, et al. An immunohistochemical antibody for the assessment of estrogen receptor status on routine formalin-fixed tissue samples. Hum Pathol 1995;26:291-4.
- Hendricks JB, Stephen CA, Wilkinson EJ, Szekeres G. Estrogen receptor specific (ER1D5) epitope stability in paraffin sections. J Histotechnol 1996;19:23-5.
- Taylor CR. Paraffin section immunocytochemistry for estrogen receptor. The time has come (editorial). Cancer 1996; 77:2419-22.
Breast ductal carcinoma (FFPE) stained with FLEX Anti-Estrogen Receptor α, Code IR657/IS657.
Breast ductal carcinoma. The majority of the neoplastic cells show a distinct nuclear staining reaction.
Clinical application for IR657/IS657. For assessment of estrogen receptor status in breast carcinomas.
Disclaimer: Due to variances among color computer monitors, the colors you see on your screen may not be the exact colors of the stain. The image you see should be considered close renditions to the actual stain.
- Optimized staining performance of both high- and low-expression structures
- Crisp and clear staining with no background
- Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Link 48
Intended Use
For in vitro diagnostic use.
FLEX Monoclonal Mouse Anti- Human Estrogen Receptor α, Clone 1D5, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. The antibody labels estrogen receptor α-positive cells and is useful in the assessment of estrogen receptor status in human breast carcinomas (1-3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
FLEX Monoclonal Mouse Anti- Human Estrogen Receptor α, Clone 1D5, Ready-to-Use (Link), is intended for use in immunohistochemistry together with Autostainer Link instruments. The antibody labels estrogen receptor α-positive cells and is useful in the assessment of estrogen receptor status in human breast carcinomas (1-3). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Immunogen
Soluble recombinant human estrogen receptor (7).
Specificity
The antibody specifically reacts with ERα and shows no reaction with ERβ (8, 9). The epitope is located in the N-terminal domain (A/B region) of ERα. In Western immunoblotting using protein extracts from COS cells transfected with a plasmid vector expressing ER, the human breast cancer cell line MCF-7, and human endometrial tissue, the antibody labels a polypeptide of approximately 67 kDa corresponding to ER. No labeling of COS cells transfected with plasmid containing a deleted mutant with missing sequences encoding for the A/B region of ER was observed (7).
As demonstrated by immunohistochemistry, the antibody reacts with the ERα-equivalent protein in rat (8).
As demonstrated by immunohistochemistry, the antibody reacts with the ERα-equivalent protein in rat (8).
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: 1D5 (7). Isotype: IgG1, kappa.
Clone: 1D5 (7). Isotype: IgG1, kappa.
References
- Mauri FA, Veronese S, Frigo B, Girlando S, Losi L, Gambacorta M, et al. ER1D5 and H222 (ER-ICA) antibodies to human estrogen receptor protein in breast carcinomas. Appl Immunohistochem 1994;2:157-63.
- Goulding H, Pinder S, Cannon P, Pearson D, Nicholson R, Snead D, et al. A new immunohistochemical antibody for the assessment of estrogen receptor status on routine formalin-fixed tissue samples. Hum Pathol 1995;26:291-4.
- Shaw JA, Udokang K, Mosquera J-M, Chauhan H, Jones JL, Walker RA. Oestrogen receptors alpha and beta differ in normal human breast and breast carcinomas. J Pathol 2002;198:450-7.
- Al Saati T, Clamens S, Cohen-Knafo E, Faye JC, Prats H, Coindre JM, et al. Production of monoclonal antibodies to human estrogenreceptor protein (ER) using recombinant ER (RER). Int J Cancer 1993;55: 651-4.
- Nishihara E, Nagayama Y, Inoue S, Hiroi H, Muramatsu M, Yamashita S, et al. Ontogenetic changes in the expression of estrogen receptor α and β in rat pituitary gland detected by immunohistochemistry. Endocrinology 2000;141:615-20.
- Pettersson K, Grandien K, Kuiper GGJM, Gustafsson J-Å. Mouse estrogen receptor β forms estrogen response element-binding heterodimers with estrogen receptor α. Mol Endocrinol 1997;11:1486-96
| Product | Size |  | IR657 Clone 1D5
| 60 tests, 12 mL Product No. IR65760 |
|---|
RECOMMENDED COMPANION PRODUCTS
| Step | Product | Size |
|---|---|---|
| Pre-treatment | EnVision™ FLEX Target Retrieval Solution, High pH (Code K8004) 3-in-1 procedure using PT Link | 3 x 30 mL Product No. K800421 |
| Antibody Dilution | Ready-to-use / Prediluted | |
| Negative Control | FLEX Negative Control, Mouse (Code IR750) Positive tissue: Cervix | 120 tests, 24 mL Product No. IR75066 |
| Visualization System | EnVision™ FLEX+ (Code K8002) Use High pH Target Retrieval Solution | 400-600 tests Product No. K800221 |
| Counterstain | Hematoxylin (Link) (Code K8008) Non-aqueous, permanent mounting medium is required | 3 x 45 mL, 400-600 tests Product No. K800821 |
| Glass Slides | FLEX IHC Microscope Slides (Code K8020) Recommended for greater adherence | 5 x 100 slides Product No. K802021 |